BMRBj - BMREntryMaster

Found 1 Document (0.484 seconds)

BMRB: 34459

6YCX

P. falciparum essential light chain, N-terminal domain

Authors

Weininger, U., Pazicky, S., Loew, C.

Assembly

Uncharacterized protein

Entity

1. Uncharacterized protein (polymer), 74 monomers, 8924.043 Da Detail

MASDMEEKFR EAFILFSSCS DHIEMYKFFE LMNSFGIILT NDEKAALPND INMDYWLNFA KKHYNYEQPF KHIN

Formula weight

8924.043 Da

Source organism

Plasmodium falciparum 3D7

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 91.1 %, Completeness (bb): 83.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.1 % (848 of 931)	97.9 % (475 of 485)	80.2 % (292 of 364)	98.8 % (81 of 82)
Backbone	83.2 % (366 of 440)	98.0 % (144 of 147)	68.3 % (151 of 221)	98.6 % (71 of 72)
Sidechain	98.2 % (554 of 564)	97.9 % (331 of 338)	98.6 % (213 of 216)	100.0 % (10 of 10)
Aromatic	100.0 % (136 of 136)	100.0 % (68 of 68)	100.0 % (67 of 67)	100.0 % (1 of 1)
Methyl	94.6 % (53 of 56)	92.9 % (26 of 28)	96.4 % (27 of 28)

1. entity 1

MASDMEEKFR EAFILFSSCS DHIEMYKFFE LMNSFGIILT NDEKAALPND INMDYWLNFA KKHYNYEQPF KHIN

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 288 (±0.5) K, pH 7.0 (±0.1), Details 1.0 mM [U-99% 13C; U-99% 15N] ELC 1-74, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	ELC 1-74	[U-99% 13C; U-99% 15N]	1.0 mM
2	Tris	natural abundance	50 mM
3	NaCl	natural abundance	20 mM
4	TCEP	natural abundance	0.5 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 288 (±0.5) K, pH 7.0 (±0.1), Details 1.0 mM 1-13C1 glucose ELC 1-74, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	ELC 1-74	1-13C1 glucose	1.0 mM
6	Tris	natural abundance	50 mM
7	NaCl	natural abundance	20 mM
8	TCEP	natural abundance	0.5 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 288 (±0.5) K, pH 7.0 (±0.1), Details 1.0 mM 2-13C1 glucose ELC 1-74, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
9	ELC 1-74	2-13C1 glucose	1.0 mM
10	Tris	natural abundance	50 mM
11	NaCl	natural abundance	20 mM
12	TCEP	natural abundance	0.5 mM

Release date

2019-11-26

Citation

Structural role of essential light chains in the apicomplexan glideosome
Pazicky, S., Dhamotharan, K., Kaszuba, K., Mertens, H., Gilberger, T., Svergun, D., Kosinski, J., Weininger, U., Loew, C.
Commun. Biol. (2020), 3, 568-568, PubMed 33051581 , DOI 10.1038/s42003-020-01283-8 , Abstract

Related entities 1. Uncharacterized protein, : 2 : 6 : 5 entities Detail

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 288 (±0.5) K, pH 7.0 (±0.1), Details 1.0 mM [U-99% 13C; U-99% 15N] ELC 1-74, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	ELC 1-74	[U-99% 13C; U-99% 15N]	1.0 mM
2	Tris	natural abundance	50 mM
3	NaCl	natural abundance	20 mM
4	TCEP	natural abundance	0.5 mM

2 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 288 (±0.5) K, pH 7.0 (±0.1), Details 1.0 mM [U-99% 13C; U-99% 15N] ELC 1-74, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	ELC 1-74	[U-99% 13C; U-99% 15N]	1.0 mM
2	Tris	natural abundance	50 mM
3	NaCl	natural abundance	20 mM
4	TCEP	natural abundance	0.5 mM

3 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 288 (±0.5) K, pH 7.0 (±0.1), Details 1.0 mM [U-99% 13C; U-99% 15N] ELC 1-74, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	ELC 1-74	[U-99% 13C; U-99% 15N]	1.0 mM
2	Tris	natural abundance	50 mM
3	NaCl	natural abundance	20 mM
4	TCEP	natural abundance	0.5 mM

