Structural and DNA Binding Properties of Mycobacterial Integration Host Factor mIHF
GSHMVALPQL TDEQRAAALE KAAAARRARA ELKDRLKRGG TNLTQVLKDA ESDEVLGKMK VSALLEALPK VGKVKAQEIM TELEIAPTRR LRGLGDRQRK ALLEKFGSA
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 77.7 % (971 of 1250) | 81.3 % (536 of 659) | 69.3 % (332 of 479) | 92.0 % (103 of 112) |
Backbone | 77.9 % (505 of 648) | 90.1 % (201 of 223) | 64.6 % (206 of 319) | 92.5 % (98 of 106) |
Sidechain | 79.9 % (562 of 703) | 76.8 % (335 of 436) | 85.1 % (222 of 261) | 83.3 % (5 of 6) |
Aromatic | 0.0 % (0 of 14) | 0.0 % (0 of 7) | 0.0 % (0 of 7) | |
Methyl | 90.0 % (126 of 140) | 90.0 % (63 of 70) | 90.0 % (63 of 70) |
1. entity 1
GSHMVALPQL TDEQRAAALE KAAAARRARA ELKDRLKRGG TNLTQVLKDA ESDEVLGKMK VSALLEALPK VGKVKAQEIM TELEIAPTRR LRGLGDRQRK ALLEKFGSASolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 1.34 mM [U-13C; U-15N] Mycobacterial integration host factor (mIHF), 50 mM sodium phosphate, 100 mM sodium chloride, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Mycobacterial integration host factor (mIHF) | [U-13C; U-15N] | 1.34 mM | |
2 | sodium phosphate | natural abundance | 50 mM | |
3 | sodium chloride | natural abundance | 100 mM |