Solution structure of the r(UGGUGGU)4 RNA quadruplex
Polymer type: polyribonucleotide
Total | 1H | 13C | 31P | |
---|---|---|---|---|
All | 91.9 % (102 of 111) | 86.4 % (51 of 59) | 97.8 % (44 of 45) | 100.0 % (7 of 7) |
Suger, PO4 | 98.8 % (83 of 84) | 97.6 % (41 of 42) | 100.0 % (35 of 35) | 100.0 % (7 of 7) |
Nucleobase | 70.4 % (19 of 27) | 58.8 % (10 of 17) | 90.0 % (9 of 10) | |
Aromatic | 70.4 % (19 of 27) | 58.8 % (10 of 17) | 90.0 % (9 of 10) |
1. entity 1
UGGUGGUSolvent system 100% D2O, Pressure 1 bar, Temperature 333 K, pH 6.8, Details 2.0 mM pUGGUGGU, 10 mM phosphate buffer, 50 mM potassium chloride, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | pUGGUGGU | natural abundance | 2.0 mM | |
2 | phosphate buffer | natural abundance | 10 mM | |
3 | potassium chloride | natural abundance | 50 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 333 K, pH 6.8, Details 2.0 mM pUGGUGGU, 10 mM phosphate buffer, 0.05 mM potassium chloride, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | pUGGUGGU | natural abundance | 2.0 mM | |
5 | phosphate buffer | natural abundance | 10 mM | |
6 | potassium chloride | natural abundance | 0.05 mM |
Polymer type: polyribonucleotide
Total | 1H | 13C | 31P | |
---|---|---|---|---|
All | 68.9 % (153 of 222) | 86.4 % (102 of 118) | 48.9 % (44 of 90) | 50.0 % (7 of 14) |
Suger, PO4 | 73.8 % (124 of 168) | 97.6 % (82 of 84) | 50.0 % (35 of 70) | 50.0 % (7 of 14) |
Nucleobase | 53.7 % (29 of 54) | 58.8 % (20 of 34) | 45.0 % (9 of 20) | |
Aromatic | 53.7 % (29 of 54) | 58.8 % (20 of 34) | 45.0 % (9 of 20) |
1. entity 1
UGGUGGUSolvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 2.0 mM pUGGUGGU, 10 mM phosphate buffer, 50 mM potassium chloride, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | pUGGUGGU | natural abundance | 2.0 mM | |
2 | phosphate buffer | natural abundance | 10 mM | |
3 | potassium chloride | natural abundance | 50 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 2.0 mM pUGGUGGU, 10 mM phosphate buffer, 0.05 mM potassium chloride, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | pUGGUGGU | natural abundance | 2.0 mM | |
5 | phosphate buffer | natural abundance | 10 mM | |
6 | potassium chloride | natural abundance | 0.05 mM |
Polymer type: polyribonucleotide
Total | 1H | 13C | 31P | |
---|---|---|---|---|
All | 62.8 % (209 of 333) | 89.3 % (158 of 177) | 32.6 % (44 of 135) | 33.3 % (7 of 21) |
Suger, PO4 | 64.7 % (163 of 252) | 96.0 % (121 of 126) | 33.3 % (35 of 105) | 33.3 % (7 of 21) |
Nucleobase | 56.8 % (46 of 81) | 72.5 % (37 of 51) | 30.0 % (9 of 30) | |
Aromatic | 56.8 % (46 of 81) | 72.5 % (37 of 51) | 30.0 % (9 of 30) |
1. entity 1
UGGUGGUSolvent system 100% D2O, Pressure 1 atm, Temperature 278 K, pH 6.8, Details 2.0 mM pUGGUGGU, 10 mM phosphate buffer, 50 mM potassium chloride, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | pUGGUGGU | natural abundance | 2.0 mM | |
2 | phosphate buffer | natural abundance | 10 mM | |
3 | potassium chloride | natural abundance | 50 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 6.8, Details 2.0 mM pUGGUGGU, 10 mM phosphate buffer, 0.05 mM potassium chloride, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | pUGGUGGU | natural abundance | 2.0 mM | |
5 | phosphate buffer | natural abundance | 10 mM | |
6 | potassium chloride | natural abundance | 0.05 mM |