Solution structure of the capsid domain from the activity-regulated cytoskeleton-associated protein, Arc
SPGLDTQIFE DPREFLSHLE EYLRQVGGSE EYWLSQIQNH MNGPAKKWWE FKQGSVKNWV EFKKEFLQYS EGTLSREAIQ RELDLPQKQG EPLDQFLWRK RDLYQTLYVD AEEEEIIQYV VGTLQPKFKR FLRHPLPKTL EQLIQRGMEV QDGLEQAAE
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 81.9 % (1639 of 2001) | 79.1 % (838 of 1060) | 84.4 % (646 of 765) | 88.1 % (155 of 176) |
Backbone | 95.8 % (899 of 938) | 93.8 % (300 of 320) | 96.6 % (451 of 467) | 98.0 % (148 of 151) |
Sidechain | 73.0 % (885 of 1212) | 72.7 % (538 of 740) | 76.1 % (340 of 447) | 28.0 % (7 of 25) |
Aromatic | 61.5 % (123 of 200) | 70.0 % (70 of 100) | 50.5 % (48 of 95) | 100.0 % (5 of 5) |
Methyl | 89.2 % (132 of 148) | 90.5 % (67 of 74) | 87.8 % (65 of 74) |
1. entity 1
SPGLDTQIFE DPREFLSHLE EYLRQVGGSE EYWLSQIQNH MNGPAKKWWE FKQGSVKNWV EFKKEFLQYS EGTLSREAIQ RELDLPQKQG EPLDQFLWRK RDLYQTLYVD AEEEEIIQYV VGTLQPKFKR FLRHPLPKTL EQLIQRGMEV QDGLEQAAESolvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 7.4, Details 0.5 mM [U-15N] Arc, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Arc | [U-15N] | 0.5 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 7.4, Details 1 mM [U-13C; U-15N] Arc, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | Arc | [U-13C; U-15N] | 1 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 7.4, Details 0.5 mM [U-15N] Arc, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | Arc | [U-15N] | 0.5 mM |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
1H | DSS | methyl protons | 0.0 ppm | internal | direct | 1.0 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
1H | DSS | methyl protons | 0.0 ppm | internal | direct | 1.0 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
1H | DSS | methyl protons | 0.0 ppm | internal | direct | 1.0 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
1H | DSS | methyl protons | 0.0 ppm | internal | direct | 1.0 |
Agilent DD2 - 800 MHz RT-probe
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 7.4, Details 1 mM [U-13C; U-15N] Arc, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | Arc | [U-13C; U-15N] | 1 mM |
Bruker AvanceIII - 750 MHz cryo-probe
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 7.4, Details 1 mM [U-13C; U-15N] Arc, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | Arc | [U-13C; U-15N] | 1 mM |
Bruker AvanceIII - 750 MHz cryo-probe
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 7.4, Details 1 mM [U-13C; U-15N] Arc, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | Arc | [U-13C; U-15N] | 1 mM |
Bruker AvanceIII - 750 MHz cryo-probe
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 7.4, Details 1 mM [U-13C; U-15N] Arc, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | Arc | [U-13C; U-15N] | 1 mM |
Bruker AvanceIII - 750 MHz cryo-probe
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 7.4, Details 1 mM [U-13C; U-15N] Arc, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | Arc | [U-13C; U-15N] | 1 mM |
Bruker AvanceIII - 750 MHz cryo-probe
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 7.4, Details 1 mM [U-13C; U-15N] Arc, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | Arc | [U-13C; U-15N] | 1 mM |
Bruker AvanceIII - 750 MHz cryo-probe
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 7.4, Details 1 mM [U-13C; U-15N] Arc, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | Arc | [U-13C; U-15N] | 1 mM |
Agilent DD2 - 800 MHz RT-probe
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 7.4, Details 1 mM [U-13C; U-15N] Arc, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | Arc | [U-13C; U-15N] | 1 mM |
Agilent DD2 - 800 MHz RT-probe
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 7.4, Details 1 mM [U-13C; U-15N] Arc, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | Arc | [U-13C; U-15N] | 1 mM |
Agilent DD2 - 800 MHz RT-probe
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 7.4, Details 1 mM [U-13C; U-15N] Arc, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | Arc | [U-13C; U-15N] | 1 mM |
Agilent DD2 - 800 MHz RT-probe
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 7.4, Details 1 mM [U-13C; U-15N] Arc, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | Arc | [U-13C; U-15N] | 1 mM |
Agilent DD2 - 800 MHz RT-probe
