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BMRB: 34571

G-quadruplex with V-shaped loop from the first repeat of KCNN4 minisatellite
Authors
Vianney, Y.M., Weisz, K.
Assembly
DNA (5'-D(*GP*GP*TP*CP*TP*GP*AP*GP*GP*GP*AP*GP*AP*GP*GP*GP*GP*CP*TP*GP*GP*GP*T)-3')
Entity
1. DNA (5'-D(*GP*GP*TP*CP*TP*GP*AP*GP*GP*GP*AP*GP*AP*GP*GP*GP*GP*CP*TP*GP*GP*GP*T)-3') (polymer, Thiol state: not present), 23 monomers, 7361.650 Da Detail

GGTCTGAGGG AGAGGGGCTG GGT


Formula weight
7361.65 Da
Source organism
synthetic construct
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 63.3 %, Completeness (bb): 57.1 % Detail
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Polymer type: polydeoxyribonucleotide

Total1H
All63.3 % (136 of 215)63.3 % (136 of 215)
Suger, PO457.1 % (92 of 161)57.1 % (92 of 161)
Nucleobase81.5 % (44 of 54)81.5 % (44 of 54)
Aromatic87.0 % (40 of 46)87.0 % (40 of 46)
Methyl100.0 % (4 of 4)100.0 % (4 of 4)

1. DNA (5'-D(*GP*GP*TP*CP*TP*GP*AP*GP*GP*GP*AP*GP*AP*GP*GP*GP*GP*CP*TP*GP*GP*GP*T)-3')

GGTCTGAGGG AGAGGGGCTG GGT

Show sample condition
Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.9 mM No DNA, 90% H2O/10% D2O


#NameIsotope labelingTypeConcentration
1DNAnatural abundance0.9 mM
Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.9 mM No DNA, 100% D2O


#NameIsotope labelingTypeConcentration
2DNAnatural abundance0.9 mM

Release date
2020-11-04
Citation
First Tandem Repeat of a Potassium Channel KCNN4 Minisatellite Folds into a V-Loop G-Quadruplex Structure
Vianney, Y.M., Weisz, K.
Biochemistry (2021), 60, 1337-1346, PubMed 33844501 , DOI 10.1021/acs.biochem.1c00043 ,
Experiments performed 4 experiments Detail
Chemical shift validation 3 contents Detail
Keywords DNA, G-Quadruplex, V-loop