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Structure and dynamics conspire in the evolution of affinity between intrinsically disordered proteins
Authors
Chi, N.C.
Assembly
CID, NCBD
Entity
1. CID, NCBD, entity 1 (polymer, Thiol state: not present), 45 monomers, 4820.171 Da Detail

GSESQNDEKA LLDQLDSLLS STDEMELAEI DRALGIDKLV SQQGG


2. CID, NCBD, entity 2 (polymer, Thiol state: not present), 50 monomers, 5608.341 Da Detail

GSTPPQALQQ LLQTLKSPSS PQQQQQVLQI LKSNPQLMAA FIKQRSQHQQ


Total weight
10428.512 Da
Max. entity weight
5608.341 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts, spectral_peak_list
Chem. Shift Complete
Sequence coverage: 98.9 %, Completeness: 74.7 %, Completeness (bb): 81.3 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All74.7 % (825 of 1105)83.8 % (491 of 586)59.1 % (241 of 408)83.8 % (93 of 111)
Backbone81.3 % (455 of 560)94.2 % (179 of 190)67.5 % (189 of 280)96.7 % (87 of 90)
Sidechain72.3 % (459 of 635)78.8 % (312 of 396)64.7 % (141 of 218)28.6 % (6 of 21)
Aromatic 0.0 % (0 of 14) 0.0 % (0 of 7) 0.0 % (0 of 7)
Methyl78.4 % (80 of 102)96.1 % (49 of 51)60.8 % (31 of 51)

1. entity 1

GSESQNDEKA LLDQLDSLLS STDEMELAEI DRALGIDKLV SQQGG

2. entity 2

GSTPPQALQQ LLQTLKSPSS PQQQQQVLQI LKSNPQLMAA FIKQRSQHQQ

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 298 K, pH 6.5, Details 100 uM [U-100% 13C] 13C, 100 uM [U-100% 15N] 15N, 90% H2O/10% D2O


#NameIsotope labelingTypeConcentration
1entity_1[U-100% 13C]100 uM
2entity_2[U-100% 15N]100 uM

Protein Blocks Logo
Calculated from 20 models in PDB: 6ES5, Strand ID: A, B Detail


Release date
2019-03-18
Citation
Structure and dynamics conspire in the evolution of affinity between intrinsically disordered proteins
Jemth, P., Karlsson, E., Vogeli, B., Guzovsky, B., Andersson, E., Hultqvist, G., Dogan, J., Guntert, P., Riek, R., Chi, C.N.
Sci. Adv. (2018), 4, eaau4130-eaau4130, PubMed 30397651 , DOI 10.1126/sciadv.aau4130 ,
Entries sharing articles BMRB: 2 entries Detail
  BMRB: 34188 released on 2018-10-22
    Title Structure and dynamics conspire in the evolution of affinity between intrinsically disordered proteins
  BMRB: 34189 released on 2018-10-22
    Title Structure and dynamics conspire in the evolution of affinity between intrinsically disordered proteins
Related entities 1. CID, NCBD, entity 1, : 1 : 1 : 35 entities Detail
Related entities 2. CID, NCBD, entity 2, : 1 : 1 : 34 entities Detail
Experiments performed 9 experiments Detail
nullKeywords CID, Complex, DNA BINDING PROTEIN, IDP, NCBD