BMRB: 34249

FLN5 (full length)

Authors

Waudby, C.A., Wlodarski, T., Karyadi, M.E., Cassaignau, A.M.E., Chan, S.H.S., Wentink, A.S., Schmidt-Engler, J.M., Camilloni, C., Vendruscolo, M., Cabrita, L.D., Christodoulou, J.

Assembly

Gelation factor

Entity

1. Gelation factor (polymer), 114 monomers, 12152.25 Da Detail

MHHHHHHASK PAPSAEHSYA EGEGLVKVFD NAPAEFTIFA VDTKGVARTD GGDPFEVAIN GPDGLVVDAK VTDNNDGTYG VVYDAPVEGN YNVNVTLRGN PIKNMPIDVK CIEG

Formula weight

12152.25 Da

Source organism

Dictyostelium discoideum

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.0 %, Completeness: 48.2 %, Completeness (bb): 85.0 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	48.2 % (593 of 1231)	34.5 % (217 of 629)	57.5 % (280 of 487)	83.5 % (96 of 115)
Backbone	85.0 % (568 of 668)	86.6 % (201 of 232)	82.1 % (271 of 330)	90.6 % (96 of 106)
Sidechain	16.4 % (109 of 665)	4.0 % (16 of 397)	35.9 % (93 of 259)	0.0 % (0 of 9)
Aromatic	8.0 % (8 of 100)	8.0 % (4 of 50)	8.0 % (4 of 50)
Methyl	16.4 % (20 of 122)	11.5 % (7 of 61)	21.3 % (13 of 61)

1. entity 1

MHHHHHHASK PAPSAEHSYA EGEGLVKVFD NAPAEFTIFA VDTKGVARTD GGDPFEVAIN GPDGLVVDAK VTDNNDGTYG VVYDAPVEGN YNVNVTLRGN PIKNMPIDVK CIEG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 7.5, Details 200 uM [U-13C; U-15N] FLN5, 10 mM HEPES, 30 mM ammonium chloride, 12 mM magnesium chloride, 2 mM beta-mercaptoethanol, 1 mM EDTA, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	FLN5	[U-13C; U-15N]		200 uM
2	HEPES	natural abundance		10 mM
3	ammonium chloride	natural abundance		30 mM
4	magnesium chloride	natural abundance		12 mM
5	beta-mercaptoethanol	natural abundance		2 mM
6	EDTA	natural abundance		1 mM

Hide sample codition

LACS Plot; CA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.96 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 10 models in PDB: 6G4A, Strand ID: A Detail

---

Release date

2019-03-31

Citation

Mapping energy landscapes of a growing filamin domain reveals an intermediate associated with proline isomerization during biosynthesis
Waudby, C.A., Wlodarski, T., Karyadi, M.E., Cassaignau, A.M.E., Chan, S.H.S., Wentink, A.S., Schmidt-Engler, J.M., Camilloni, C., Vendruscolo, M., Cabrita, L.D., Christodoulou, J.

Related entities 1. Gelation factor, : 1 : 2 : 5 : 156 entities Detail

Experiments performed 4 experiments Detail