4 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 288 (±0.5) K, pH 7.0 (±0.1), Details 1.0 mM 1-13C1 glucose ELC 1-74, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	ELC 1-74	1-13C1 glucose	1.0 mM
6	Tris	natural abundance	50 mM
7	NaCl	natural abundance	20 mM
8	TCEP	natural abundance	0.5 mM

5 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 288 (±0.5) K, pH 7.0 (±0.1), Details 1.0 mM 2-13C1 glucose ELC 1-74, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
9	ELC 1-74	2-13C1 glucose	1.0 mM
10	Tris	natural abundance	50 mM
11	NaCl	natural abundance	20 mM
12	TCEP	natural abundance	0.5 mM

6 3D HNCACB

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 288 (±0.5) K, pH 7.0 (±0.1), Details 1.0 mM [U-99% 13C; U-99% 15N] ELC 1-74, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	ELC 1-74	[U-99% 13C; U-99% 15N]	1.0 mM
2	Tris	natural abundance	50 mM
3	NaCl	natural abundance	20 mM
4	TCEP	natural abundance	0.5 mM

7 3D HN(COCA)CB

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 288 (±0.5) K, pH 7.0 (±0.1), Details 1.0 mM [U-99% 13C; U-99% 15N] ELC 1-74, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	ELC 1-74	[U-99% 13C; U-99% 15N]	1.0 mM
2	Tris	natural abundance	50 mM
3	NaCl	natural abundance	20 mM
4	TCEP	natural abundance	0.5 mM

8 3D H(CCO)NH

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 288 (±0.5) K, pH 7.0 (±0.1), Details 1.0 mM [U-99% 13C; U-99% 15N] ELC 1-74, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	ELC 1-74	[U-99% 13C; U-99% 15N]	1.0 mM
2	Tris	natural abundance	50 mM
3	NaCl	natural abundance	20 mM
4	TCEP	natural abundance	0.5 mM

9 3D H(CCO)NH

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 288 (±0.5) K, pH 7.0 (±0.1), Details 1.0 mM [U-99% 13C; U-99% 15N] ELC 1-74, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	ELC 1-74	[U-99% 13C; U-99% 15N]	1.0 mM
2	Tris	natural abundance	50 mM
3	NaCl	natural abundance	20 mM
4	TCEP	natural abundance	0.5 mM

10 3D HCCH-TOCSY

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 288 (±0.5) K, pH 7.0 (±0.1), Details 1.0 mM [U-99% 13C; U-99% 15N] ELC 1-74, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	ELC 1-74	[U-99% 13C; U-99% 15N]	1.0 mM
2	Tris	natural abundance	50 mM
3	NaCl	natural abundance	20 mM
4	TCEP	natural abundance	0.5 mM

11 2D (HB)CB(CGCD)HD

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 288 (±0.5) K, pH 7.0 (±0.1), Details 1.0 mM [U-99% 13C; U-99% 15N] ELC 1-74, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	ELC 1-74	[U-99% 13C; U-99% 15N]	1.0 mM
2	Tris	natural abundance	50 mM
3	NaCl	natural abundance	20 mM
4	TCEP	natural abundance	0.5 mM

12 2D HCCH-TOCSY aromatic

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 288 (±0.5) K, pH 7.0 (±0.1), Details 1.0 mM [U-99% 13C; U-99% 15N] ELC 1-74, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	ELC 1-74	[U-99% 13C; U-99% 15N]	1.0 mM
2	Tris	natural abundance	50 mM
3	NaCl	natural abundance	20 mM
4	TCEP	natural abundance	0.5 mM

13 3D 1H-15N NOESY

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 288 (±0.5) K, pH 7.0 (±0.1), Details 1.0 mM [U-99% 13C; U-99% 15N] ELC 1-74, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	ELC 1-74	[U-99% 13C; U-99% 15N]	1.0 mM
2	Tris	natural abundance	50 mM
3	NaCl	natural abundance	20 mM
4	TCEP	natural abundance	0.5 mM

