BMRB: 34187

Structure and dynamics conspire in the evolution of affinity between intrinsically disordered proteins

Authors

Chi, N.C.

Assembly

CID, NCBD

Entity

1. CID, NCBD, entity 1 (polymer, Thiol state: not present), 45 monomers, 4820.171 Da Detail

GSESQNDEKA LLDQLDSLLS STDEMELAEI DRALGIDKLV SQQGG

2. CID, NCBD, entity 2 (polymer, Thiol state: not present), 50 monomers, 5608.341 Da Detail

GSTPPQALQQ LLQTLKSPSS PQQQQQVLQI LKSNPQLMAA FIKQRSQHQQ

Total weight

10428.512 Da

Max. entity weight

5608.341 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 74.7 %, Completeness (bb): 81.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	74.7 % (825 of 1105)	83.8 % (491 of 586)	59.1 % (241 of 408)	83.8 % (93 of 111)
Backbone	81.3 % (455 of 560)	94.2 % (179 of 190)	67.5 % (189 of 280)	96.7 % (87 of 90)
Sidechain	72.3 % (459 of 635)	78.8 % (312 of 396)	64.7 % (141 of 218)	28.6 % (6 of 21)
Aromatic	0.0 % (0 of 14)	0.0 % (0 of 7)	0.0 % (0 of 7)
Methyl	78.4 % (80 of 102)	96.1 % (49 of 51)	60.8 % (31 of 51)

1. entity 1

GSESQNDEKA LLDQLDSLLS STDEMELAEI DRALGIDKLV SQQGG

2. entity 2

GSTPPQALQQ LLQTLKSPSS PQQQQQVLQI LKSNPQLMAA FIKQRSQHQQ

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 298 K, pH 6.5, Details 100 uM [U-100% 13C] 13C, 100 uM [U-100% 15N] 15N, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-100% 13C]		100 uM
2	entity_2	[U-100% 15N]		100 uM

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Calculated from 20 models in PDB: 6ES5, Strand ID: A, B Detail

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Release date

2019-03-18

Citation

Structure and dynamics conspire in the evolution of affinity between intrinsically disordered proteins
Jemth, P., Karlsson, E., Vogeli, B., Guzovsky, B., Andersson, E., Hultqvist, G., Dogan, J., Guntert, P., Riek, R., Chi, C.N.
Sci. Adv. (2018), 4, eaau4130-eaau4130, PubMed 30397651 , DOI 10.1126/sciadv.aau4130 , Abstract

Related entities 1. CID, NCBD, entity 1, : 1 : 1 : 35 entities Detail

Related entities 2. CID, NCBD, entity 2, : 1 : 1 : 34 entities Detail

Experiments performed 9 experiments Detail