NMR structure of BB_P28, Borrelia burgdorferi outer surface lipoprotein
GAMGPKSKEE LLREKLSEDQ KTHLDWLKEA LGNDGEFDKF LGYDESKIKT ALDHIKSELD KCNGNDADQQ KTTFKQTVQG ALSGGIDGFG SNNAVTTCGN GS
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS62:SG | 1:CYS98:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 97.6 % (1112 of 1139) | 98.0 % (583 of 595) | 96.8 % (417 of 431) | 99.1 % (112 of 113) |
Backbone | 96.7 % (590 of 610) | 97.2 % (210 of 216) | 95.6 % (280 of 293) | 99.0 % (100 of 101) |
Sidechain | 98.7 % (610 of 618) | 98.4 % (373 of 379) | 99.1 % (225 of 227) | 100.0 % (12 of 12) |
Aromatic | 100.0 % (68 of 68) | 100.0 % (34 of 34) | 100.0 % (33 of 33) | 100.0 % (1 of 1) |
Methyl | 98.8 % (85 of 86) | 97.7 % (42 of 43) | 100.0 % (43 of 43) |
1. Surface protein, mlp lipoprotein family
GAMGPKSKEE LLREKLSEDQ KTHLDWLKEA LGNDGEFDKF LGYDESKIKT ALDHIKSELD KCNGNDADQQ KTTFKQTVQG ALSGGIDGFG SNNAVTTCGN GSSolvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.8 (±0.1), Details 20 mM sodium phosphate, 50 mM sodium chloride, 0.03 % w/v sodium azide, 1 mM EDTA, 2 mM U-C13, N15 BB_P28, 93% H2O/7% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | sodium phosphate | natural abundance | 20 (±1.0) mM | |
2 | sodium chloride | natural abundance | 50 (±1.0) mM | |
3 | sodium azide | natural abundance | 0.03 (±1.0E-4) % w/v | |
4 | EDTA | natural abundance | 1 (±0.01) mM | |
5 | BB_P28 | [U-13C; U-15N] | 2 (±0.1) mM |