Yeast V-ATPase transmembrane helix 7 NMR structure in DPC micelles
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 47.0 % (143 of 304) | 78.5 % (124 of 158) | 12.6 % (15 of 119) | 14.8 % (4 of 27) |
Backbone | 41.3 % (62 of 150) | 96.0 % (48 of 50) | 13.3 % (10 of 75) | 16.0 % (4 of 25) |
Sidechain | 47.5 % (85 of 179) | 70.4 % (76 of 108) | 13.0 % (9 of 69) | 0.0 % (0 of 2) |
Aromatic | 46.4 % (13 of 28) | 92.9 % (13 of 14) | 0.0 % (0 of 13) | 0.0 % (0 of 1) |
Methyl | 60.0 % (18 of 30) | 100.0 % (15 of 15) | 20.0 % (3 of 15) |
1. entity 1
KKSHTASYLR LWALSLAHAQ LSSKKSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.0, Details 100 mM [U-99% 2H] Deuterated DPC, 50 mM potassium phosphate, 0.02 % sodium azide, 1 mM 13C and 15N labeled aminoacids 8 and 12 TM7 peptide, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Deuterated DPC | [U-99% 2H] | 100 mM | |
2 | potassium phosphate | none | 50 mM | |
3 | sodium azide | none | 0.02 % | |
4 | TM7 peptide | 13C and 15N labeled aminoacids 8 and 12 | 1 mM |