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BMRB: 34280

6GPI

Tc-DNA/DNA duplex

Authors

Istrate, A., Johannsen, S., Istrate, A., Sigel, R.K.O., Leumann, C.

Assembly

Tc-DNA/DNA duplex

Entity

1. Tc-DNA/DNA duplex, entity 1 (polymer, Thiol state: not present), 10 monomers, 3415.546 Da
2. Tc-DNA/DNA duplex, entity 2 (polymer, Thiol state: not present), 10 monomers, 3157.014 Da Detail

GTAAGCCGAG

Total weight

6572.5596 Da

Max. entity weight

3415.546 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 50.0 %, Completeness: 97.9 %, Completeness (bb): 98.6 % Detail

Polymer type: polydeoxyribonucleotide

	Total	¹H
All	97.9 % (93 of 95)	97.9 % (93 of 95)
Suger, PO4	98.6 % (69 of 70)	98.6 % (69 of 70)
Nucleobase	96.0 % (24 of 25)	96.0 % (24 of 25)
Aromatic	95.0 % (19 of 20)	95.0 % (19 of 20)
Methyl	100.0 % (1 of 1)	100.0 % (1 of 1)

2. entity 2

GTAAGCCGAG

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.05, Details 0.5 mM tc-DNA/DNA, 50 mM NaCl, 10 mM NaH2PO4, 100% D2O

#	Name	Isotope labeling	Concentration
1	tc-DNA/DNA	natural abundance	0.5 mM
2	tc-DNA/DNA	natural abundance	0.5 mM
3	NaCl	natural abundance	50 mM
4	NaH2PO4	natural abundance	10 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.05, Details 0.5 mM tc-DNA/DNA, 50 mM NaCl, 10 mM NaH2PO4, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	tc-DNA/DNA	natural abundance	0.5 mM
6	tc-DNA/DNA	natural abundance	0.5 mM
7	NaCl	natural abundance	50 mM
8	NaH2PO4	natural abundance	10 mM

Release date

2018-06-06

Citation

NMR solution structure of tricyclo-DNA containing duplexes: insight into enhanced thermal stability and nuclease resistance
Istrate, A., Johannsen, S., Istrate, A., Sigel, R.K.O., Leumann, C.
Nucleic Acids Res. (2019), 47, 4872-4882, PubMed 30916334 , DOI 10.1093/nar/gkz197 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 34276 released on 2018-06-03
    Title Tc-DNA/RNA duplex
  BMRB: 34277 released on 2018-05-31
    Title tc-DNA/tc-DNA duplex

Experiments performed 6 experiments Detail

1 2D 1H-1H NOESY

Instrument

Bruker AvanceII - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.05, Details 0.5 mM tc-DNA/DNA, 50 mM NaCl, 10 mM NaH2PO4, 100% D2O

#	Name	Isotope labeling	Concentration
1	tc-DNA/DNA	natural abundance	0.5 mM
2	tc-DNA/DNA	natural abundance	0.5 mM
3	NaCl	natural abundance	50 mM
4	NaH2PO4	natural abundance	10 mM

2 2D 1H-1H NOESY

Instrument

Bruker AvanceII - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 283 K, pH 7.05, Details 0.5 mM tc-DNA/DNA, 50 mM NaCl, 10 mM NaH2PO4, 100% D2O

#	Name	Isotope labeling	Concentration
1	tc-DNA/DNA	natural abundance	0.5 mM
2	tc-DNA/DNA	natural abundance	0.5 mM
3	NaCl	natural abundance	50 mM
4	NaH2PO4	natural abundance	10 mM

3 2D 1H-1H NOESY

Instrument

Bruker AvanceII - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 7.05, Details 0.5 mM tc-DNA/DNA, 50 mM NaCl, 10 mM NaH2PO4, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	tc-DNA/DNA	natural abundance	0.5 mM
6	tc-DNA/DNA	natural abundance	0.5 mM
7	NaCl	natural abundance	50 mM
8	NaH2PO4	natural abundance	10 mM

4 2D 1H-1H TOCSY

Instrument

Bruker AvanceII - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.05, Details 0.5 mM tc-DNA/DNA, 50 mM NaCl, 10 mM NaH2PO4, 100% D2O

#	Name	Isotope labeling	Concentration
1	tc-DNA/DNA	natural abundance	0.5 mM
2	tc-DNA/DNA	natural abundance	0.5 mM
3	NaCl	natural abundance	50 mM
4	NaH2PO4	natural abundance	10 mM

5 2D DQF-COSY

Instrument

Bruker AvanceII - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.05, Details 0.5 mM tc-DNA/DNA, 50 mM NaCl, 10 mM NaH2PO4, 100% D2O

#	Name	Isotope labeling	Concentration
1	tc-DNA/DNA	natural abundance	0.5 mM
2	tc-DNA/DNA	natural abundance	0.5 mM
3	NaCl	natural abundance	50 mM
4	NaH2PO4	natural abundance	10 mM

6 2D 1H-13C HSQC

Instrument

Bruker AvanceII - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.05, Details 0.5 mM tc-DNA/DNA, 50 mM NaCl, 10 mM NaH2PO4, 100% D2O

#	Name	Isotope labeling	Concentration
1	tc-DNA/DNA	natural abundance	0.5 mM
2	tc-DNA/DNA	natural abundance	0.5 mM
3	NaCl	natural abundance	50 mM
4	NaH2PO4	natural abundance	10 mM

nullKeywords DNA-RNA HYBRID, Modified nucleic acids, Modified oligonucleotide, Tc-DNA, tricyclo-DNA

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Found 1 Document (0.541 seconds)

BMRB: 34280

Sample #1

Sample #2

Entries sharing articles BMRB: 2 entries Detail

Experiments performed 6 experiments Detail

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