BMRBj - BMREntryMaster

Found 1 Document (0.588 seconds)

BMRB: 34513

6YP5

Solution NMR structure of the oligomerization domain of respiratory syncytial virus phosphoprotein

Authors

Cardone, C., Bontems, F., Bardiaux, B., Sizun, C.

Assembly

Phosphoprotein

Entity

1. Phosphoprotein (polymer, Thiol state: not present), 45 monomers, 4555.993 × 4 Da Detail

GSGSGSGSTN DNITARLDRI DEKLSEILGM LHTLVVASAG PTSAR

Total weight

18223.973 Da

Max. entity weight

4555.993 Da

Source organism

Respiratory syncytial virus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.6 %, Completeness (bb): 90.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.6 % (426 of 460)	94.8 % (221 of 233)	93.3 % (166 of 178)	79.6 % (39 of 49)
Backbone	90.7 % (243 of 268)	92.6 % (88 of 95)	91.5 % (118 of 129)	84.1 % (37 of 44)
Sidechain	96.5 % (223 of 231)	97.1 % (134 of 138)	98.9 % (87 of 88)	40.0 % (2 of 5)
Aromatic	50.0 % (2 of 4)	50.0 % (1 of 2)	50.0 % (1 of 2)
Methyl	100.0 % (56 of 56)	100.0 % (28 of 28)	100.0 % (28 of 28)

1. entity 1

GSGSGSGSTN DNITARLDRI DEKLSEILGM LHTLVVASAG PTSAR

Show sample condition

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 600 uM [U-13C; U-15N] Phosphoprotein(127-163), 20 mM sodium phosphate, 100 mM sodium chloride, 93% H2O/7% D2O

#	Name	Isotope labeling	Concentration
1	Phosphoprotein(127-163) [U-13C; U-15N]	[U-13C; U-15N]	600 (±50.0) uM
2	sodium phosphate	natural abundance	20 (±2.0) mM
3	sodium chloride	natural abundance	100 (±10.0) mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 600 uM [U-13C; U-15N] Phosphoprotein(127-163), 20 mM sodium phosphate, 100 mM sodium chloride, 100% D2O

#	Name	Isotope labeling	Concentration
4	Phosphoprotein(127-163) [U-13C; U-15N]	[U-13C; U-15N]	600 (±50.0) uM
5	sodium phosphate	natural abundance	20 (±2.0) mM
6	sodium chloride	natural abundance	100 (±10.0) mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 300 uM [U-13C; U-15N] Phosphoprotein(127-163), 300 uM Phosphoprotein(127-163), 20 mM sodium phosphate, 100 mM sodium chloride, 100% D2O

#	Name	Isotope labeling	Concentration
7	Phosphoprotein(127-163) [U-13C; U-15N]	[U-13C; U-15N]	300 (±30.0) uM
8	sodium phosphate	natural abundance	20 (±2.0) mM
9	sodium chloride	natural abundance	100 (±10.0) mM
10	Phosphoprotein(127-163)	natural abundance	300 (±30.0) uM

Release date

2020-04-16

Citation

A Structural and Dynamic Analysis of the Partially Disordered Polymerase-Binding Domain in RSV Phosphoprotein
Cardone, C., Caseau, C.M., Bardiaux, B., Thureaux, A., Galloux, M., Bajorek, M., Eleouet, J.F., Litaudon, M., Bontems, F., Sizun, C.
Biomolecules (2021), 11, 1225-1225, PubMed 34439894 , DOI 10.3390/biom11081225 , Abstract

Related entities 1. Phosphoprotein, : 1 : 1 : 14 entities Detail

Interaction partners 1. Phosphoprotein, : 4 interactors Detail

More details for 4 interactors identified by 3 citations

Experiments performed 19 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 600 uM [U-13C; U-15N] Phosphoprotein(127-163), 20 mM sodium phosphate, 100 mM sodium chloride, 93% H2O/7% D2O

#	Name	Isotope labeling	Concentration
1	Phosphoprotein(127-163) [U-13C; U-15N]	[U-13C; U-15N]	600 (±50.0) uM
2	sodium phosphate	natural abundance	20 (±2.0) mM
3	sodium chloride	natural abundance	100 (±10.0) mM

2 3D HNCA

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Phosphoprotein(127-163) [U-13C; U-15N]	[U-13C; U-15N]	600 (±50.0) uM
2	sodium phosphate	natural abundance	20 (±2.0) mM
3	sodium chloride	natural abundance	100 (±10.0) mM

3 3D HN(CO)CA

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Phosphoprotein(127-163) [U-13C; U-15N]	[U-13C; U-15N]	600 (±50.0) uM
2	sodium phosphate	natural abundance	20 (±2.0) mM
3	sodium chloride	natural abundance	100 (±10.0) mM

4 3D HNCACB

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Phosphoprotein(127-163) [U-13C; U-15N]	[U-13C; U-15N]	600 (±50.0) uM
2	sodium phosphate	natural abundance	20 (±2.0) mM
3	sodium chloride	natural abundance	100 (±10.0) mM

5 3D HNCO

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Phosphoprotein(127-163) [U-13C; U-15N]	[U-13C; U-15N]	600 (±50.0) uM
2	sodium phosphate	natural abundance	20 (±2.0) mM
3	sodium chloride	natural abundance	100 (±10.0) mM

6 2D 1H-13C HSQC

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Phosphoprotein(127-163) [U-13C; U-15N]	[U-13C; U-15N]	600 (±50.0) uM
2	sodium phosphate	natural abundance	20 (±2.0) mM
3	sodium chloride	natural abundance	100 (±10.0) mM

