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NMR structure of the second TPR domain of the human RPAP3 protein in complex with HSP70 peptide SGPTIEEVD
Authors
Quinternet, M., Chagot, M.E., Manival, X.
Assembly
RNA polymerase II-associated protein 3, Heat shock 70 kDa protein 1B
Entity
1. RNA polymerase II-associated protein 3, Heat shock 70 kDa protein 1B, entity 1 (polymer), 120 monomers, 13346.10 Da Detail

GPHMQAISEK DRGNGFFKEG KYERAIECYT RGIAADGANA LLPANRAMAY LKIQKYEEAE KDCTQAILLD GSYSKAFARR GTARTFLGKL NEAKQDFETV LLLEPGNKQA VTELSKIKKK


2. RNA polymerase II-associated protein 3, Heat shock 70 kDa protein 1B, entity 2 (polymer, Thiol state: not present), 9 monomers, 945.9668 Da Detail

SGPTIEEVD


Total weight
14292.066 Da
Max. entity weight
13346.1 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 93.2 %, Completeness (bb): 94.3 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All93.2 % (1395 of 1497)95.9 % (754 of 786)88.9 % (512 of 576)95.6 % (129 of 135)
Backbone94.3 % (722 of 766)96.2 % (255 of 265)92.6 % (348 of 376)95.2 % (119 of 125)
Sidechain92.3 % (784 of 849)95.8 % (499 of 521)86.5 % (275 of 318)100.0 % (10 of 10)
Aromatic58.5 % (55 of 94)74.5 % (35 of 47)42.6 % (20 of 47)
Methyl96.9 % (126 of 130)100.0 % (65 of 65)93.8 % (61 of 65)

1. entity 1

GPHMQAISEK DRGNGFFKEG KYERAIECYT RGIAADGANA LLPANRAMAY LKIQKYEEAE KDCTQAILLD GSYSKAFARR GTARTFLGKL NEAKQDFETV LLLEPGNKQA VTELSKIKKK

2. entity 2

SGPTIEEVD

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.4, Details 1 mM [U-13C; U-15N] RPAP3-TPR2, 1 mM HSP70-pep, 150 mM sodium chloride, 10 mM sodium phosphate, 0.5 mM TCEP, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
1RPAP3-TPR2[U-13C; U-15N]1 mM
2HSP70-pepnatural abundance1 mM
3sodium chloridenatural abundance150 mM
4sodium phosphatenatural abundance10 mM
5TCEPnatural abundance0.5 mM
Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.4, Details 1 mM [U-13C; U-15N] RPAP3-TPR2, 1 mM HSP70-pep, 150 mM sodium chloride, 10 mM sodium phosphate, 0.5 mM TCEP, 100% D2O


#NameIsotope labelingTypeConcentration
6RPAP3-TPR2[U-13C; U-15N]1 mM
7HSP70-pepnatural abundance1 mM
8sodium chloridenatural abundance150 mM
9sodium phosphatenatural abundance10 mM
10TCEPnatural abundance0.5 mM

Release date
2018-01-09
Citation
Deep Structural Analysis of RPAP3 and PIH1D1, Two Components of the HSP90 Co-chaperone R2TP Complex
Henri, J., Chagot, M.E., Bourguet, M., Abel, Y., Terral, G., Maurizy, C., Aigueperse, C., Georgescauld, F., Vandermoere, F., Saint-Fort, R., Behm-Ansmant, I., Charpentier, B., Pradet-Balade, B., Verheggen, C., Bertrand, E., Meyer, P., Cianferani, S., Manival, X., Quinternet, M.
Structure (2018), 26, 1196-1209, PubMed 30033218 , DOI 10.1016/j.str.2018.06.002 ,
Related entities 1. RNA polymerase II-associated protein 3, Heat shock 70 kDa protein 1B, entity 1, : 1 : 125 entities Detail
Experiments performed 16 experiments Detail
NMR combined restraints 4 contents Detail
Keywords TPR HSP RUVBL Polymerase, chaperone