Search

Solution structure of toll like receptor 1 (TLR1) TIR domain
Authors
Mineev, K.S., Lushpa, V.A., Goncharuk, M.V.
Assembly
Toll-like receptor 1 (E.C.3.2.2.6)
Entity
1. Toll-like receptor 1 (E.C.3.2.2.6) (polymer), 163 monomers, 19176.90 Da Detail

SNIPLEELQR NLQFHAFISY SGHDSFWVKN ELLPNLEKEG MQICLHERNF VPGKSIVENI ITCIEKSYKS IFVLSPNFVQ SEWCHYELYF AHHNLFHEGS NSLILILLEP IPQYSIPSSY HKLKSLMARR TYLEWPKEKS KRGLFWANLR AAINIKLTEQ AKK


Formula weight
19176.9 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 98.2 %, Completeness: 92.2 %, Completeness (bb): 93.6 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All92.2 % (1895 of 2056)93.9 % (1009 of 1074)90.8 % (726 of 800)87.9 % (160 of 182)
Backbone93.6 % (900 of 962)92.9 % (300 of 323)94.8 % (459 of 484)91.0 % (141 of 155)
Sidechain90.0 % (1127 of 1252)92.7 % (696 of 751)87.1 % (413 of 474)66.7 % (18 of 27)
Aromatic70.4 % (159 of 226)85.0 % (96 of 113)54.1 % (59 of 109)100.0 % (4 of 4)
Methyl97.7 % (172 of 176)97.7 % (86 of 88)97.7 % (86 of 88)

1. entity 1

SNIPLEELQR NLQFHAFISY SGHDSFWVKN ELLPNLEKEG MQICLHERNF VPGKSIVENI ITCIEKSYKS IFVLSPNFVQ SEWCHYELYF AHHNLFHEGS NSLILILLEP IPQYSIPSSY HKLKSLMARR TYLEWPKEKS KRGLFWANLR AAINIKLTEQ AKK

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3, Details 0.8 mM [U-100% 13C; U-100% 15N] TLR1-TIR, 20 mM PIPES, 25 mM sodium chloride, 3 mM TCEP, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
1TLR1-TIR[U-100% 13C; U-100% 15N]0.8 mM
2PIPESnatural abundance20 mM
3sodium chloridenatural abundance25 mM
4TCEPnatural abundance3 mM

Release date
2021-03-30
Citation
Modulation of Toll-like receptor 1 intracellular domain structure and activity by Zn2+ ions
Lushpa, V.A., Goncharuk, M.V., Lin, C., Zalevsky, A.O., Talyzina, I.A., Luginina, A.P., Vakhrameev, D.D., Shevtsov, M.B., Goncharuk, S.A., Arseniev, A.S., Borshchevskiy, V.I., Wang, X., Mineev, K.S.
Commun. Biol. (2021), 4, 1003-1003, PubMed 34429510 , DOI 10.1038/s42003-021-02532-0 ,
Related entities 1. Toll-like receptor 1 (E.C.3.2.2.6), : 1 : 5 : 177 entities Detail
Interaction partners 1. Toll-like receptor 1 (E.C.3.2.2.6), : 4 interactors Detail
Experiments performed 7 experiments Detail
Chemical shift validation 3 contents Detail
Keywords PROTEIN, SIGNALING PROTEIN, TIR domain, TLR, TLR1, toll like receptor