Solution structure of the complexed RCD1-RST
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 75.2 % (671 of 892) | 73.3 % (344 of 469) | 75.5 % (262 of 347) | 85.5 % (65 of 76) |
Backbone | 86.7 % (371 of 428) | 88.7 % (126 of 142) | 83.9 % (183 of 218) | 91.2 % (62 of 68) |
Sidechain | 67.4 % (361 of 536) | 66.7 % (218 of 327) | 69.7 % (140 of 201) | 37.5 % (3 of 8) |
Aromatic | 51.9 % (28 of 54) | 74.1 % (20 of 27) | 26.9 % (7 of 26) | 100.0 % (1 of 1) |
Methyl | 80.7 % (71 of 88) | 81.8 % (36 of 44) | 79.5 % (35 of 44) |
1. RST domain from the Radical-Induced Cell Death1
TTRPKSPWMP FPTLFAAISH KVAENDMLLI NADYQQLRDK KMTRAEFVRK LRVIVGDDLL RSTITTLQNQ PKSSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 200 uM [U-13C; U-15N] RCD1-RST, 200 uM DREB2A, 0.2 mg/mL sodium azide, 0.7 mM DSS, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DREB2A | natural abundance | 200 uM | |
2 | DSS | natural abundance | 0.7 mM | |
3 | RCD1-RST | [U-13C; U-15N] | 200 uM | |
4 | sodium azide | natural abundance | 0.2 mg/mL | |
5 | NaCl | natural abundance | 100 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 150 uM [U-15N] RCD1-RST, 150 uM DREB2A, 0.2 mg/mL sodium azide, 0.7 mM DSS, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | DREB2A | natural abundance | 150 uM | |
7 | DSS | natural abundance | 0.7 mM | |
8 | RCD1-RST | [U-15N] | 150 uM | |
9 | sodium azide | natural abundance | 0.2 mg/mL | |
10 | NaCl | natural abundance | 100 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 200 uM [U-13C; U-15N] RCD1-RST, 200 uM [U-13C; U-15N] DREB2A, 0.2 mg/mL sodium azide, 0.7 mM DSS, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | DREB2A | [U-13C; U-15N] | 200 uM | |
12 | DSS | natural abundance | 0.7 mM | |
13 | RCD1-RST | [U-13C; U-15N] | 200 uM | |
14 | sodium azide | natural abundance | 0.2 mg/mL | |
15 | NaCl | natural abundance | 100 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 300 uM [U-15N] RCD1-RST, 900 uM DREB2A, 50 mg/mL C12E5, 0.2 mg/mL sodium azide, 0.7 mM DSS, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
16 | C12E5 | natural abundance | 50 mg/mL | |
17 | DREB2A | natural abundance | 900 uM | |
18 | DSS | natural abundance | 0.7 mM | |
19 | RCD1-RST | [U-15N] | 300 uM | |
20 | sodium azide | natural abundance | 0.2 mg/mL | |
21 | NaCl | natural abundance | 100 mM |