BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	76.4 % (146 of 191)	82.7 % (81 of 98)	67.6 % (50 of 74)	78.9 % (15 of 19)
Backbone	70.4 % (76 of 108)	81.1 % (30 of 37)	60.4 % (32 of 53)	77.8 % (14 of 18)
Sidechain	85.0 % (85 of 100)	83.6 % (51 of 61)	86.8 % (33 of 38)	100.0 % (1 of 1)
Aromatic	60.0 % (6 of 10)	60.0 % (3 of 5)	60.0 % (3 of 5)
Methyl	81.3 % (13 of 16)	75.0 % (6 of 8)	87.5 % (7 of 8)

1. DREB2A

SSDMFDVDEL LRDLNGDD

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 240 uM [U-13C; U-15N] DREB2A, 240 uM RCD1-RST, 0.2 mg/mL sodium azide, 0.7 mM DSS, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	DREB2A	[U-13C; U-15N]	240 uM
2	DSS	natural abundance	0.7 mM
3	RCD1-RST	natural abundance	240 uM
4	sodium azide	natural abundance	0.2 mg/mL
5	sodium chloride	natural abundance	100 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 200 uM [U-15N] DREB2A, 200 uM RCD1-RST, 0.2 mg/mL sodium azide, 0.7 mM DSS, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
6	DREB2A	[U-15N]	200 uM
7	DSS	natural abundance	0.7 mM
8	RCD1-RST	natural abundance	200 uM
9	sodium azide	natural abundance	0.2 mg/mL
10	sodium chloride	natural abundance	100 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 5OAP, Strand ID: A Detail

Release date

2017-06-26

Citation

Connecting the αα-hubs: same fold, disordered ligands, new functions
Bugge, K., Staby, L., Kemplen, K.R., O'Shea, C., Bendsen, S.K., Jensen, M.K., Olsen, J.G., Skriver, K., Kragelund, B.B.
Cell Commun. Signal. (2021), 19, 2-2, PubMed 33407551 , DOI 10.1186/s12964-020-00686-8 , Abstract

Related entities 1. Bound DREB2A(255-272), : 1 : 2 : 1 entities Detail

Interaction partners 1. Bound DREB2A(255-272), : 4 interactors Detail

More details for 4 interactors identified by 3 citations

Experiments performed 11 experiments Detail

1 3D HNHA

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 200 uM [U-15N] DREB2A, 200 uM RCD1-RST, 0.2 mg/mL sodium azide, 0.7 mM DSS, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
6	DREB2A	[U-15N]	200 uM
7	DSS	natural abundance	0.7 mM
8	RCD1-RST	natural abundance	200 uM
9	sodium azide	natural abundance	0.2 mg/mL
10	sodium chloride	natural abundance	100 mM

2 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 240 uM [U-13C; U-15N] DREB2A, 240 uM RCD1-RST, 0.2 mg/mL sodium azide, 0.7 mM DSS, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	DREB2A	[U-13C; U-15N]	240 uM
2	DSS	natural abundance	0.7 mM
3	RCD1-RST	natural abundance	240 uM
4	sodium azide	natural abundance	0.2 mg/mL
5	sodium chloride	natural abundance	100 mM

3 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DREB2A	[U-13C; U-15N]	240 uM
2	DSS	natural abundance	0.7 mM
3	RCD1-RST	natural abundance	240 uM
4	sodium azide	natural abundance	0.2 mg/mL
5	sodium chloride	natural abundance	100 mM

4 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DREB2A	[U-13C; U-15N]	240 uM
2	DSS	natural abundance	0.7 mM
3	RCD1-RST	natural abundance	240 uM
4	sodium azide	natural abundance	0.2 mg/mL
5	sodium chloride	natural abundance	100 mM

5 2D 1H-15N HSQC

Instrument

Bruker Avance - 750 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DREB2A	[U-13C; U-15N]	240 uM
2	DSS	natural abundance	0.7 mM
3	RCD1-RST	natural abundance	240 uM
4	sodium azide	natural abundance	0.2 mg/mL
5	sodium chloride	natural abundance	100 mM

