NMR structure of the complex formed by an engineered region 2 of sigmaE in complex with GTAAAA
MSEQLTDQVL VERVQKGDQK AFNLLVVRYQ HKVASLVSRY VPSGDVPDVV QEAFIKAYRA LDSFDINRKF STWLYRIAVN TAKNYLVAQG RRLEL
Polymer type: polypeptide(L) polydeoxyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 92.1 % (1103 of 1197) | 93.5 % (602 of 644) | 89.3 % (400 of 448) | 96.2 % (101 of 105) |
Backbone | 94.3 % (567 of 601) | 95.6 % (216 of 226) | 92.9 % (262 of 282) | 95.7 % (89 of 93) |
Sidechain | 90.7 % (624 of 688) | 92.3 % (386 of 418) | 87.6 % (226 of 258) | 100.0 % (12 of 12) |
Aromatic | 79.2 % (84 of 106) | 93.1 % (54 of 58) | 61.7 % (29 of 47) | 100.0 % (1 of 1) |
Methyl | 93.7 % (119 of 127) | 95.3 % (61 of 64) | 92.1 % (58 of 63) |
1. entity 1
MSEQLTDQVL VERVQKGDQK AFNLLVVRYQ HKVASLVSRY VPSGDVPDVV QEAFIKAYRA LDSFDINRKF STWLYRIAVN TAKNYLVAQG RRLEL2. entity 2
XTAAAASolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1 mM [U-99% 13C; U-99% 15N] Engineered region2 of sigmaE from E. coli in which the loop L3 was replaced by the loop L3 of Bacillus subtilis sigmaW, 1 mM DNA (5'-D(*GP*TP*AP*AP*AP*A)-3'), 10 mM sodium phosphate, 50 mM sodium chloride, 90% H2O/10% D2O.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DNA (5'-D(*GP*TP*AP*AP*AP*A)-3') | natural abundance | 1 mM | |
2 | engineered region 2 of sigmaE | [U-99% 13C; U-99% 15N] | 1 mM | |
3 | sodium chloride | natural abundance | 50 mM | |
4 | sodium phosphate | natural abundance | 10 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1 mM [U-99% 13C; U-99% 15N] Engineered region2 of sigmaE from E. coli in which the loop L3 was replaced by the loop L3 of Bacillus subtilis sigmaW, 1 mM DNA (5'-D(*GP*TP*AP*AP*AP*A)-3'), 10 mM sodium phosphate, 50 mM sodium chloride, 100% D2O.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | DNA (5'-D(*GP*TP*AP*AP*AP*A)-3') | natural abundance | 1 mM | |
6 | engineered region 2 of sigmaE | [U-99% 13C; U-99% 15N] | 1 mM | |
7 | sodium chloride | natural abundance | 50 mM | |
8 | sodium phosphate | natural abundance | 10 mM |