NMR structure of an optimized version of the first TPR domain of the human SPAG1 protein
GPHMDYLATR EKEKGNEAFN SGDYEEAVMY YTRSISALPT VVAYNNRAQA YIKLQNWNSA EQDCEKVLEL EPGNVKALLR RATAYKHQNK LREAREDLKK VLKVEPDNDL AKKTLSEVER DLKNSE
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 97.4 % (1459 of 1498) | 98.5 % (773 of 785) | 96.1 % (546 of 568) | 96.6 % (140 of 145) |
Backbone | 97.2 % (727 of 748) | 98.0 % (247 of 252) | 96.3 % (360 of 374) | 98.4 % (120 of 122) |
Sidechain | 96.4 % (841 of 872) | 97.2 % (518 of 533) | 95.9 % (303 of 316) | 87.0 % (20 of 23) |
Aromatic | 86.0 % (74 of 86) | 93.0 % (40 of 43) | 78.6 % (33 of 42) | 100.0 % (1 of 1) |
Methyl | 100.0 % (128 of 128) | 100.0 % (64 of 64) | 100.0 % (64 of 64) |
1. entity 1
GPHMDYLATR EKEKGNEAFN SGDYEEAVMY YTRSISALPT VVAYNNRAQA YIKLQNWNSA EQDCEKVLEL EPGNVKALLR RATAYKHQNK LREAREDLKK VLKVEPDNDL AKKTLSEVER DLKNSESolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.4, Details 1 mM [U-13C; U-15N] SPAG1-TPR1, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | SPAG1-TPR1 | [U-13C; U-15N] | 1 (±0.1) mM | |
2 | NaPi | natural abundance | 10 mM | |
3 | NaCl | natural abundance | 150 mM |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.4, Details 1 mM [U-13C; U-15N] SPAG1-TPR1, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | SPAG1-TPR1 | [U-13C; U-15N] | 1 (±0.1) mM | |
2 | NaPi | natural abundance | 10 mM | |
3 | NaCl | natural abundance | 150 mM |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.4, Details 1 mM [U-13C; U-15N] SPAG1-TPR1, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | SPAG1-TPR1 | [U-13C; U-15N] | 1 (±0.1) mM | |
2 | NaPi | natural abundance | 10 mM | |
3 | NaCl | natural abundance | 150 mM |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.4, Details 1 mM [U-13C; U-15N] SPAG1-TPR1, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | SPAG1-TPR1 | [U-13C; U-15N] | 1 (±0.1) mM | |
2 | NaPi | natural abundance | 10 mM | |
3 | NaCl | natural abundance | 150 mM |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.4, Details 1 mM [U-13C; U-15N] SPAG1-TPR1, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | SPAG1-TPR1 | [U-13C; U-15N] | 1 (±0.1) mM | |
2 | NaPi | natural abundance | 10 mM | |
3 | NaCl | natural abundance | 150 mM |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.4, Details 1 mM [U-13C; U-15N] SPAG1-TPR1, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | SPAG1-TPR1 | [U-13C; U-15N] | 1 (±0.1) mM | |
2 | NaPi | natural abundance | 10 mM | |
3 | NaCl | natural abundance | 150 mM |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.4, Details 1 mM [U-13C; U-15N] SPAG1-TPR1, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | SPAG1-TPR1 | [U-13C; U-15N] | 1 (±0.1) mM | |
2 | NaPi | natural abundance | 10 mM | |
3 | NaCl | natural abundance | 150 mM |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.4, Details 1 mM [U-13C; U-15N] SPAG1-TPR1, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | SPAG1-TPR1 | [U-13C; U-15N] | 1 (±0.1) mM | |
2 | NaPi | natural abundance | 10 mM | |
3 | NaCl | natural abundance | 150 mM |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.4, Details 1 mM [U-13C; U-15N] SPAG1-TPR1, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | SPAG1-TPR1 | [U-13C; U-15N] | 1 (±0.1) mM | |
2 | NaPi | natural abundance | 10 mM | |
3 | NaCl | natural abundance | 150 mM |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.4, Details 1 mM [U-13C; U-15N] SPAG1-TPR1, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | SPAG1-TPR1 | [U-13C; U-15N] | 1 (±0.1) mM | |
2 | NaPi | natural abundance | 10 mM | |
3 | NaCl | natural abundance | 150 mM |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.4, Details 1 mM [U-13C; U-15N] SPAG1-TPR1, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | SPAG1-TPR1 | [U-13C; U-15N] | 1 (±0.1) mM | |
2 | NaPi | natural abundance | 10 mM | |
3 | NaCl | natural abundance | 150 mM |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.4, Details 1 mM [U-13C; U-15N] SPAG1-TPR1, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | SPAG1-TPR1 | [U-13C; U-15N] | 1 (±0.1) mM | |
2 | NaPi | natural abundance | 10 mM | |
3 | NaCl | natural abundance | 150 mM |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.4, Details 1 mM [U-13C; U-15N] SPAG1-TPR1, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | SPAG1-TPR1 | [U-13C; U-15N] | 1 (±0.1) mM | |
2 | NaPi | natural abundance | 10 mM | |
3 | NaCl | natural abundance | 150 mM |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.4, Details 1 mM [U-13C; U-15N] SPAG1-TPR1, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | SPAG1-TPR1 | [U-13C; U-15N] | 1 (±0.1) mM | |
2 | NaPi | natural abundance | 10 mM | |
3 | NaCl | natural abundance | 150 mM |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.4, Details 1 mM [U-13C; U-15N] SPAG1-TPR1, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | SPAG1-TPR1 | [U-13C; U-15N] | 1 (±0.1) mM | |
2 | NaPi | natural abundance | 10 mM | |
3 | NaCl | natural abundance | 150 mM |
Properties
Disulfide bonds
NoneOther bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)
NoneNon-standard residues
NoneContent subtype: bmr34586_3.str
Assigned chemical shifts
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 GPHMDYLATREKEKGNEAFNSGDYEEAVMYYTRSISALPTVVAYNNRAQAYIKLQNWNSAEQDCEKVLELEPGNVKALLRRATAYKHQNKLREAREDLKK |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| GPHMDYLATREKEKGNEAFNSGDYEEAVMYYTRSISALPTVVAYNNRAQAYIKLQNWNSAEQDCEKVLELEPGNVKALLRRATAYKHQNKLREAREDLKK -------110-------120------ VLKVEPDNDLAKKTLSEVERDLKNSE |||||||||||||||||||||||||| VLKVEPDNDLAKKTLSEVERDLKNSE
Comp_index_ID | Comp_ID | Atom_ID | CS value (ppm) |
---|---|---|---|
32 | THR | HG1 | 5.66 |
34 | SER | HG | 5.948 |
40 | THR | HG1 | 5.883 |
64 | CYS | HG | 3.018 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 785 | 779 | 99.2 |
13C chemical shifts | 568 | 551 | 97.0 |
15N chemical shifts | 145 | 142 | 97.9 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 252 | 251 | 99.6 |
13C chemical shifts | 252 | 244 | 96.8 |
15N chemical shifts | 122 | 121 | 99.2 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 533 | 528 | 99.1 |
13C chemical shifts | 316 | 307 | 97.2 |
15N chemical shifts | 23 | 21 | 91.3 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 66 | 66 | 100.0 |
13C chemical shifts | 66 | 66 | 100.0 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 43 | 40 | 93.0 |
13C chemical shifts | 42 | 33 | 78.6 |
15N chemical shifts | 1 | 1 | 100.0 |