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Found 1 Document (0.379 seconds)

BMRB: 34519

6Z40

NMR solution structure of the carbohydrate-binding module family 5 (CBM5) from Cellvibrio japonicus CjLPMO10A

Authors

Madland, E., Aachmann, F.L., Courtade, G.

Assembly

Carbohydrate binding protein, putative, cpb33A

Entity

1. Carbohydrate binding protein, putative, cpb33A (polymer), 67 monomers, 7620.110 Da Detail

MDTCATLPSW DASTVYTNPQ QVKHNSKRYQ ANYWTQNQNP STNSGQYGPW LDLGNCVTSG AHHHHHH

Formula weight

7620.11 Da

Source organism

Cellvibrio japonicus Ueda107

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.6 %, Completeness: 78.5 %, Completeness (bb): 86.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	78.5 % (595 of 758)	79.5 % (310 of 390)	74.7 % (215 of 288)	87.5 % (70 of 80)
Backbone	86.3 % (340 of 394)	87.3 % (117 of 134)	85.8 % (169 of 197)	85.7 % (54 of 63)
Sidechain	72.6 % (310 of 427)	75.4 % (193 of 256)	65.6 % (101 of 154)	94.1 % (16 of 17)
Aromatic	14.6 % (14 of 96)	14.6 % (7 of 48)	8.9 % (4 of 45)	100.0 % (3 of 3)
Methyl	89.1 % (41 of 46)	91.3 % (21 of 23)	87.0 % (20 of 23)

1. entity 1

MDTCATLPSW DASTVYTNPQ QVKHNSKRYQ ANYWTQNQNP STNSGQYGPW LDLGNCVTSG AHHHHHH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5, Details 0.1 mM [U-15N] CBM5, 25 mM sodium phosphate, 10 mM sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	CBM5	[U-15N]	0.1 (±0.05) mM
2	sodium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	10 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5, Details 0.1 mM [U-13C; U-15N] CBM5, 25 mM sodium phosphate, 10 mM sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
4	CBM5	[U-13C; U-15N]	0.1 (±0.05) mM
5	sodium phosphate	natural abundance	25 mM
6	sodium chloride	natural abundance	10 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5, Details 0.1 mM CBM5, 25 mM sodium phosphate, 10 mM sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
7	CBM5	natural abundance	0.1 (±0.05) mM
8	sodium phosphate	natural abundance	25 mM
9	sodium chloride	natural abundance	10 mM

Release date

2020-07-13

Citation

Structural and functional variation of chitin-binding domains of a lytic polysaccharide monooxygenase from Cellvibrio japonicus
Madland, E., Forsberg, Z., Wang, Y., Lindorff-Larsen, K., Niebisch, A., Modregger, J., Eijsink, V.G.H., Aachmann, F.L., Courtade, G.
J. Biol. Chem. (2021), 297, 101084-101084, PubMed 34411561 , DOI 10.1016/j.jbc.2021.101084 , Abstract

Related entities 1. Carbohydrate binding protein, putative, cpb33A, : 1 : 7 entities Detail

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5, Details 0.1 mM [U-15N] CBM5, 25 mM sodium phosphate, 10 mM sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	CBM5	[U-15N]	0.1 (±0.05) mM
2	sodium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	10 mM

2 3D HNCA

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5, Details 0.1 mM [U-13C; U-15N] CBM5, 25 mM sodium phosphate, 10 mM sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
4	CBM5	[U-13C; U-15N]	0.1 (±0.05) mM
5	sodium phosphate	natural abundance	25 mM
6	sodium chloride	natural abundance	10 mM

3 3D HN(CO)CA

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
4	CBM5	[U-13C; U-15N]	0.1 (±0.05) mM
5	sodium phosphate	natural abundance	25 mM
6	sodium chloride	natural abundance	10 mM

4 3D HNCO

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
4	CBM5	[U-13C; U-15N]	0.1 (±0.05) mM
5	sodium phosphate	natural abundance	25 mM
6	sodium chloride	natural abundance	10 mM

5 3D CBCA(CO)NH

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
4	CBM5	[U-13C; U-15N]	0.1 (±0.05) mM
5	sodium phosphate	natural abundance	25 mM
6	sodium chloride	natural abundance	10 mM

6 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
4	CBM5	[U-13C; U-15N]	0.1 (±0.05) mM
5	sodium phosphate	natural abundance	25 mM
6	sodium chloride	natural abundance	10 mM

7 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
4	CBM5	[U-13C; U-15N]	0.1 (±0.05) mM
5	sodium phosphate	natural abundance	25 mM
6	sodium chloride	natural abundance	10 mM

8 3D HCCH-TOCSY

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
4	CBM5	[U-13C; U-15N]	0.1 (±0.05) mM
5	sodium phosphate	natural abundance	25 mM
6	sodium chloride	natural abundance	10 mM

9 3D 1H-13C NOESY aliphatic

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
4	CBM5	[U-13C; U-15N]	0.1 (±0.05) mM
5	sodium phosphate	natural abundance	25 mM
6	sodium chloride	natural abundance	10 mM

10 3D 1H-13C NOESY aromatic

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
4	CBM5	[U-13C; U-15N]	0.1 (±0.05) mM
5	sodium phosphate	natural abundance	25 mM
6	sodium chloride	natural abundance	10 mM

11 3D 1H-15N NOESY

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	CBM5	[U-15N]	0.1 (±0.05) mM
2	sodium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	10 mM

12 2D 1H-1H NOESY

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5, Details 0.1 mM CBM5, 25 mM sodium phosphate, 10 mM sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
7	CBM5	natural abundance	0.1 (±0.05) mM
8	sodium phosphate	natural abundance	25 mM
9	sodium chloride	natural abundance	10 mM

13 3D HN(CA)CO

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
4	CBM5	[U-13C; U-15N]	0.1 (±0.05) mM
5	sodium phosphate	natural abundance	25 mM
6	sodium chloride	natural abundance	10 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34519_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34519_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	631		89.6 (chain: 1, length: 67)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 67, Sequence coverage: 89.6%
- Total number of assignments
  - ¹H chemical shifts: 349
  - ¹³C chemical shifts: 212
  - ¹⁵N chemical shifts: 70
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	390	305	78.2
¹³C chemical shifts	288	212	73.6
¹⁵N chemical shifts	80	70	87.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	134	116	86.6
¹³C chemical shifts	134	113	84.3
¹⁵N chemical shifts	63	54	85.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	256	189	73.8
¹³C chemical shifts	154	99	64.3
¹⁵N chemical shifts	17	16	94.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	24	22	91.7
¹³C chemical shifts	24	21	87.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	48	7	14.6
¹³C chemical shifts	45	4	8.9
¹⁵N chemical shifts	3	3	100.0

Keywords SUGAR BINDING PROTEIN, cbm, chitin

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Found 1 Document (0.379 seconds)

BMRB: 34519

Sample #1

Sample #2

Sample #3

Related entities 1. Carbohydrate binding protein, putative, cpb33A, : 1 : 7 entities Detail

Experiments performed 13 experiments Detail

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Chemical shift validation 3 contents Detail