Solution structure of the fluorogen-activating protein FAST in complex with the ligand N871b
MEHVAFGSED IENTLAKMDD GQLDGLAFGA IQLDGDGNIL QYNAAEGDIT GRDPKQVIGK NFFKDVAPGT DSPEFYGKFK EGVASGNLNT MFEWMIPTSR GPTKVKVHMK KALSGDSYWV FVKRVKLAAA LEHHHHHH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 94.9 % (1507 of 1588) | 96.2 % (789 of 820) | 93.1 % (577 of 620) | 95.3 % (141 of 148) |
Backbone | 97.1 % (794 of 818) | 96.5 % (276 of 286) | 97.7 % (390 of 399) | 96.2 % (128 of 133) |
Sidechain | 93.3 % (833 of 893) | 96.1 % (513 of 534) | 89.2 % (307 of 344) | 86.7 % (13 of 15) |
Aromatic | 71.2 % (114 of 160) | 85.0 % (68 of 80) | 56.4 % (44 of 78) | 100.0 % (2 of 2) |
Methyl | 100.0 % (132 of 132) | 100.0 % (66 of 66) | 100.0 % (66 of 66) |
1. entity 1
MEHVAFGSED IENTLAKMDD GQLDGLAFGA IQLDGDGNIL QYNAAEGDIT GRDPKQVIGK NFFKDVAPGT DSPEFYGKFK EGVASGNLNT MFEWMIPTSR GPTKVKVHMK KALSGDSYWV FVKRVKLAAA LEHHHHHHSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.85 mM [U-13C; U-15N] FAST, 0.90 mM N87, 20 mM sodium phosphate, 20 mM sodium chloride, 0.01 % sodium azide, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | FAST | [U-13C; U-15N] | 0.85 (±0.05) mM | |
2 | N87 | natural abundance | 0.90 (±0.01) mM | |
3 | sodium phosphate | natural abundance | 20 mM | |
4 | sodium chloride | natural abundance | 20 mM | |
5 | sodium azide | natural abundance | 0.01 % |