Structure of a protein-RNA complex by ssNMR
MAKPSYVKFE VPKELAEKAL QAVEIARDTG KIRKGTNETT KAVERGQAKL VIIAEDVDPE EIVAHLPPLC EEKEIPYIYV PSKKELGAAA GIEVAAASVA IIEPGKARDL VEEIAMKVKE LMK
Polymer type: polypeptide(L) polyribonucleotide
Total | 13C | 15N | |
---|---|---|---|
All | 50.4 % (437 of 867) | 46.6 % (343 of 736) | 71.8 % (94 of 131) |
Backbone | 59.9 % (364 of 608) | 54.8 % (270 of 493) | 81.7 % (94 of 115) |
Sidechain | 39.6 % (149 of 376) | 41.4 % (149 of 360) | 0.0 % (0 of 16) |
Aromatic | 0.0 % (0 of 76) | 0.0 % (0 of 63) | 0.0 % (0 of 13) |
Methyl | 76.2 % (64 of 84) | 76.2 % (64 of 84) |
1. entity 1
MAKPSYVKFE VPKELAEKAL QAVEIARDTG KIRKGTNETT KAVERGQAKL VIIAEDVDPE EIVAHLPPLC EEKEIPYIYV PSKKELGAAA GIEVAAASVA IIEPGKARDL VEEIAMKVKE LMK2. entity 2
GCUGAGCUCG AAAGAGCAAU GAUGUCSolvent system solid, Pressure 1 bar, Temperature 265 K, pH 7.6, Details 4 mg/mL [U-99% 13C; U-99% 15N] 50S RIBOSOMAL PROTEIN L7AE, solid
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | 50S RIBOSOMAL PROTEIN L7AE (U-13C, U-15N) | [U-99% 13C; U-99% 15N] | 4 mg/mL | |
2 | HEPES | natural abundance | 25 mM | |
3 | NaCl | natural abundance | 120 mM |
Solvent system solid, Pressure 1 bar, Temperature 265 K, pH 7.6, Details 8 mg/mL [U-99% 13C; U-99% 15N] 50S RIBOSOMAL PROTEIN L7AE, 5 mg/mL RNA (26-MER), solid
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | 50S RIBOSOMAL PROTEIN L7AE (U-13C, U-15N) | [U-99% 13C; U-99% 15N] | 8 mg/mL | |
5 | RNA (26-MER) | natural abundance | 5 mg/mL | |
6 | HEPES | natural abundance | 25 mM | |
7 | NaCl | natural abundance | 120 mM |
Solvent system solid, Pressure 1 bar, Temperature 265 K, pH 7.6, Details 4 mg/mL [U-99% 13C; U-99% 15N] 50S RIBOSOMAL PROTEIN L7AE, 12 mg/mL 50S RIBOSOMAL PROTEIN L7AE, 10 mg/mL RNA (26-MER), solid
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | 50S RIBOSOMAL PROTEIN L7AE (U-13C, U-15N) | [U-99% 13C; U-99% 15N] | 4 mg/mL | |
9 | RNA (26-MER) | natural abundance | 10 mg/mL | |
10 | 50S RIBOSOMAL PROTEIN L7AE | natural abundance | 12 mg/mL | |
11 | HEPES | natural abundance | 25 mM | |
12 | NaCl | natural abundance | 120 mM |
Solvent system solid, Pressure 1 bar, Temperature 265 K, pH 7.6, Details 16 mg/mL 50S RIBOSOMAL PROTEIN L7AE, 2 mg/mL [U-13C; U-15N]-Ade RNA (26-MER), 8 mg/mL RNA (26-MER), solid
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | RNA (26-MER) | natural abundance | 8 mg/mL | |
14 | 50S RIBOSOMAL PROTEIN L7AE | natural abundance | 16 mg/mL | |
15 | RNA (26-MER) (U-13C, U-15N-ade) | [U-13C; U-15N]-Ade | 2 mg/mL | |
16 | HEPES | natural abundance | 25 mM | |
17 | NaCl | natural abundance | 120 mM |
Solvent system solid, Pressure 1 bar, Temperature 265 K, pH 7.6, Details 16 mg/mL 50S RIBOSOMAL PROTEIN L7AE, 2 mg/mL [U-13C; U-15N]-Ura RNA (26-MER), 8 mg/mL RNA (26-MER), solid
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
18 | RNA (26-MER) | natural abundance | 8 mg/mL | |
19 | 50S RIBOSOMAL PROTEIN L7AE | natural abundance | 16 mg/mL | |
20 | RNA (26-MER) (U-13C, U-15N-ura) | [U-13C; U-15N]-Ura | 2 mg/mL | |
21 | HEPES | natural abundance | 25 mM | |
22 | NaCl | natural abundance | 120 mM |
Solvent system solid, Pressure 1 bar, Temperature 265 K, pH 7.6, Details 4 mg/mL [U-99% 13C; U-99% 15N] 50S RIBOSOMAL PROTEIN L7AE, 12 mg/mL 50S RIBOSOMAL PROTEIN L7AE, 10 mg/mL RNA (26-MER), solid
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
23 | 50S RIBOSOMAL PROTEIN L7AE (U-13C, U-15N) | [U-99% 13C; U-99% 15N] | 4 mg/mL | |
24 | RNA (26-MER) | natural abundance | 10 mg/mL | |
25 | 50S RIBOSOMAL PROTEIN L7AE | natural abundance | 12 mg/mL | |
26 | HEPES | natural abundance | 25 mM | |
27 | NaCl | natural abundance | 120 mM |
Solvent system solid, Pressure 1 bar, Temperature 265 K, pH 7.6, Details 16 mg/mL 50S RIBOSOMAL PROTEIN L7AE, 2 mg/mL [U-13C; U-15N]-Ade RNA (26-MER), 8 mg/mL RNA (26-MER), solid
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
28 | RNA (26-MER) | natural abundance | 8 mg/mL | |
29 | 50S RIBOSOMAL PROTEIN L7AE | natural abundance | 16 mg/mL | |
30 | RNA (26-MER) (U-13C, U-15N-ade) | [U-13C; U-15N]-Ade | 2 mg/mL | |
31 | HEPES | natural abundance | 25 mM | |
32 | NaCl | natural abundance | 120 mM |
Solvent system solid, Pressure 1 bar, Temperature 265 K, pH 7.6, Details 16 mg/mL 50S RIBOSOMAL PROTEIN L7AE, 2 mg/mL [U-13C; U-15N]-Ura RNA (26-MER), 8 mg/mL RNA (26-MER), solid
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
33 | RNA (26-MER) | natural abundance | 8 mg/mL | |
34 | 50S RIBOSOMAL PROTEIN L7AE | natural abundance | 16 mg/mL | |
35 | RNA (26-MER) (U-13C, U-15N-ura) | [U-13C; U-15N]-Ura | 2 mg/mL | |
36 | HEPES | natural abundance | 25 mM | |
37 | NaCl | natural abundance | 120 mM |
Solvent system solid, Pressure 1 bar, Temperature 265 K, pH 7.6, Details 4 mg/mL [U-99% 13C; U-99% 15N] 50S RIBOSOMAL PROTEIN L7AE, 12 mg/mL 50S RIBOSOMAL PROTEIN L7AE, 10 mg/mL RNA (26-MER), solid
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
38 | 50S RIBOSOMAL PROTEIN L7AE (U-13C, U-15N) | [U-99% 13C; U-99% 15N] | 4 mg/mL | |
39 | RNA (26-MER) | natural abundance | 10 mg/mL | |
40 | 50S RIBOSOMAL PROTEIN L7AE | natural abundance | 12 mg/mL | |
41 | HEPES | natural abundance | 25 mM | |
42 | NaCl | natural abundance | 120 mM |
Solvent system solid, Pressure 1 bar, Temperature 265 K, pH 7.6, Details 16 mg/mL 50S RIBOSOMAL PROTEIN L7AE, 2 mg/mL [U-13C; U-15N]-Ade RNA (26-MER), 8 mg/mL RNA (26-MER), solid
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
43 | RNA (26-MER) | natural abundance | 8 mg/mL | |
44 | 50S RIBOSOMAL PROTEIN L7AE | natural abundance | 16 mg/mL | |
45 | RNA (26-MER) (U-13C, U-15N-ade) | [U-13C; U-15N]-Ade | 2 mg/mL | |
46 | HEPES | natural abundance | 25 mM | |
47 | NaCl | natural abundance | 120 mM |
Solvent system solid, Pressure 1 bar, Temperature 265 K, pH 7.6, Details 16 mg/mL 50S RIBOSOMAL PROTEIN L7AE, 2 mg/mL [U-13C; U-15N]-Ura RNA (26-MER), 8 mg/L RNA (26-MER), solid
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
48 | RNA (26-MER) | natural abundance | 8 mg/mL | |
49 | 50S RIBOSOMAL PROTEIN L7AE | natural abundance | 16 mg/mL | |
50 | RNA (26-MER) (U-13C, U-15N-ura) | [U-13C; U-15N]-Ura | 2 mg/mL | |
51 | HEPES | natural abundance | 25 mM | |
52 | NaCl | natural abundance | 120 mM |