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 7.4, Details 1 mM [U-13C; U-15N] Arc, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | Arc | [U-13C; U-15N] | 1 mM |
Properties
Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints | combined_34285_6gse.nef |
Input source #2: Coordindates | 6gse.cif |
Diamagnetism of the molecular assembly | True (excluding Oxygen atoms) |
Whether the assembly has a disulfide bond | None |
Whether the assembly has a other bond | None |
Whether the assembly contains a cyclic polymer | None |
Overall data processing status | Warning |
Disulfide bonds
NoneOther bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)
NoneNon-standard residues
NoneSequence alignments
--210-------220-------230-------240-------250-------260-------270-------280-------290-------300----- SPGLDTQIFEDPREFLSHLEEYLRQVGGSEEYWLSQIQNHMNGPAKKWWEFKQGSVKNWVEFKKEFLQYSEGTLSREAIQRELDLPQKQGEPLDQFLWRK |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| SPGLDTQIFEDPREFLSHLEEYLRQVGGSEEYWLSQIQNHMNGPAKKWWEFKQGSVKNWVEFKKEFLQYSEGTLSREAIQRELDLPQKQGEPLDQFLWRK --------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 --310-------320-------330-------340-------350-------360---- RDLYQTLYVDAEEEEIIQYVVGTLQPKFKRFLRHPLPKTLEQLIQRGMEVQDGLEQAAE ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| RDLYQTLYVDAEEEEIIQYVVGTLQPKFKRFLRHPLPKTLEQLIQRGMEVQDGLEQAAE -------110-------120-------130-------140-------150---------
Chain assignments
Entity_assembly_ID (NMR) | Auth_asym_ID (model) | Length | Unmapped | Conflict | Sequence coverage (%) |
---|---|---|---|---|---|
A | A | 159 | 0 | 0 | 100.0 |
Content subtype: combined_34285_6gse.nef
Assigned chemical shifts
--210-------220-------230-------240-------250-------260-------270-------280-------290-------300----- SPGLDTQIFEDPREFLSHLEEYLRQVGGSEEYWLSQIQNHMNGPAKKWWEFKQGSVKNWVEFKKEFLQYSEGTLSREAIQRELDLPQKQGEPLDQFLWRK ||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||| .PGLDTQIFEDPREFLSHLEEYLRQVGGSEEYWLSQIQNHMN.PAKKWWEFKQGSVKNWVEFKKEFLQYSEGTLSREAIQRELDLPQKQGEPLDQFLWRK --310-------320-------330-------340-------350-------360---- RDLYQTLYVDAEEEEIIQYVVGTLQPKFKRFLRHPLPKTLEQLIQRGMEVQDGLEQAAE ||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||| RDLYQTLYVDAEEEEIIQYVVGTLQ.KFKRFLRHPLPKTLEQLIQRGMEVQDGLEQAAE
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 1060 | 785 | 74.1 |
13C chemical shifts | 765 | 615 | 80.4 |
15N chemical shifts | 185 | 150 | 81.1 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 320 | 298 | 93.1 |
13C chemical shifts | 318 | 304 | 95.6 |
15N chemical shifts | 151 | 145 | 96.0 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 740 | 487 | 65.8 |
13C chemical shifts | 447 | 311 | 69.6 |
15N chemical shifts | 34 | 5 | 14.7 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 76 | 66 | 86.8 |
13C chemical shifts | 76 | 61 | 80.3 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 100 | 56 | 56.0 |
13C chemical shifts | 95 | 37 | 38.9 |
15N chemical shifts | 5 | 5 | 100.0 |
Distance restraints
--210-------220-------230-------240-------250-------260-------270-------280-------290-------300----- SPGLDTQIFEDPREFLSHLEEYLRQVGGSEEYWLSQIQNHMNGPAKKWWEFKQGSVKNWVEFKKEFLQYSEGTLSREAIQRELDLPQKQGEPLDQFLWRK |||||| | |||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||| | |||| ||| ...LDTQIF.D.REFLSHLEEYLRQVGGSEEYWLSQIQNHMN..AKKWWEFKQGSVKNWVEFKKEFLQYSEGTLSREAIQRELDLPQK..E.LDQF.WRK --310-------320-------330-------340-------350-------360---- RDLYQTLYVDAEEEEIIQYVVGTLQPKFKRFLRHPLPKTLEQLIQRGMEVQDGLEQAAE ||||||||||||||||||||||||| |||||||||||||||||||||||||||||||| RDLYQTLYVDAEEEEIIQYVVGTLQ..FKRFLRHPLPKTLEQLIQRGMEVQDGLEQAAE
Dihedral angle restraints
--210-------220-------230-------240-------250-------260-------270-------280-------290-------300----- SPGLDTQIFEDPREFLSHLEEYLRQVGGSEEYWLSQIQNHMNGPAKKWWEFKQGSVKNWVEFKKEFLQYSEGTLSREAIQRELDLPQKQGEPLDQFLWRK ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| .........EDPREFLSHLEEYLRQVGGSEEYWLSQIQNHMNGPAKKWWEFKQGSVKNWVEFKKEFLQYSEGTLSREAIQRELDLPQKQGEPLDQFLWRK --210-------220-------230-------240-------250-------260-------270-------280-------290-------300----- --310-------320-------330-------340-------350-------360---- RDLYQTLYVDAEEEEIIQYVVGTLQPKFKRFLRHPLPKTLEQLIQRGMEVQDGLEQAAE ||||||||||||||||||||||||||||||||||||||||||||||||||||| RDLYQTLYVDAEEEEIIQYVVGTLQPKFKRFLRHPLPKTLEQLIQRGMEVQDG --310-------320-------330-------340-------350--------