NMR2 structure of TRIM24-BD in complex with a precursor of IACS-9571
TPIDKRKCER LLLFLYCHEM SLAFQDPVPL TVPDYYKIIK NPMDLSTIKK RLQEDYSMYS KPEDFVADFR LIFQNCAEFN EPDSEVANAG IKLENYFEEL LKNLYP
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 5.6 % (39 of 695) | 5.6 % (39 of 695) |
Backbone | 2.9 % (6 of 205) | 2.9 % (6 of 205) |
Sidechain | 6.7 % (33 of 490) | 6.7 % (33 of 490) |
Aromatic | 0.0 % (0 of 65) | 0.0 % (0 of 65) |
Methyl | 27.8 % (15 of 54) | 27.8 % (15 of 54) |
1. entity 1
TPIDKRKCER LLLFLYCHEM SLAFQDPVPL TVPDYYKIIK NPMDLSTIKK RLQEDYSMYS KPEDFVADFR LIFQNCAEFN EPDSEVANAG IKLENYFEEL LKNLYPSolvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 303 (±0.25) K, pH 7.4 (±0.05), Details 1.1 mM [U-13C; U-15N] TRIM24-BD, 50 mM [U-2H] HEPES, 100 mM sodium chloride, 1 mM TCEP, 10 % [U-2H] D2O, 1.1 mM N-{6-[3-(4-Aminobutoxy)-5-propoxyphenoxy]-1,3-dimethyl-2-oxo-2,3-dihydro-1H-1,3-benzodiazol-5-yl}-3,4-dimethoxybenzene-1-sulfonamide, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TRIM24-BD | [U-13C; U-15N] | 1.1 mM | |
2 | HEPES | [U-2H] | 50 mM | |
3 | sodium chloride | natural abundance | 100 mM | |
4 | TCEP | natural abundance | 1 mM | |
5 | D2O | [U-2H] | 10 % | |
6 | entity_T52 | natural abundance | 1.1 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 303 (±0.25) K, pH 7.4 (±0.05), Details 50 mM [U-2H] HEPES, 100 mM sodium chloride, 1 mM TCEP, 10 % [U-2H] D2O, 1.1 % N-{6-[3-(4-Aminobutoxy)-5-propoxyphenoxy]-1,3-dimethyl-2-oxo-2,3-dihydro-1H-1,3-benzodiazol-5-yl}-3,4-dimethoxybenzene-1-sulfonamide, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | HEPES | [U-2H] | 50 mM | |
8 | sodium chloride | natural abundance | 100 mM | |
9 | TCEP | natural abundance | 1 mM | |
10 | D2O | [U-2H] | 10 % | |
11 | entity_T52 | natural abundance | 1.1 % |