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BMRB: 34191

6EVI

solution NMR structure of EB1 C terminus (191-260)

Authors

Barsukov, I.L., Almeida, T.B.

Assembly

Microtubule-associated protein RP/EB family member 1

Entity

1. Microtubule-associated protein RP/EB family member 1 (polymer), 70 monomers, 8042.918 × 2 Da Detail

DEAAELMQQV KVLKLTVEDL EKERDFYFGK LRNIELICQE NEGENDPVLQ RIVDILYATD EGFVIPDEGG

Total weight

16085.836 Da

Max. entity weight

8042.918 Da

Source organism

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.3 %, Completeness (bb): 98.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.3 % (788 of 827)	92.6 % (400 of 432)	98.1 % (314 of 320)	98.7 % (74 of 75)
Backbone	98.1 % (408 of 416)	97.2 % (139 of 143)	98.5 % (202 of 205)	98.5 % (67 of 68)
Sidechain	93.5 % (445 of 476)	90.3 % (261 of 289)	98.3 % (177 of 180)	100.0 % (7 of 7)
Aromatic	100.0 % (46 of 46)	100.0 % (23 of 23)	100.0 % (23 of 23)
Methyl	100.0 % (86 of 86)	100.0 % (43 of 43)	100.0 % (43 of 43)

1. entity 1

DEAAELMQQV KVLKLTVEDL EKERDFYFGK LRNIELICQE NEGENDPVLQ RIVDILYATD EGFVIPDEGG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-13C; U-15N] End binding protein 1 (EB1), 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	End binding protein 1 (EB1)	[U-13C; U-15N]		1 mM

LACS Plot; CA

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: 0.11 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6EVI, Strand ID: A, B Detail

Release date

2019-03-26

Citation

Targeting SxIP-EB1 interaction: An integrated approach to the discovery of small molecule modulators of dynamic binding sites
Almeida, T.B., Carnell, A.J., Barsukov, I.L., Berry, N.G.
Sci. Rep. (2017), 7, 15533-15533, PubMed 29138501 , DOI 10.1038/s41598-017-15502-6 , Abstract

Related entities 1. Microtubule-associated protein RP/EB family member 1, : 1 : 6 : 13 : 58 entities Detail

Interaction partners 1. Microtubule-associated protein RP/EB family member 1, : 16 interactors Detail

More details for 16 interactors identified by 8 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-13C; U-15N] End binding protein 1 (EB1), 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	End binding protein 1 (EB1)	[U-13C; U-15N]		1 mM

2 2D 1H-13C HSQC aliphatic

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-13C; U-15N] End binding protein 1 (EB1), 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	End binding protein 1 (EB1)	[U-13C; U-15N]		1 mM

3 2D 1H-13C HSQC aromatic

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-13C; U-15N] End binding protein 1 (EB1), 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	End binding protein 1 (EB1)	[U-13C; U-15N]		1 mM

4 3D HNCO

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-13C; U-15N] End binding protein 1 (EB1), 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	End binding protein 1 (EB1)	[U-13C; U-15N]		1 mM

5 3D HNCA

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-13C; U-15N] End binding protein 1 (EB1), 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	End binding protein 1 (EB1)	[U-13C; U-15N]		1 mM

6 3D CBCA(CO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-13C; U-15N] End binding protein 1 (EB1), 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	End binding protein 1 (EB1)	[U-13C; U-15N]		1 mM

7 3D HNCACB

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-13C; U-15N] End binding protein 1 (EB1), 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	End binding protein 1 (EB1)	[U-13C; U-15N]		1 mM

8 3D HBHA(CO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-13C; U-15N] End binding protein 1 (EB1), 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	End binding protein 1 (EB1)	[U-13C; U-15N]		1 mM

9 3D 1H-13C NOESY aliphatic

Instrument

Bruker AvanceIII - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-13C; U-15N] End binding protein 1 (EB1), 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	End binding protein 1 (EB1)	[U-13C; U-15N]		1 mM

10 3D HCCH-TOCSY

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-13C; U-15N] End binding protein 1 (EB1), 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	End binding protein 1 (EB1)	[U-13C; U-15N]		1 mM

11 3D 1H-13C NOESY aromatic

Instrument

Bruker AvanceIII - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-13C; U-15N] End binding protein 1 (EB1), 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	End binding protein 1 (EB1)	[U-13C; U-15N]		1 mM

12 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-13C; U-15N] End binding protein 1 (EB1), 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	End binding protein 1 (EB1)	[U-13C; U-15N]		1 mM

nullKeywords APC-binding protein EB1, EB1, End-binding protein, MAPRE1, Microtubule-associated protein RP/EB family member 1, PROTEIN BINDING, SxIP, microtubules