BMRB: 34359

NMR solution structure of LSR2-T112D binding domain.

Authors

Barthe, P., Cohen-Gonsaud, M., Mukamolova, G.V.

Assembly

Nucleoid-associated protein Lsr2

Entity

1. Nucleoid-associated protein Lsr2 (polymer, Thiol state: not present), 51 monomers, 5496.930 Da Detail

GPHMSGSGRG RGAIDREQSA AIREWARRNG HNVSTRGRIP ADVIDAYHAA D

Formula weight

5496.93 Da

Source organism

Mycobacterium tuberculosis H37Rv

Exptl. method

solution NMR

Refine. method

MDSA and refinement in explicit solvent

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.1 %, Completeness: 92.2 %, Completeness (bb): 93.6 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	15N
All	92.2 % (307 of 333)	91.8 % (257 of 280)	94.3 % (50 of 53)
Backbone	93.6 % (146 of 156)	93.5 % (100 of 107)	93.9 % (46 of 49)
Sidechain	91.0 % (161 of 177)	90.8 % (157 of 173)	100.0 % (4 of 4)
Aromatic	88.2 % (15 of 17)	87.5 % (14 of 16)	100.0 % (1 of 1)
Methyl	100.0 % (21 of 21)	100.0 % (21 of 21)

1. entity 1

GPHMSGSGRG RGAIDREQSA AIREWARRNG HNVSTRGRIP ADVIDAYHAA D

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8, Details 0.5 mM [U-15N] LSR2-T112D protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	LSR2-T112D protein	[U-15N]		0.5 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8, Details 0.5 mM [U-15N] LSR2-T112D protein, 100% D2O

#	Name	Isotope labeling	Type	Concentration
2	LSR2-T112D protein	[U-15N]		0.5 mM

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 6QKQ, Strand ID: A Detail

---

Release date

2019-09-26

Citation

Protein kinase B controls Mycobacterium tuberculosis growth via phosphorylation of the transcriptional regulator Lsr2 at threonine 112
Alqaseer, K., Turapov, O., Barthe, P., Jagatia, H., De Visch, A., Roumestand, C., Wegrzyn, M., Bartek, I.L., Voskuil, M.I., O'Hare, H.M., Ajuh, P., Bottrill, A.R., Witney, A.A., Cohen-Gonsaud, M., Waddell, S.J., Mukamolova, G.V.
Mol. Microbiol. (2019), 112, 1847-1862, PubMed 31562654 , DOI 10.1111/mmi.14398 , Abstract

Related entities 1. Nucleoid-associated protein Lsr2, : 1 : 2 : 9 entities Detail

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail