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BMRB: 34173

5OUN

NMR solution structure of the external DII domain of Rvb2 from Saccharomyces cerevisiae

Authors

Rouillon, C., Bragantini, B., Charpentier, B., Manival, X., Quinternet, M.

Assembly

RuvB-like protein 2 (E.C.3.6.4.12)

Entity

1. RuvB-like protein 2 (E.C.3.6.4.12) (polymer), 107 monomers, 11826.37 Da Detail

GPHMETELIE GEVVEIQIDR SITGGHKQGK LTIKTTDMET IYELGNKMID GLTKEKVLAG DVISIDKASG KITKLGRSFA RSRDYDAMGA DTRFVQCPEG ELQKRKT

Formula weight

11826.37 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 97.1 %, Completeness (bb): 97.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.1 % (1188 of 1224)	96.7 % (620 of 641)	97.0 % (459 of 473)	99.1 % (109 of 110)
Backbone	97.8 % (624 of 638)	96.0 % (215 of 224)	98.7 % (305 of 309)	99.0 % (104 of 105)
Sidechain	96.8 % (659 of 681)	97.1 % (405 of 417)	96.1 % (249 of 259)	100.0 % (5 of 5)
Aromatic	70.5 % (31 of 44)	86.4 % (19 of 22)	54.5 % (12 of 22)
Methyl	100.0 % (118 of 118)	100.0 % (59 of 59)	100.0 % (59 of 59)

1. entity 1

GPHMETELIE GEVVEIQIDR SITGGHKQGK LTIKTTDMET IYELGNKMID GLTKEKVLAG DVISIDKASG KITKLGRSFA RSRDYDAMGA DTRFVQCPEG ELQKRKT

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4, Details 1 mM [U-13C; U-15N] Rvb2DII, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	Rvb2DII	[U-13C; U-15N]		1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.18 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5OUN, Strand ID: A Detail

Release date

2017-08-24

Citation

NMR assignment and solution structure of the external DII domain of the yeast Rvb2 protein
Bragantini, B., Rouillon, C., Charpentier, B., Manival, X., Quinternet, M.
Biomol. NMR Assign. (2018), 12, 243-247, PubMed 29569106 , DOI 10.1007/s12104-018-9816-5 , Abstract

Related entities 1. RuvB-like protein 2 (E.C.3.6.4.12), : 1 : 2 : 86 entities Detail

Interaction partners 1. RuvB-like protein 2 (E.C.3.6.4.12), : 193 interactors Detail

More details for 193 interactors identified by 14 citations

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC