BMRB: 34626

A self-complementary DNA dodecamer duplex contaning 5-hydroxymethylcitosine

Authors

Battistini, F., Dans, P.D., Terrazas, M., Castellazzi, C.L., Portella, G., Labrador, M., Villegas, N., Brun-Heath, I., Gonzalez, C., Orozco, M.

Assembly

DNA (5'-D(*CP*GP*AP*(DH)P*GP*TP*CP*G)-3')

Entity

1. DNA (5'-D(*CP*GP*AP*(DH)P*GP*TP*CP*G)-3') (polymer, Thiol state: not present), 8 monomers, 2520.603 × 2 Da Detail

CGAXGTCG

Total weight

5041.206 Da

Max. entity weight

2520.603 Da

Source organism

synthetic construct

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.5 %, Completeness: 88.2 %, Completeness (bb): 83.7 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	88.2 % (60 of 68)	88.2 % (60 of 68)
Suger, PO4	83.7 % (41 of 49)	83.7 % (41 of 49)
Nucleobase	100.0 % (19 of 19)	100.0 % (19 of 19)
Aromatic	100.0 % (14 of 14)	100.0 % (14 of 14)
Methyl	100.0 % (1 of 1)	100.0 % (1 of 1)

1. entity 1

CGAXGTCG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 7.0, Details 1 mM DNA (5'-D(*CP*GP*AP*(DH)P*GP*TP*CP*G)-3'), 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	DNA (5'-D(*CP*GP*AP*(DH)P*GP*TP*CP*G)-3')	natural abundance		1 mM

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Release date

2021-06-10

Citation

The Impact of the HydroxyMethylCytosine epigenetic signature on DNA structure and function
Battistini, F., Dans, P.D., Terrazas, M., Castellazzi, C.L., Portella, G., Labrador, M., Villegas, N., Brun-Heath, I., Gonzalez, C., Orozco, M.
PLoS Comput. Biol. (2021), 17, e1009547-e1009547, PubMed 34748533 , DOI 10.1371/journal.pcbi.1009547 , Abstract

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail