BMRB: 34199

Specific phosphorothioate substitution within domain 6 of a group II intron ribozyme leads to changes in local structure and metal ion binding

Authors

Erat, M.C., Besic, E., Oberhuber, M., Johannsen, S., Sigel, R.K.O.

Assembly

RNA (27-MER)

Entity

1. RNA (27-MER) (polymer, Thiol state: not present), 27 monomers, 8863.532 Da Detail

GGAGCGGGGG XGXAAACCXA XCGCXCC

Formula weight

8863.532 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 81.5 %, Completeness: 85.8 %, Completeness (bb): 97.7 % Detail

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Polymer type: polyribonucleotide

	Total	1H
All	85.8 % (163 of 190)	85.8 % (163 of 190)
Suger, PO4	97.7 % (129 of 132)	97.7 % (129 of 132)
Nucleobase	58.6 % (34 of 58)	58.6 % (34 of 58)
Aromatic	77.3 % (34 of 44)	77.3 % (34 of 44)

1. entity 1

GGAGCGGGGG XGXAAACCXA XCGCXCC

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.7 (±0.1), Details 2 mM alpha-thio-D6-27, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	alpha-thio-D6-27	natural abundance		2 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.7 (±0.1), Details 0.7 mM na alpha-thio-D6-27, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
2	alpha-thio-D6-27	natural abundance		0.7 mM

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Release date

2018-01-10

Citation 1

Solution structure of domain 6 from a self-splicing group II intron ribozyme: a Mg(2+) binding site is located close to the stacked branch adenosine
Erat, M.C., Zerbe, O., Fox, T., Sigel, R.K.
Chembiochem. (2007), 8, 306-314, PubMed 17200997 , DOI 10.1002/cbic.200600459 , Abstract

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Citation 2

Specific phosphorothioate substitution within domain 6 of a group II intron ribozyme leads to changes in local structure and metal ion binding
Erat, M.C., Besic, E., Oberhuber, M., Johannsen, S., Sigel, R.K.O., Erat, M.C., Zerbe, O., Fox, T., Sigel, R.K.
J. Biol. Inorg. Chem. (2017), 23, 167-177, PubMed 29218637 , DOI 10.1007/s00775-017-1519-3 , Abstract

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Experiments performed 5 experiments Detail