Extremely stable monomeric variant of human cystatin C with single amino acid substitution
SSPGKPPRLV GGPMDASVEE EGVRRALDFA VGEYNKASND MYHSRALQVV RARKQIGAGV NYFLDVELGR TTCTKTQPNL DNCPFHDQPH LKRKAFCSFQ IYAVPWQGTM TLSKSTCQDA
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS73:SG | 1:CYS83:SG |
2 | disulfide | sing | 1:CYS97:SG | 1:CYS117:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 84.7 % (1161 of 1370) | 84.8 % (607 of 716) | 85.6 % (453 of 529) | 80.8 % (101 of 125) |
Backbone | 93.3 % (657 of 704) | 93.8 % (226 of 241) | 94.0 % (330 of 351) | 90.2 % (101 of 112) |
Sidechain | 79.0 % (614 of 777) | 80.2 % (381 of 475) | 80.6 % (233 of 289) | 0.0 % (0 of 13) |
Aromatic | 0.0 % (0 of 106) | 0.0 % (0 of 53) | 0.0 % (0 of 52) | 0.0 % (0 of 1) |
Methyl | 99.1 % (109 of 110) | 98.2 % (54 of 55) | 100.0 % (55 of 55) |
1. entity 1
SSPGKPPRLV GGPMDASVEE EGVRRALDFA VGEYNKASND MYHSRALQVV RARKQIGAGV NYFLDVELGR TTCTKTQPNL DNCPFHDQPH LKRKAFCSFQ IYAVPWQGTM TLSKSTCQDASolvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 298 K, pH 7.4 (±0.1), Details 0.5 mM [U-99% 15N] hCC V57G, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | hCC V57G | [U-99% 15N] | 0.5 mM | |
2 | phosphate buffer | natural abundance | 50 mM | |
3 | NaCl | natural abundance | 50 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 298 K, pH 7.4 (±0.1), Details 0.5 mM [U-99% 13C; U-99% 15N] hCC V57G, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | hCC V57G | [U-99% 13C; U-99% 15N] | 0.5 mM | |
5 | phosphate buffer | natural abundance | 50 mM | |
6 | NaCl | natural abundance | 50 mM |