14 3D 1H-13C NOESY aromatic

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 288 (±0.5) K, pH 7.0 (±0.1), Details 1.0 mM 1-13C1 glucose ELC 1-74, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	ELC 1-74	1-13C1 glucose	1.0 mM
6	Tris	natural abundance	50 mM
7	NaCl	natural abundance	20 mM
8	TCEP	natural abundance	0.5 mM

15 3D 1H-13C NOESY aliphatic

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 288 (±0.5) K, pH 7.0 (±0.1), Details 1.0 mM [U-99% 13C; U-99% 15N] ELC 1-74, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	ELC 1-74	[U-99% 13C; U-99% 15N]	1.0 mM
2	Tris	natural abundance	50 mM
3	NaCl	natural abundance	20 mM
4	TCEP	natural abundance	0.5 mM

16 3D 1H-13C NOESY aromatic

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 288 (±0.5) K, pH 7.0 (±0.1), Details 1.0 mM 2-13C1 glucose ELC 1-74, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
9	ELC 1-74	2-13C1 glucose	1.0 mM
10	Tris	natural abundance	50 mM
11	NaCl	natural abundance	20 mM
12	TCEP	natural abundance	0.5 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34459_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34459_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	937		100.0 (chain: 1, length: 74)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1

Status: Warning

Sequence coverage of experimental data

Entity_assembly_ID: 1, Chain length: 74, Sequence coverage: 100.0%

--------10--------20--------30--------40--------50--------60--------70----
MASDMEEKFREAFILFSSCSDHIEMYKFFELMNSFGIILTNDEKAALPNDINMDYWLNFAKKHYNYEQPFKHIN
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MASDMEEKFREAFILFSSCSDHIEMYKFFELMNSFGIILTNDEKAALPNDINMDYWLNFAKKHYNYEQPFKHIN

Total number of assignments
- ¹H chemical shifts: 566
- ¹³C chemical shifts: 290
- ¹⁵N chemical shifts: 81

Completeness of assignments

Entity_assemble_ID: 1

Excluded Atom_ID in statistics

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
8	LYS	HZ1	7.351
8	LYS	HZ2	7.351
8	LYS	HZ3	7.351
27	LYS	HZ1	7.186
27	LYS	HZ2	7.186
27	LYS	HZ3	7.186
44	LYS	HZ1	7.252
44	LYS	HZ2	7.252
44	LYS	HZ3	7.252
61	LYS	HZ1	7.262
61	LYS	HZ2	7.262
61	LYS	HZ3	7.262
62	LYS	HZ1	7.343
62	LYS	HZ2	7.343
62	LYS	HZ3	7.343
71	LYS	HZ1	7.262
71	LYS	HZ2	7.262
71	LYS	HZ3	7.262

All atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	485	482	99.4
¹³C chemical shifts	364	290	79.7
¹⁵N chemical shifts	82	81	98.8

Backbone atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	147	146	99.3
¹³C chemical shifts	148	74	50.0
¹⁵N chemical shifts	72	71	98.6

Side chain atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	338	336	99.4
¹³C chemical shifts	216	216	100.0
¹⁵N chemical shifts	10	10	100.0

Methyl group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	33	33	100.0
¹³C chemical shifts	33	33	100.0

Aromatic group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	68	68	100.0
¹³C chemical shifts	67	67	100.0
¹⁵N chemical shifts	1	1	100.0

Redox state of CYS
Peptide bond of PRO
Tautomeric state of HIS
Rotameric state of ILE , LEU, and VAL
Histogram of normalized assigned chemical shifts
Random coil index and derived parameters (S² and NMR RMSD)
- Chain_ID: 1

Keywords MOTOR PROTEIN, glideosome, light chain, motility, myosin

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Found 1 Document (0.484 seconds)

BMRB: 34459

Sample #1

Sample #2

Sample #3

Related entities 1. Uncharacterized protein, : 2 : 6 : 5 entities Detail

Experiments performed 16 experiments Detail

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Chemical shift validation 3 contents Detail