BMRB: 34386

SC14 G-hairpin

Authors

Lenarcic Zivkovic, M., Trantirek, L., Plavec, J.

Assembly

DNA (5'-D(*GP*TP*GP*TP*GP*TP*GP*GP*GP*TP*GP*TP*GP*T)-3')

Entity

1. DNA (5'-D(*GP*TP*GP*TP*GP*TP*GP*GP*GP*TP*GP*TP*GP*T)-3') (polymer, Thiol state: not present), 14 monomers, 4476.817 Da Detail

GTGTGTGGGT GTGT

Formula weight

4476.817 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 87.1 %, Completeness (bb): 89.8 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	87.1 % (115 of 132)	87.1 % (115 of 132)
Suger, PO4	89.8 % (88 of 98)	89.8 % (88 of 98)
Nucleobase	79.4 % (27 of 34)	79.4 % (27 of 34)
Aromatic	75.0 % (21 of 28)	75.0 % (21 of 28)
Methyl	100.0 % (6 of 6)	100.0 % (6 of 6)

1. entity 1

GTGTGTGGGT GTGT

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 7.0, Details 0.6 mM / DNA (5'-D(*GP*TP*GP*TP*GP*TP*GP*GP*GP*TP*GP*TP*GP*T)-3'), 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	DNA (5'-D(*GP*TP*GP*TP*GP*TP*GP*GP*GP*TP*GP*TP*GP*T)-3')	natural abundance		0.6 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 283 K, pH 7.0, Details 0.6 mM / DNA (5'-D(*GP*TP*GP*TP*GP*TP*GP*GP*GP*TP*GP*TP*GP*T)-3'), 100% D2O

#	Name	Isotope labeling	Type	Concentration
2	DNA (5'-D(*GP*TP*GP*TP*GP*TP*GP*GP*GP*TP*GP*TP*GP*T)-3')	natural abundance		0.6 mM

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Release date

2020-03-02

Citation

Insight into formation propensity of pseudocircular DNA G-hairpins
Zdivkovic, M.L., Gajarsky, M., Bekova, K., Stadlbauer, P., Vicherek, L., Petrova, M., Fiala, R., Rosenberg, I., Sponer, J., Plavec, J., Trantirek, L.
Nucleic Acids Res. (2021), 49, 2317-2332, PubMed 33524154 , DOI 10.1093/nar/gkab029 , Abstract

Experiments performed 7 experiments Detail

Chemical shift validation 3 contents Detail