6 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 750 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DREB2A	[U-13C; U-15N]	240 uM
2	DSS	natural abundance	0.7 mM
3	RCD1-RST	natural abundance	240 uM
4	sodium azide	natural abundance	0.2 mg/mL
5	sodium chloride	natural abundance	100 mM

7 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 750 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DREB2A	[U-13C; U-15N]	240 uM
2	DSS	natural abundance	0.7 mM
3	RCD1-RST	natural abundance	240 uM
4	sodium azide	natural abundance	0.2 mg/mL
5	sodium chloride	natural abundance	100 mM

8 3D 1H-15N NOESY

Instrument

Varian INOVA - 750 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DREB2A	[U-13C; U-15N]	240 uM
2	DSS	natural abundance	0.7 mM
3	RCD1-RST	natural abundance	240 uM
4	sodium azide	natural abundance	0.2 mg/mL
5	sodium chloride	natural abundance	100 mM

9 3D 1H-13C NOESY aromatic

Instrument

Varian INOVA - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DREB2A	[U-13C; U-15N]	240 uM
2	DSS	natural abundance	0.7 mM
3	RCD1-RST	natural abundance	240 uM
4	sodium azide	natural abundance	0.2 mg/mL
5	sodium chloride	natural abundance	100 mM

10 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DREB2A	[U-13C; U-15N]	240 uM
2	DSS	natural abundance	0.7 mM
3	RCD1-RST	natural abundance	240 uM
4	sodium azide	natural abundance	0.2 mg/mL
5	sodium chloride	natural abundance	100 mM

11 3D HN(CA)CO

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DREB2A	[U-13C; U-15N]	240 uM
2	DSS	natural abundance	0.7 mM
3	RCD1-RST	natural abundance	240 uM
4	sodium azide	natural abundance	0.2 mg/mL
5	sodium chloride	natural abundance	100 mM

NMR combined restraints 4 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_34152_5oap.nef
Input source #2: Coordindates	5oap.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

---260-------270--
SSDMFDVDELLRDLNGDD
||||||||||||||||||
SSDMFDVDELLRDLNGDD
--------10--------

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	18	0	0	100.0

Content subtype: combined_34152_5oap.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	145		94.4 (chain: A, length: 18)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	Warning	287 (1)	noe	88.9 (chain: A, length: 18)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_3	OK	26 (26)	.	88.9 (chain: A, length: 18)

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 18, Sequence coverage: 94.4%
- Total number of assignments
  - ¹H chemical shifts: 81
  - ¹³C chemical shifts: 49
  - ¹⁵N chemical shifts: 15
- Completeness of assignments
  - Entity_assemble_ID: A
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	98	81	82.7
¹³C chemical shifts	74	49	66.2
¹⁵N chemical shifts	20	15	75.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	37	30	81.1
¹³C chemical shifts	36	16	44.4
¹⁵N chemical shifts	18	14	77.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	61	51	83.6
¹³C chemical shifts	38	33	86.8
¹⁵N chemical shifts	2	1	50.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	9	7	77.8
¹³C chemical shifts	9	8	88.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	5	3	60.0
¹³C chemical shifts	5	3	60.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 18, Sequence coverage: 88.9%
- Total number of restraints: 267
- Weight of restraints: 1.0 (267)
- Potential type of restraints: square-well-parabolic (267)
- Range of distance target values: 2.31, 4.63 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_3
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 18, Sequence coverage: 88.9%
- Total number of restraints: 26
- Total number of restraints per polymer type: 26
- Weight of restraints: 1.0 (26)
- Potential type of restraints: square-well-parabolic (26)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

Keywords IDP, TRANSCRIPTION, folding upon binding, plant protein, transcription factor

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Found 1 Document (0.629 seconds)

BMRB: 34152

Sample #1

Sample #2

Related entities 1. Bound DREB2A(255-272), : 1 : 2 : 1 entities Detail

Interaction partners 1. Bound DREB2A(255-272), : 4 interactors Detail

Experiments performed 11 experiments Detail

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NMR combined restraints 4 contents Detail