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Found 1 Document (0.477 seconds)

BMRB: 34555

7AAF

Structural evolution of the tissue-specific U2AF2 paralog and alternative splicing factor LS2

Authors

Kawale, A.A., Kang, H.S., Sattler, M.

Assembly

U2 snRNP auxiliary factor large subunit

Entity

1. U2 snRNP auxiliary factor large subunit (polymer), 81 monomers, 8898.167 Da Detail

AMGNKIYVGG LPTCLNQDQV KELLQSFGEL KGLNLVMDTN TNLNKGFAFF EYCDPSVTDH AIAGLHGMLL GDRRLVVQRS I

Formula weight

8898.167 Da

Source organism

Drosophila melanogaster

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 89.5 %, Completeness (bb): 85.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.5 % (838 of 936)	96.9 % (467 of 482)	78.5 % (284 of 362)	94.6 % (87 of 92)
Backbone	85.1 % (410 of 482)	98.8 % (167 of 169)	70.9 % (166 of 234)	97.5 % (77 of 79)
Sidechain	95.1 % (500 of 526)	95.8 % (300 of 313)	95.0 % (190 of 200)	76.9 % (10 of 13)
Aromatic	93.8 % (60 of 64)	93.8 % (30 of 32)	93.8 % (30 of 32)
Methyl	91.0 % (91 of 100)	92.0 % (46 of 50)	90.0 % (45 of 50)

1. entity 1

AMGNKIYVGG LPTCLNQDQV KELLQSFGEL KGLNLVMDTN TNLNKGFAFF EYCDPSVTDH AIAGLHGMLL GDRRLVVQRS I

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.5 mM 13C 15N LS2 RRM2, 0.02 mM 13C 15N potassium phosphate pH 6.5, 0.05 mM 13C 15N sodium chloride, 0.002 mM 13C 15N DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	LS2 RRM2	[U-13C; U-15N]	0.5 mM
2	potassium phosphate pH 6.5	natural abundance	0.02 mM
3	sodium chloride	natural abundance	0.05 mM
4	DTT	natural abundance	0.002 mM

Release date

2020-10-18

Citation

Structural evolution of the tissue-specific U2AF2 paralog and alternative splicing factor LS2
Kawale, A.A., Kang, H.S., Sattler, M.

Related entities 1. U2 snRNP auxiliary factor large subunit, : 1 : 2 : 365 entities Detail

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE 800 - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.5 mM 13C 15N LS2 RRM2, 0.02 mM 13C 15N potassium phosphate pH 6.5, 0.05 mM 13C 15N sodium chloride, 0.002 mM 13C 15N DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	LS2 RRM2	[U-13C; U-15N]	0.5 mM
2	potassium phosphate pH 6.5	natural abundance	0.02 mM
3	sodium chloride	natural abundance	0.05 mM
4	DTT	natural abundance	0.002 mM

2 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE 800 - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	LS2 RRM2	[U-13C; U-15N]	0.5 mM
2	potassium phosphate pH 6.5	natural abundance	0.02 mM
3	sodium chloride	natural abundance	0.05 mM
4	DTT	natural abundance	0.002 mM

3 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE 800 - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	LS2 RRM2	[U-13C; U-15N]	0.5 mM
2	potassium phosphate pH 6.5	natural abundance	0.02 mM
3	sodium chloride	natural abundance	0.05 mM
4	DTT	natural abundance	0.002 mM

4 3D HNCACB

Instrument

Bruker AVANCE 750 - 750 MHz

Sample

#	Name	Isotope labeling	Concentration
1	LS2 RRM2	[U-13C; U-15N]	0.5 mM
2	potassium phosphate pH 6.5	natural abundance	0.02 mM
3	sodium chloride	natural abundance	0.05 mM
4	DTT	natural abundance	0.002 mM

5 3D HNCA

Instrument

Bruker AVANCE 750 - 750 MHz

Sample

#	Name	Isotope labeling	Concentration
1	LS2 RRM2	[U-13C; U-15N]	0.5 mM
2	potassium phosphate pH 6.5	natural abundance	0.02 mM
3	sodium chloride	natural abundance	0.05 mM
4	DTT	natural abundance	0.002 mM

6 3D 1H-13C NOESY aliphatic

Instrument

Bruker AVANCE 800 - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	LS2 RRM2	[U-13C; U-15N]	0.5 mM
2	potassium phosphate pH 6.5	natural abundance	0.02 mM
3	sodium chloride	natural abundance	0.05 mM
4	DTT	natural abundance	0.002 mM

7 3D 1H-15N NOESY

Instrument

Bruker AVANCE 900 - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	LS2 RRM2	[U-13C; U-15N]	0.5 mM
2	potassium phosphate pH 6.5	natural abundance	0.02 mM
3	sodium chloride	natural abundance	0.05 mM
4	DTT	natural abundance	0.002 mM

8 3D 1H-13C NOESY aromatic

Instrument

Bruker AVANCE 800 - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	LS2 RRM2	[U-13C; U-15N]	0.5 mM
2	potassium phosphate pH 6.5	natural abundance	0.02 mM
3	sodium chloride	natural abundance	0.05 mM
4	DTT	natural abundance	0.002 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34555_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34555_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	908		100.0 (chain: 1, length: 81)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 81, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 552
  - ¹³C chemical shifts: 269
  - ¹⁵N chemical shifts: 87
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	482	464	96.3
¹³C chemical shifts	362	269	74.3
¹⁵N chemical shifts	92	87	94.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	169	167	98.8
¹³C chemical shifts	162	81	50.0
¹⁵N chemical shifts	79	77	97.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	313	297	94.9
¹³C chemical shifts	200	188	94.0
¹⁵N chemical shifts	13	10	76.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	53	44	83.0
¹³C chemical shifts	53	44	83.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	32	30	93.8
¹³C chemical shifts	32	30	93.8

Keywords G quadruplex, LS2, RNA BINDING PROTEIN, RNA-protein interactions, RRM RNA binding domain, U2AF, alternative splicing, structural biology

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Found 1 Document (0.477 seconds)

BMRB: 34555

Sample

Related entities 1. U2 snRNP auxiliary factor large subunit, : 1 : 2 : 365 entities Detail

Experiments performed 8 experiments Detail

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Chemical shift validation 3 contents Detail