BMRB: 34484

Constitutive decay element CDE2 from human 3'UTR

Authors

Schwalbe, H., Binas, O.

Assembly

RNA (5'-R(*GP*GP*UP*GP*CP*CP*UP*AP*AP*UP*AP*UP*UP*UP*AP*GP*GP*CP*AP*CP*C)-3')

Entity

1. RNA (5'-R(*GP*GP*UP*GP*CP*CP*UP*AP*AP*UP*AP*UP*UP*UP*AP*GP*GP*CP*AP*CP*C)-3') (polymer, Thiol state: not present), 21 monomers, 6752.975 Da Detail

GGUGCCUAAU AUUUAGGCAC C

Formula weight

6752.975 Da

Source organism

Thermosinus carboxydivorans Nor1

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chemcal Shifts

¹H: 159, ¹³C: 94 assignments Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val	Val err	Ambig. code
1	1	1	G	H1'	H	5.843	0.02
2	1	1	G	H2'	H	4.961	0.02
3	1	1	G	H3'	H	4.695	0.02
4	1	1	G	H4'	H	4.572	0.02
5	1	1	G	H5'	H	4.458	0.02
6	1	1	G	H5''	H	4.284	0.02
7	1	1	G	H8	H	8.154	0.02
8	1	1	G	C1'	C	91.893	0.02
9	1	1	G	C2'	C	75.074	0.02
10	1	1	G	C3'	C	74.591	0.02
11	1	1	G	C4'	C	83.275	0.02
12	1	1	G	C5'	C	67.161	0.02
13	1	2	G	H1'	H	5.936	0.02
14	1	2	G	H2'	H	4.526	0.02
15	1	2	G	H3'	H	4.559	0.02
16	1	2	G	H4'	H	4.493	0.02
17	1	2	G	H5'	H	4.411	0.02
18	1	2	G	H5''	H	4.242	0.02
19	1	2	G	H8	H	7.541	0.02
20	1	2	G	C1'	C	93.094	0.02
21	1	2	G	C3'	C	73.108	0.02
22	1	2	G	C5'	C	66.829	0.02
23	1	3	U	H1'	H	5.591	0.02
24	1	3	U	H2'	H	4.631	0.02
25	1	3	U	H3'	H	4.594	0.02
26	1	3	U	H4'	H	4.475	0.02
27	1	3	U	H5	H	5.141	0.02
28	1	3	U	H5'	H	4.605	0.02
29	1	3	U	H5''	H	4.140	0.02
30	1	3	U	H6	H	7.784	0.02
31	1	3	U	C1'	C	93.552	0.02
32	1	3	U	C2'	C	75.378	0.02
33	1	3	U	C3'	C	72.352	0.02
34	1	3	U	C4'	C	82.065	0.02
35	1	3	U	C5'	C	64.503	0.02
36	1	4	G	H1'	H	5.825	0.02
37	1	4	G	H2'	H	4.527	0.02
38	1	4	G	H3'	H	4.590	0.02
39	1	4	G	H4'	H	4.511	0.02
40	1	4	G	H5'	H	4.530	0.02
41	1	4	G	H5''	H	4.160	0.02
42	1	4	G	H8	H	7.739	0.02
43	1	4	G	C1'	C	92.618	0.02
44	1	4	G	C2'	C	75.367	0.02
45	1	4	G	C3'	C	73.161	0.02
46	1	4	G	C4'	C	82.025	0.02
47	1	4	G	C5'	C	65.721	0.02
48	1	5	C	H1'	H	5.459	0.02
49	1	5	C	H2'	H	4.401	0.02
50	1	5	C	H4'	H	4.523	0.02
51	1	5	C	H5	H	5.270	0.02
52	1	5	C	H5''	H	4.085	0.02
53	1	5	C	H6	H	7.656	0.02
54	1	5	C	C1'	C	93.895	0.02
55	1	6	C	H1'	H	5.460	0.02
56	1	6	C	H2'	H	4.352	0.02
57	1	6	C	H5	H	5.514	0.02
58	1	6	C	H5'	H	4.436	0.02
59	1	6	C	H5''	H	4.065	0.02
60	1	6	C	H6	H	7.735	0.02
61	1	6	C	C1'	C	94.362	0.02
62	1	6	C	C2'	C	75.351	0.02
63	1	7	U	H1'	H	5.455	0.02
64	1	7	U	H2'	H	4.402	0.02
65	1	7	U	H3'	H	4.610	0.02
66	1	7	U	H4'	H	4.462	0.02
67	1	7	U	H5	H	5.419	0.02
68	1	7	U	H5'	H	4.560	0.02
69	1	7	U	H5''	H	4.094	0.02
70	1	7	U	H6	H	7.848	0.02
71	1	7	U	C1'	C	93.728	0.02
72	1	7	U	C2'	C	75.449	0.02
73	1	7	U	C3'	C	72.282	0.02
74	1	8	A	H1'	H	5.865	0.02
75	1	8	A	H2	H	6.651	0.02
76	1	8	A	H2'	H	4.420	0.02
77	1	8	A	H3'	H	4.681	0.02
78	1	8	A	H4'	H	4.467	0.02
79	1	8	A	H5'	H	4.522	0.02
80	1	8	A	H5''	H	4.155	0.02
81	1	8	A	H8	H	8.032	0.02
82	1	8	A	C1'	C	92.710	0.02
83	1	8	A	C2'	C	75.812	0.02
84	1	8	A	C3'	C	73.087	0.02
85	1	8	A	C4'	C	82.330	0.02
86	1	8	A	C5'	C	65.330	0.02
87	1	9	A	H1'	H	5.702	0.02
88	1	9	A	H2	H	7.728	0.02
89	1	9	A	H2'	H	4.399	0.02
90	1	9	A	H3'	H	4.563	0.02
91	1	9	A	H4'	H	4.415	0.02
92	1	9	A	H5'	H	4.443	0.02
93	1	9	A	H5''	H	4.081	0.02
94	1	9	A	H8	H	7.683	0.02
95	1	9	A	C1'	C	91.713	0.02
96	1	9	A	C2'	C	76.290	0.02
97	1	9	A	C3'	C	74.431	0.02
98	1	9	A	C4'	C	83.189	0.02
99	1	9	A	C5'	C	65.974	0.02
100	1	10	U	H1'	H	5.528	0.02
101	1	10	U	H2'	H	4.206	0.02
102	1	10	U	H3'	H	4.400	0.02
103	1	10	U	H4'	H	4.214	0.02
104	1	10	U	H5	H	5.528	0.02
105	1	10	U	H5'	H	4.085	0.02
106	1	10	U	H5''	H	3.943	0.02
107	1	10	U	H6	H	7.543	0.02
108	1	10	U	C1'	C	90.902	0.02
109	1	10	U	C2'	C	75.571	0.02
110	1	10	U	C3'	C	76.450	0.02
111	1	10	U	C4'	C	84.747	0.02
112	1	10	U	C5'	C	66.980	0.02
113	1	11	A	H1'	H	5.799	0.02
114	1	11	A	H2	H	8.003	0.02
115	1	11	A	H2'	H	4.561	0.02
116	1	11	A	H3'	H	4.638	0.02
117	1	11	A	H4'	H	4.413	0.02
118	1	11	A	H5'	H	4.114	0.02
119	1	11	A	H5''	H	4.000	0.02
120	1	11	A	H8	H	8.011	0.02
121	1	11	A	C1'	C	90.218	0.02
122	1	11	A	C2'	C	77.184	0.02
123	1	11	A	C3'	C	77.921	0.02
124	1	11	A	C4'	C	86.000	0.02
125	1	11	A	C5'	C	67.831	0.02
126	1	12	U	H1'	H	5.920	0.02
127	1	12	U	H2'	H	4.380	0.02
128	1	12	U	H3'	H	4.684	0.02
129	1	12	U	H4'	H	4.454	0.02
