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BMRB: 34516


34516
6P19
Parallel 17-mer DNA G-quadruplex
Authors
Srb, P., Curtis, C., Veverka, V.
Assembly
DNA (5'-D(*GP*GP*GP*TP*GP*GP*GP*AP*AP*GP*GP*GP*TP*GP*GP*GP*A)-3')
Entity
1. DNA (5'-D(*GP*GP*GP*TP*GP*GP*GP*AP*AP*GP*GP*GP*TP*GP*GP*GP*A)-3') (polymer, Thiol state: not present), 17 monomers, 5516.490 Da Detail

GGGTGGGAAG GGTGGGA


Formula weight
5516.49 Da
Source organism
synthetic construct
Exptl. method
solution NMR
Data set
assigned_chemical_shifts, spectral_peak_list
Chem. Shift Complete
Sequence coverage: 88.2 %, Completeness: 74.8 %, Completeness (bb): 73.9 % Detail
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Polymer type: polydeoxyribonucleotide

Total1H
All74.8 % (116 of 155)74.8 % (116 of 155)
Suger, PO473.9 % (88 of 119)73.9 % (88 of 119)
Nucleobase77.8 % (28 of 36)77.8 % (28 of 36)
Aromatic79.4 % (27 of 34)79.4 % (27 of 34)
Methyl50.0 % (1 of 2)50.0 % (1 of 2)

1. entity 1

GGGTGGGAAG GGTGGGA

Show sample condition
Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 0.8 mM 5'-D(*GP*GP*GP*TP*GP*GP*GP*AP*AP*GP*GP*GP*TP*GP*GP*GP*A)-3', 25 mM deuterated TRIS, 200 mM potassium chloride, 1 mM magnesium chloride, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
15'-D(*GP*GP*GP*TP*GP*GP*GP*AP*AP*GP*GP*GP*TP*GP*GP*GP*A)-3'natural abundance0.8 mM
2TRISdeuterated25 mM
3potassium chloridenatural abundance200 mM
4magnesium chloridenatural abundance1 mM

Release date
2020-07-13
Citation
Overlapping but distinct: a new model for G-quadruplex biochemical specificity
Volek, M., Kolesnikova, S., Svehlova, K., Srb, P., Sgallova, R., Streckerova, T., Redondo, J.A., Veverka, V., Curtis, E.A.
Nucleic Acids Res. (2021), 49, 1816-1827, PubMed 33544841 , DOI 10.1093/nar/gkab037 ,
Related entities 1. DNA (5'-D(*GP*GP*GP*TP*GP*GP*GP*AP*AP*GP*GP*GP*TP*GP*GP*GP*A)-3'), : 1 entities Detail
More details for 1 related entities
Experiments performed 3 experiments Detail
Chemical shift validation 3 contents Detail
Keywords DNA, G-quaruplex