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BMRB: 34611

Three-quartet c-kit2 G-quadruplex stabilized by a pyrene conjugate
Authors
Peterkova, K., Durnik, I., Marek, R., Plavec, J., Podbevsek, P.
Assembly
c-kit2 py1
Entity
1. c-kit2 py1, entity 1 (polymer, Thiol state: not present), 21 monomers, 6382.018 Da Detail

XGGGCGGGCG CTAGGGAGGG T


2. c-kit2 py1, entity K (non-polymer), 39.098 × 2 Da
Total weight
6460.214 Da
Max. entity weight
6382.018 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 95.2 %, Completeness: 61.7 %, Completeness (bb): 55.0 % Detail
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Polymer type: polydeoxyribonucleotide

Total1H
All61.7 % (116 of 188)61.7 % (116 of 188)
Suger, PO455.0 % (77 of 140)55.0 % (77 of 140)
Nucleobase81.3 % (39 of 48)81.3 % (39 of 48)
Aromatic92.5 % (37 of 40)92.5 % (37 of 40)
Methyl100.0 % (2 of 2)100.0 % (2 of 2)

1. entity 1

XGGGCGGGCG CTAGGGAGGG T

Show sample condition
Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1.0 mM c-kit2_py1, 5 mM potassium phosphate, 20 mM potassion chloride, 90% H2O/10% D2O


#NameIsotope labelingTypeConcentration
1c-kit2_py1natural abundance1.0 (±0.1) mM
2potassium phosphatenatural abundance5 (±0.5) mM
3potassion chloridenatural abundance20 (±1.0) mM

Release date
2021-04-13
Citation
c-kit2 G-quadruplex stabilized via a covalent probe: exploring G-quartet asymmetry
Peterkova, K., Durnik, I., Marek, R., Plavec, J., Podbevsek, P.
Nucleic Acids Res. (2021), 49, 8947-8960, PubMed 34365512 , DOI 10.1093/nar/gkab659 ,
Experiments performed 2 experiments Detail
Chemical shift validation 4 contents Detail
Keywords DNA, G-quadruplex, c-kit2, pyrene