130	1	12	U	H5	H	5.772	0.02
131	1	12	U	H5'	H	4.153	0.02
132	1	12	U	H5''	H	4.089	0.02
133	1	12	U	H6	H	7.744	0.02
134	1	12	U	C1'	C	90.467	0.02
135	1	12	U	C2'	C	75.606	0.02
136	1	12	U	C3'	C	77.003	0.02
137	1	12	U	C4'	C	85.166	0.02
138	1	12	U	C5'	C	67.992	0.02
139	1	13	U	H1'	H	5.858	0.02
140	1	13	U	H2'	H	4.557	0.02
141	1	13	U	H3'	H	4.596	0.02
142	1	13	U	H4'	H	4.516	0.02
143	1	13	U	H5	H	5.902	0.02
144	1	13	U	H5'	H	4.247	0.02
145	1	13	U	H5''	H	4.201	0.02
146	1	13	U	H6	H	7.903	0.02
147	1	13	U	C1'	C	91.160	0.02
148	1	13	U	C2'	C	74.889	0.02
149	1	13	U	C3'	C	76.425	0.02
150	1	13	U	C4'	C	84.607	0.02
151	1	13	U	C5'	C	68.415	0.02
152	1	14	U	H1'	H	5.698	0.02
153	1	14	U	H2'	H	4.592	0.02
154	1	14	U	H3'	H	4.553	0.02
155	1	14	U	H4'	H	4.556	0.02
156	1	14	U	H5	H	5.802	0.02
157	1	14	U	H5'	H	4.418	0.02
158	1	14	U	H5''	H	4.308	0.02
159	1	14	U	H6	H	7.949	0.02
160	1	14	U	C1'	C	93.559	0.02
161	1	14	U	C2'	C	75.149	0.02
162	1	14	U	C3'	C	74.054	0.02
163	1	14	U	C4'	C	83.097	0.02
164	1	14	U	C5'	C	67.182	0.02
165	1	15	A	H1'	H	5.999	0.02
166	1	15	A	H2	H	6.746	0.02
167	1	15	A	H2'	H	4.649	0.02
168	1	15	A	H3'	H	4.732	0.02
169	1	15	A	H4'	H	4.540	0.02
170	1	15	A	H5'	H	4.562	0.02
171	1	15	A	H5''	H	4.246	0.02
172	1	15	A	H8	H	8.208	0.02
173	1	15	A	C1'	C	92.478	0.02
174	1	15	A	C2'	C	75.731	0.02
175	1	15	A	C3'	C	73.135	0.02
176	1	15	A	C4'	C	82.138	0.02
177	1	15	A	C5'	C	65.690	0.02
178	1	16	G	H1'	H	5.534	0.02
179	1	16	G	H2'	H	4.476	0.02
180	1	16	G	H3'	H	4.422	0.02
181	1	16	G	H4'	H	4.436	0.02
182	1	16	G	H5'	H	4.447	0.02
183	1	16	G	H5''	H	4.081	0.02
184	1	16	G	H8	H	7.156	0.02
185	1	16	G	C1'	C	92.402	0.02
186	1	16	G	C2'	C	75.468	0.02
187	1	16	G	C3'	C	73.025	0.02
188	1	16	G	C4'	C	81.940	0.02
189	1	16	G	C5'	C	65.868	0.02
190	1	17	G	H1'	H	5.662	0.02
191	1	17	G	H2'	H	4.423	0.02
192	1	17	G	H3'	H	4.450	0.02
193	1	17	G	H4'	H	4.433	0.02
194	1	17	G	H5'	H	4.463	0.02
195	1	17	G	H5''	H	4.033	0.02
196	1	17	G	H8	H	7.154	0.02
197	1	17	G	C1'	C	92.934	0.02
198	1	17	G	C2'	C	75.319	0.02
199	1	17	G	C3'	C	72.759	0.02
200	1	17	G	C4'	C	81.964	0.02
201	1	17	G	C5'	C	65.270	0.02
