B1-type ACP domain from module 7 of MLSB
GAASAATDLA ARLNGLSPQQ QQQTLATLVA AATATVLGHH TPESISPATA FKDLGIDSLT ALELRNTLTH NTGLDLPPTL IFDHPTPHAL TQHLHTRLTQ SH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 89.0 % (979 of 1100) | 86.5 % (481 of 556) | 92.0 % (404 of 439) | 89.5 % (94 of 105) |
Backbone | 95.2 % (569 of 598) | 94.6 % (191 of 202) | 95.3 % (287 of 301) | 95.8 % (91 of 95) |
Sidechain | 84.5 % (506 of 599) | 81.9 % (290 of 354) | 90.6 % (213 of 235) | 30.0 % (3 of 10) |
Aromatic | 69.2 % (36 of 52) | 69.2 % (18 of 26) | 69.2 % (18 of 26) | |
Methyl | 89.3 % (134 of 150) | 88.0 % (66 of 75) | 90.7 % (68 of 75) |
1. Type I modular polyketide synthase
GAASAATDLA ARLNGLSPQQ QQQTLATLVA AATATVLGHH TPESISPATA FKDLGIDSLT ALELRNTLTH NTGLDLPPTL IFDHPTPHAL TQHLHTRLTQ SHSolvent system 90% H2O/10% D2O, Pressure 1 (±.01) atm, Temperature 283 (±.5) K, pH 7.5 (±.05), Details 150 mM NA sodium choride, 50 mM NA sodium phosphate, 0.8 mM [U-15N] 15N_mH0ACPb, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | sodium choride | NA | 150 (±10.0) mM | |
2 | sodium phosphate | NA | 50 (±5.0) mM | |
3 | 15N_mH0ACPb | [U-15N] | 0.8 (±0.05) mM |
Solvent system 90% H2O/10% D2O, Pressure 1 (±.01) atm, Temperature 283 (±.5) K, pH 7.5 (±.05), Details 0.8 mM [U-13C; U-15N] 13C15N_mH0ACPb, 150 mM NA sodium chloride, 50 mM NA sodium phosphate, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | sodium phosphate | NA | 50 (±5.0) mM | |
5 | 13C15N_mH0ACPb | [U-13C; U-15N] | 0.8 (±0.05) mM | |
6 | sodium chloride | NA | 150 (±10.0) mM |