7 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Phosphoprotein(127-163) [U-13C; U-15N]	[U-13C; U-15N]	600 (±50.0) uM
2	sodium phosphate	natural abundance	20 (±2.0) mM
3	sodium chloride	natural abundance	100 (±10.0) mM

8 2D 1H-13C HSQC

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 600 uM [U-13C; U-15N] Phosphoprotein(127-163), 20 mM sodium phosphate, 100 mM sodium chloride, 100% D2O

#	Name	Isotope labeling	Concentration
4	Phosphoprotein(127-163) [U-13C; U-15N]	[U-13C; U-15N]	600 (±50.0) uM
5	sodium phosphate	natural abundance	20 (±2.0) mM
6	sodium chloride	natural abundance	100 (±10.0) mM

9 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
4	Phosphoprotein(127-163) [U-13C; U-15N]	[U-13C; U-15N]	600 (±50.0) uM
5	sodium phosphate	natural abundance	20 (±2.0) mM
6	sodium chloride	natural abundance	100 (±10.0) mM

10 3D HCCH-TOCSY

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
4	Phosphoprotein(127-163) [U-13C; U-15N]	[U-13C; U-15N]	600 (±50.0) uM
5	sodium phosphate	natural abundance	20 (±2.0) mM
6	sodium chloride	natural abundance	100 (±10.0) mM

11 3D CBCA(CO)NH

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Phosphoprotein(127-163) [U-13C; U-15N]	[U-13C; U-15N]	600 (±50.0) uM
2	sodium phosphate	natural abundance	20 (±2.0) mM
3	sodium chloride	natural abundance	100 (±10.0) mM

12 3D HBHA(CO)NH

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Phosphoprotein(127-163) [U-13C; U-15N]	[U-13C; U-15N]	600 (±50.0) uM
2	sodium phosphate	natural abundance	20 (±2.0) mM
3	sodium chloride	natural abundance	100 (±10.0) mM

13 3D HN(COCA)CB

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Phosphoprotein(127-163) [U-13C; U-15N]	[U-13C; U-15N]	600 (±50.0) uM
2	sodium phosphate	natural abundance	20 (±2.0) mM
3	sodium chloride	natural abundance	100 (±10.0) mM

14 3D 1H-13C NOESY

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
4	Phosphoprotein(127-163) [U-13C; U-15N]	[U-13C; U-15N]	600 (±50.0) uM
5	sodium phosphate	natural abundance	20 (±2.0) mM
6	sodium chloride	natural abundance	100 (±10.0) mM

15 2D 1H-13C HSQC

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
4	Phosphoprotein(127-163) [U-13C; U-15N]	[U-13C; U-15N]	600 (±50.0) uM
5	sodium phosphate	natural abundance	20 (±2.0) mM
6	sodium chloride	natural abundance	100 (±10.0) mM

16 1D 1H

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
4	Phosphoprotein(127-163) [U-13C; U-15N]	[U-13C; U-15N]	600 (±50.0) uM
5	sodium phosphate	natural abundance	20 (±2.0) mM
6	sodium chloride	natural abundance	100 (±10.0) mM

17 3D 1H-15N NOESY-HSQC

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Phosphoprotein(127-163) [U-13C; U-15N]	[U-13C; U-15N]	600 (±50.0) uM
2	sodium phosphate	natural abundance	20 (±2.0) mM
3	sodium chloride	natural abundance	100 (±10.0) mM

18 1H-13C NOESY-HSQC with 13C/15N filter in F1

Instrument

Bruker AVANCE III - 950 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 300 uM [U-13C; U-15N] Phosphoprotein(127-163), 300 uM Phosphoprotein(127-163), 20 mM sodium phosphate, 100 mM sodium chloride, 100% D2O

#	Name	Isotope labeling	Concentration
7	Phosphoprotein(127-163) [U-13C; U-15N]	[U-13C; U-15N]	300 (±30.0) uM
8	sodium phosphate	natural abundance	20 (±2.0) mM
9	sodium chloride	natural abundance	100 (±10.0) mM
10	Phosphoprotein(127-163)	natural abundance	300 (±30.0) uM

19 2D 1H-13C HSQC

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
7	Phosphoprotein(127-163) [U-13C; U-15N]	[U-13C; U-15N]	300 (±30.0) uM
8	sodium phosphate	natural abundance	20 (±2.0) mM
9	sodium chloride	natural abundance	100 (±10.0) mM
10	Phosphoprotein(127-163)	natural abundance	300 (±30.0) uM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34513_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34513_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	OK	482		100.0 (chain: 1, length: 45)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 45, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 278
  - ¹³C chemical shifts: 166
  - ¹⁵N chemical shifts: 38
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	233	220	94.4
¹³C chemical shifts	178	166	93.3
¹⁵N chemical shifts	49	38	77.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	95	87	91.6
¹³C chemical shifts	90	79	87.8
¹⁵N chemical shifts	44	36	81.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	138	133	96.4
¹³C chemical shifts	88	87	98.9
¹⁵N chemical shifts	5	2	40.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	29	29	100.0
¹³C chemical shifts	29	29	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	2	1	50.0
¹³C chemical shifts	2	1	50.0

Keywords Coiled-coil, Phosphoprotein, RNA dependent RNA polymerase cofactor, Tetramer, VIRAL PROTEIN

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Found 1 Document (0.588 seconds)

BMRB: 34513

Sample #1

Sample #2

Sample #3

Related entities 1. Phosphoprotein, : 1 : 1 : 14 entities Detail

Interaction partners 1. Phosphoprotein, : 4 interactors Detail

Experiments performed 19 experiments Detail

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Chemical shift validation 3 contents Detail