NMR structure of repeat domain 13 of the fibrillar adhesin CshA from Streptococcus gordonii.
MAHHHHHHSS GLEVLFQGPT GTGATSTGPQ GLPQTGTPTF QGGDPLVPID ETVEPTFEDG SKEKTIPGQG TYTIVPDGTV TFTPDKQFVG KPDPVTVKRV DKNGTPVTAT YSPEFTKV
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 63.4 % (820 of 1294) | 64.1 % (427 of 666) | 62.5 % (323 of 517) | 63.1 % (70 of 111) |
Backbone | 64.1 % (436 of 680) | 65.4 % (155 of 237) | 63.1 % (214 of 339) | 64.4 % (67 of 104) |
Sidechain | 62.9 % (451 of 717) | 63.4 % (272 of 429) | 62.6 % (176 of 281) | 42.9 % (3 of 7) |
Aromatic | 44.0 % (44 of 100) | 46.0 % (23 of 50) | 42.0 % (21 of 50) | |
Methyl | 74.6 % (88 of 118) | 76.3 % (45 of 59) | 72.9 % (43 of 59) |
1. Surface-associated protein CshA
MAHHHHHHSS GLEVLFQGPT GTGATSTGPQ GLPQTGTPTF QGGDPLVPID ETVEPTFEDG SKEKTIPGQG TYTIVPDGTV TFTPDKQFVG KPDPVTVKRV DKNGTPVTAT YSPEFTKVSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.5, Details 20 mg/mL [U-15N] CshA_RD13, 50 mM sodium chloride, 20 mM potassium phosphate, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CshA_RD13 | [U-15N] | 20 mg/mL | |
2 | sodium chloride | natural abundance | 50 mM | |
3 | potassium phosphate | natural abundance | 20 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.5, Details 20 mg/mL [U-13C; U-15N] CshA_RD13, 50 mM sodium chloride, 20 mM potassium phosphate, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | CshA_RD13 | [U-13C; U-15N] | 20 mg/mL | |
5 | sodium chloride | natural abundance | 50 mM | |
6 | potassium phosphate | natural abundance | 20 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7.5, Details 20 mg/mL [U-15N] CshA_RD13, 50 mM sodium chloride, 20 mM potassium phosphate, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | CshA_RD13 | [U-15N] | 20 mg/mL | |
8 | sodium chloride | natural abundance | 50 mM | |
9 | potassium phosphate | natural abundance | 20 mM |