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BMRB: 34339

6QAN

Structure determination of N-terminal fragment of UL49.5 protein from bovine herpesvirus 1 by NMR spectroscopy and molecular dynamics

Authors

Karska, N., Rodziewicz-Motowidlo, S.

Assembly

Envelope glycoprotein N

Entity

1. Envelope glycoprotein N (polymer, Thiol state: not present), 36 monomers, 3821.282 Da Detail

RDPLLDAXRR EGAXDFWSAG XYARGVPLSE PPQALX

Formula weight

3821.282 Da

Source organism

Bovine alphaherpesvirus 1

Exptl. method

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.9 %, Completeness: 93.1 %, Completeness (bb): 98.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	93.1 % (175 of 188)	93.1 % (175 of 188)
Backbone	98.4 % (62 of 63)	98.4 % (62 of 63)
Sidechain	90.4 % (113 of 125)	90.4 % (113 of 125)
Aromatic	53.3 % (8 of 15)	53.3 % (8 of 15)
Methyl	100.0 % (15 of 15)	100.0 % (15 of 15)

1. Envelope glycoprotein N

RDPLLDAXRR EGAXDFWSAG XYARGVPLSE PPQALX

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 (±0.2) K, pH 6.5 (±0.1), Details 1.0 mM N.BHV, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	N.BHV	natural abundance		1.0 mM

Protein Blocks Logo

Calculated from 18 models in PDB: 6QAN, Strand ID: A Detail

Release date

2019-03-12

Citation

Structure determination of UL49.5 transmembrane protein from bovine herpesvirus 1 by NMR spectroscopy and molecular dynamics
Karska, N., Graul, M., Sikorska, E., Zhukov, I., Slusarz, M.J., Kasprzykowski, F., Lipinska, A.D., Rodziewicz-Motowidlo, S.
Biochim. Biophys. Acta. Biomembr. (2019), 1861, 926-938, PubMed 30772281 , DOI 10.1016/j.bbamem.2019.02.005 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 27730 released on 2018-12-16
    Title Structure determination of transmembrane- C-terminal fragment of UL49.5 protein from bovine herpesvirus 1 by NMR spectroscopy and molecular dynamics
  BMRB: 27728 released on 2018-12-13
    Title Structure determination of N-terminal fragment of UL49.5 protein from bovine herpesvirus 1 by NMR spectroscopy and molecular dynamics

Related entities 1. Envelope glycoprotein N, : 1 : 1 : 3 entities Detail

Interaction partners 1. Envelope glycoprotein N, : 6 interactors Detail

More details for 6 interactors identified by 1 citations

Experiments performed 9 experiments Detail

1 2D TOCSY

Instrument

Bruker AvanceIII - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 (±0.2) K, pH 6.5 (±0.1), Details 1.0 mM N.BHV, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	N.BHV	natural abundance		1.0 mM

2 2D TOCSY

Instrument

Varian Uniform NMR System - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 (±0.2) K, pH 6.5 (±0.1), Details 1.0 mM N.BHV, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	N.BHV	natural abundance		1.0 mM

3 2D NOESY

Instrument

Varian Uniform NMR System - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 (±0.2) K, pH 6.5 (±0.1), Details 1.0 mM N.BHV, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	N.BHV	natural abundance		1.0 mM

4 2D 1H-15N HSQC

Instrument

Varian Uniform NMR System - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 (±0.2) K, pH 6.5 (±0.1), Details 1.0 mM N.BHV, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	N.BHV	natural abundance		1.0 mM

5 2D 1H-13C HSQC aliphatic

Instrument

Varian Uniform NMR System - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 (±0.2) K, pH 6.5 (±0.1), Details 1.0 mM N.BHV, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	N.BHV	natural abundance		1.0 mM

6 2D 1H-13C HSQC aromatic

Instrument

Varian Uniform NMR System - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 (±0.2) K, pH 6.5 (±0.1), Details 1.0 mM N.BHV, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	N.BHV	natural abundance		1.0 mM

7 2D NOESY

Instrument

Bruker AvanceIII - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 (±0.2) K, pH 6.5 (±0.1), Details 1.0 mM N.BHV, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	N.BHV	natural abundance		1.0 mM

8 2D DQF-COSY

Instrument

Bruker AvanceIII - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 (±0.2) K, pH 6.5 (±0.1), Details 1.0 mM N.BHV, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	N.BHV	natural abundance		1.0 mM

9 2D ROESY

Instrument

Bruker AvanceIII - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 (±0.2) K, pH 6.5 (±0.1), Details 1.0 mM N.BHV, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	N.BHV	natural abundance		1.0 mM

nullKeywords MEMBRANE PROTEIN, NMR spectroscopy, herpesvirus, transmembrane protein