202	1	18	C	H1'	H	5.484	0.02
203	1	18	C	H2'	H	4.527	0.02
204	1	18	C	H3'	H	4.515	0.02
205	1	18	C	H4'	H	4.411	0.02
206	1	18	C	H5	H	5.212	0.02
207	1	18	C	H5'	H	4.549	0.02
208	1	18	C	H5''	H	4.067	0.02
209	1	18	C	H6	H	7.618	0.02
210	1	18	C	C1'	C	93.749	0.02
211	1	18	C	C2'	C	75.473	0.02
212	1	18	C	C3'	C	72.273	0.02
213	1	18	C	C4'	C	81.926	0.02
214	1	18	C	C5'	C	64.753	0.02
215	1	19	A	H1'	H	5.940	0.02
216	1	19	A	H2	H	7.429	0.02
217	1	19	A	H2'	H	4.575	0.02
218	1	19	A	H3'	H	4.630	0.02
219	1	19	A	H4'	H	4.566	0.02
220	1	19	A	H5'	H	4.528	0.02
221	1	19	A	H5''	H	4.133	0.02
222	1	19	A	H8	H	8.038	0.02
223	1	19	A	C1'	C	93.195	0.02
224	1	19	A	C2'	C	75.559	0.02
225	1	19	A	C3'	C	72.926	0.02
226	1	19	A	C4'	C	82.521	0.02
227	1	19	A	C5'	C	65.233	0.02
228	1	20	C	H1'	H	5.406	0.02
229	1	20	C	H2'	H	4.068	0.02
230	1	20	C	H3'	H	4.356	0.02
231	1	20	C	H4'	H	4.360	0.02
232	1	20	C	H5	H	5.218	0.02
233	1	20	C	H5'	H	4.507	0.02
234	1	20	C	H5''	H	4.022	0.02
235	1	20	C	H6	H	7.514	0.02
236	1	20	C	C1'	C	94.224	0.02
237	1	20	C	C2'	C	75.767	0.02
238	1	20	C	C3'	C	72.302	0.02
239	1	20	C	C4'	C	82.036	0.02
240	1	20	C	C5'	C	64.587	0.02
241	1	21	C	H1'	H	6.099	0.02
242	1	21	C	H2'	H	4.626	0.02
243	1	21	C	H3'	H	4.935	0.02
244	1	21	C	H4'	H	4.399	0.02
245	1	21	C	H5	H	5.472	0.02
246	1	21	C	H5'	H	4.503	0.02
247	1	21	C	H5''	H	4.075	0.02
248	1	21	C	H6	H	7.457	0.02
249	1	21	C	C1'	C	91.985	0.02
250	1	21	C	C2'	C	85.173	0.02
251	1	21	C	C3'	C	77.977	0.02
252	1	21	C	C4'	C	85.572	0.02
253	1	21	C	C5'	C	65.759	0.02

Release date

2020-05-21

Citation

Structural basis for the recognition of transiently structured AU-rich elements by Roquin
Binas, O., Tants, J.N., Peter, S., Janowski, R., Davydova, E., Braun, J., Niessing, D., Schwalbe, H., Weigand, J.E., Schlundt, A.
Nucleic Acids Res. (2020), 48, 7385-7403, PubMed 32491174 , DOI 10.1093/nar/gkaa465 , Abstract

Related entities 1. RNA (5'-R(*GP*GP*UP*GP*CP*CP*UP*AP*AP*UP*AP*UP*UP*UP*AP*GP*GP*CP*AP*CP*C)-3'), : 1 : 1 : 1 entities Detail

Experiments performed 9 experiments Detail

Chemical shift validation 3 contents Detail