NMR solution structure of unbound recombinant human Nerve Growth Factor (rhNGF)
SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVR
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS15:SG | 1:CYS80:SG |
2 | disulfide | sing | 1:CYS58:SG | 1:CYS108:SG |
3 | disulfide | sing | 1:CYS68:SG | 1:CYS110:SG |
4 | disulfide | sing | 1:CYS15:SG | 1:CYS80:SG |
5 | disulfide | sing | 1:CYS58:SG | 1:CYS108:SG |
6 | disulfide | sing | 1:CYS68:SG | 1:CYS110:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 82.1 % (1119 of 1363) | 91.8 % (641 of 698) | 67.7 % (361 of 533) | 88.6 % (117 of 132) |
Backbone | 83.2 % (584 of 702) | 96.7 % (232 of 240) | 70.3 % (244 of 347) | 93.9 % (108 of 115) |
Sidechain | 82.9 % (640 of 772) | 89.3 % (409 of 458) | 74.7 % (222 of 297) | 52.9 % (9 of 17) |
Aromatic | 27.5 % (38 of 138) | 50.7 % (35 of 69) | 0.0 % (0 of 66) | 100.0 % (3 of 3) |
Methyl | 99.2 % (119 of 120) | 100.0 % (60 of 60) | 98.3 % (59 of 60) |
1. entity 1
SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7, Details 0.1 mM [U-99% 15N] Nerve Growth Factor, 50 mM HEPES, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Nerve Growth Factor | [U-99% 15N] | 0.1 mM | |
2 | HEPES | natural abundance | 50 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7, Details 0.1 mM [U-99% 13C; U-99% 15N] Nerve Growth Factor, 50 mM HEPES, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | Nerve Growth Factor | [U-99% 13C; U-99% 15N] | 0.1 mM | |
4 | HEPES | natural abundance | 50 mM |