BMRBj - BMREntryMaster

Found 1 Document (5.247 seconds)

BMRB: 34537

6TVM

TFIIS N-terminal domain (TND) from human LEDGF/p75

Authors

Assembly

PC4 and SFRS1-interacting protein

Entity

1. PC4 and SFRS1-interacting protein (polymer), 92 monomers, 10632.25 Da Detail

SNAASETSMD SRLQRIHAEI KNSLKIDNLD VNRCIEALDE LASLQVTMQQ AQKHTEMITT LKKIRRFKVS QVIMEKSTML YNKFKNMFLV GE

Formula weight

10632.25 Da

Source organism

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 93.6 %, Completeness (bb): 95.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.6 % (1046 of 1117)	93.7 % (547 of 584)	94.3 % (400 of 424)	90.8 % (99 of 109)
Backbone	95.1 % (525 of 552)	94.1 % (174 of 185)	95.3 % (262 of 275)	96.7 % (89 of 92)
Sidechain	93.0 % (610 of 656)	93.5 % (373 of 399)	94.6 % (227 of 240)	58.8 % (10 of 17)
Aromatic	82.6 % (38 of 46)	100.0 % (23 of 23)	65.2 % (15 of 23)
Methyl	96.3 % (104 of 108)	96.3 % (52 of 54)	96.3 % (52 of 54)

1. entity 1

SNAASETSMD SRLQRIHAEI KNSLKIDNLD VNRCIEALDE LASLQVTMQQ AQKHTEMITT LKKIRRFKVS QVIMEKSTML YNKFKNMFLV GE

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.6 mM [U-13C; U-15N] LEDGF/p75, 25 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	LEDGF/p75	[U-13C; U-15N]		0.6 mM
2	TRIS	[U-2H]		25 mM
3	sodium chloride	natural abundance		200 mM
4	TCEP	natural abundance		1 mM

Release date

2020-08-16

Citation

A ubiquitous disordered protein interaction module orchestrates transcription elongation
Cermakova, K., Demeulemeester, J., Lux, V., Nedomova, M., Goldman, S.R., Smith, E.A., Srb, P., Hexnerova, R., Fabry, M., Madlikova, M., Horejsi, M., De Rijck, J., Debyser, Z., Adelman, K., Hodges, H.C., Veverka, V.
Science (2021), 374, 1113-1121, PubMed 34822292 , DOI 10.1126/science.abe2913 , Abstract

Related entities 1. PC4 and SFRS1-interacting protein, : 1 : 1 : 28 : 28 entities Detail

Interaction partners 1. PC4 and SFRS1-interacting protein, : 13 interactors Detail

More details for 13 interactors identified by 3 citations

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.6 mM [U-13C; U-15N] LEDGF/p75, 25 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	LEDGF/p75	[U-13C; U-15N]		0.6 mM
2	TRIS	[U-2H]		25 mM
3	sodium chloride	natural abundance		200 mM
4	TCEP	natural abundance		1 mM

2 2D 1H-13C HSQC

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.6 mM [U-13C; U-15N] LEDGF/p75, 25 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	LEDGF/p75	[U-13C; U-15N]		0.6 mM
2	TRIS	[U-2H]		25 mM
3	sodium chloride	natural abundance		200 mM
4	TCEP	natural abundance		1 mM

3 3D HNCACB

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.6 mM [U-13C; U-15N] LEDGF/p75, 25 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	LEDGF/p75	[U-13C; U-15N]		0.6 mM
2	TRIS	[U-2H]		25 mM
3	sodium chloride	natural abundance		200 mM
4	TCEP	natural abundance		1 mM

4 3D CBCA(CO)NH

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.6 mM [U-13C; U-15N] LEDGF/p75, 25 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	LEDGF/p75	[U-13C; U-15N]		0.6 mM
2	TRIS	[U-2H]		25 mM
3	sodium chloride	natural abundance		200 mM
4	TCEP	natural abundance		1 mM

5 3D HNCO

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.6 mM [U-13C; U-15N] LEDGF/p75, 25 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	LEDGF/p75	[U-13C; U-15N]		0.6 mM
2	TRIS	[U-2H]		25 mM
3	sodium chloride	natural abundance		200 mM
4	TCEP	natural abundance		1 mM

6 3D HCCH-TOCSY

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.6 mM [U-13C; U-15N] LEDGF/p75, 25 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	LEDGF/p75	[U-13C; U-15N]		0.6 mM
2	TRIS	[U-2H]		25 mM
3	sodium chloride	natural abundance		200 mM
4	TCEP	natural abundance		1 mM

7 3D HBHA(CO)NH

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.6 mM [U-13C; U-15N] LEDGF/p75, 25 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	LEDGF/p75	[U-13C; U-15N]		0.6 mM
2	TRIS	[U-2H]		25 mM
3	sodium chloride	natural abundance		200 mM
4	TCEP	natural abundance		1 mM

8 3D HN(CO)CA

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.6 mM [U-13C; U-15N] LEDGF/p75, 25 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	LEDGF/p75	[U-13C; U-15N]		0.6 mM
2	TRIS	[U-2H]		25 mM
3	sodium chloride	natural abundance		200 mM
4	TCEP	natural abundance		1 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34537_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34537_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1189		97.8 (chain: 1, length: 92)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 92, Sequence coverage: 97.8%
- Total number of assignments
  - ¹H chemical shifts: 687
  - ¹³C chemical shifts: 405
  - ¹⁵N chemical shifts: 97
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords TRANSCRIPTION, chromatin reader