Timeless couples G quadruplex detection with processing by DDX11 during DNA replication
DPSRRAPTWS PEEEAHLREL YLANKDVEGQ DVVEAILAHL NTVPRTRKQI IHHLVQMGLA DSVKDFQRKG THIVLWTGDQ ELELQRLFEE FRDSDDVLGH IMKNITAKRS RARIVDKLLA LGLVAERREL YKKRQKKLAS S
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 97.1 % (1645 of 1695) | 96.2 % (857 of 891) | 97.7 % (640 of 655) | 99.3 % (148 of 149) |
Backbone | 98.3 % (824 of 838) | 97.9 % (278 of 284) | 98.1 % (409 of 417) | 100.0 % (137 of 137) |
Sidechain | 96.3 % (955 of 992) | 95.4 % (579 of 607) | 97.9 % (365 of 373) | 91.7 % (11 of 12) |
Aromatic | 100.0 % (94 of 94) | 100.0 % (47 of 47) | 100.0 % (45 of 45) | 100.0 % (2 of 2) |
Methyl | 100.0 % (174 of 174) | 100.0 % (87 of 87) | 100.0 % (87 of 87) |
1. entity 1
DPSRRAPTWS PEEEAHLREL YLANKDVEGQ DVVEAILAHL NTVPRTRKQI IHHLVQMGLA DSVKDFQRKG THIVLWTGDQ ELELQRLFEE FRDSDDVLGH IMKNITAKRS RARIVDKLLA LGLVAERREL YKKRQKKLAS SSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4, Details 0.5 mM [U-13C; U-15N] TimDBD, 10 mM NA sodium phosphate, 40 mM NA potassium chloride, 0.5 mM NA EDTA, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TimDBD | [U-13C; U-15N] | 0.5 mM | |
2 | sodium phosphate | NA | 10 mM | |
3 | potassium chloride | NA | 40 mM | |
4 | EDTA | NA | 0.5 mM |