BMRB: 34609

NMR structure of GIPC1-GH2 domain

Authors

Barthe, P., Roumestand, C.

Assembly

PDZ domain-containing protein GIPC1

Entity

1. PDZ domain-containing protein GIPC1 (polymer, Thiol state: not present), 83 monomers, 9172.122 Da Detail

GAMGDLPSAF EEKAIEKVDD LLESYMGIRD TELAATMVEL GKDKRNPDEL AEALDERLGD FAFPDEFVFD VWGAIGDAKV GRY

Formula weight

9172.122 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.4 %, Completeness: 79.9 %, Completeness (bb): 93.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	79.9 % (748 of 936)	92.1 % (443 of 481)	61.2 % (226 of 369)	91.9 % (79 of 86)
Backbone	93.7 % (461 of 492)	94.2 % (161 of 171)	92.5 % (223 of 241)	96.2 % (77 of 80)
Sidechain	68.8 % (357 of 519)	91.0 % (282 of 310)	36.0 % (73 of 203)	33.3 % (2 of 6)
Aromatic	35.9 % (28 of 78)	71.8 % (28 of 39)	0.0 % (0 of 38)	0.0 % (0 of 1)
Methyl	59.1 % (52 of 88)	97.7 % (43 of 44)	20.5 % (9 of 44)

1. entity 1

GAMGDLPSAF EEKAIEKVDD LLESYMGIRD TELAATMVEL GKDKRNPDEL AEALDERLGD FAFPDEFVFD VWGAIGDAKV GRY

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2, Details 1.0 mM [U-15N] GIPC1-GH2, 20 mM TRIS, 0.1 mM EDTA, 0.1 mM PMSF, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	GIPC1-GH2	[U-15N]		1.0 mM
2	TRIS	natural abundance		20 mM
3	EDTA	natural abundance		0.1 mM
4	PMSF	natural abundance		0.1 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2, Details 1.0 mM [U-13C; U-15N] GIPC1-GH2, 20 mM TRIS, 0.1 mM EDTA, 0.1 mM PMSF, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
5	GIPC1-GH2	[U-13C; U-15N]		1.0 mM
6	TRIS	natural abundance		20 mM
7	EDTA	natural abundance		0.1 mM
8	PMSF	natural abundance		0.1 mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2, Details 1.0 mM GIPC1-GH2, 20 mM TRIS, 0.1 mM EDTA, 0.1 mM PMSF, 100% D2O

#	Name	Isotope labeling	Type	Concentration
9	GIPC1-GH2	natural abundance		1.0 mM
10	TRIS	natural abundance		20 mM
11	EDTA	natural abundance		0.1 mM
12	PMSF	natural abundance		0.1 mM

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Release date

2021-03-28

Citation

Pressure and Chemical Unfolding of an α-Helical Bundle Protein: The GH2 Domain of the Protein Adaptor GIPC1
Dubois, C., Planelles-Herrero, V.J., Tillatte-Tripodi, C., Delbecq, S., Mammri, L., Sirkia, E.M., Ropars, V., Roumestand, C., Barthe, P.
Int. J. Mol. Sci. (2021), 22, 3597-3597, PubMed 33808390 , DOI 10.3390/ijms22073597 , Abstract

Related entities 1. PDZ domain-containing protein GIPC1, : 1 : 9 : 17 entities Detail

Interaction partners 1. PDZ domain-containing protein GIPC1, : 8 interactors Detail

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail