BMRBj - BMREntryMaster

Found 1 Document (0.702 seconds)

BMRB: 34663

7PMP

Solution structure of Legionella pneumophila LspD

Authors

Portlock, T.J., Garnett, J.A.

Assembly

Type II protein secretion LspD

Entity

1. Type II protein secretion LspD (polymer, Thiol state: not present), 111 monomers, 12275.93 Da Detail

MAHHHHHHVD DDDKMGSKLW NLRNADIRAV IAEVSRITGK NFVIDPRVQG KVSIVSSTPL SSRELYQVFL SVLQVSGYAA IPNGEIIKII PNIDAKTQSP DLLSGMKSPP R

Formula weight

12275.93 Da

Source organism

Legionella pneumophila

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.3 %, Completeness: 83.8 %, Completeness (bb): 85.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.8 % (1081 of 1290)	84.1 % (562 of 668)	83.9 % (421 of 502)	81.7 % (98 of 120)
Backbone	85.1 % (555 of 652)	84.6 % (187 of 221)	85.6 % (280 of 327)	84.6 % (88 of 104)
Sidechain	83.0 % (617 of 743)	83.9 % (375 of 447)	82.9 % (232 of 280)	62.5 % (10 of 16)
Aromatic	52.8 % (38 of 72)	63.9 % (23 of 36)	40.0 % (14 of 35)	100.0 % (1 of 1)
Methyl	96.3 % (131 of 136)	95.6 % (65 of 68)	97.1 % (66 of 68)

1. entity 1

MAHHHHHHVD DDDKMGSKLW NLRNADIRAV IAEVSRITGK NFVIDPRVQG KVSIVSSTPL SSRELYQVFL SVLQVSGYAA IPNGEIIKII PNIDAKTQSP DLLSGMKSPP R

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.0, Details 0.4 mM [U-99% 13C; U-99% 15N] LspD N0, 50 mM sodium phosphate, 50 mM sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	LspD N0	[U-99% 13C; U-99% 15N]	0.4 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM

Release date

2021-09-27

Citation

Solution structure of Legionella pneumophila LspD N0
Portlock, T.J., Garnett, J.A.

Related entities 1. Type II protein secretion LspD, : 1 : 10 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.0, Details 0.4 mM [U-99% 13C; U-99% 15N] LspD N0, 50 mM sodium phosphate, 50 mM sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	LspD N0	[U-99% 13C; U-99% 15N]	0.4 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM

2 3D CBCA(CO)NH

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	LspD N0	[U-99% 13C; U-99% 15N]	0.4 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM

3 3D HNCO

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	LspD N0	[U-99% 13C; U-99% 15N]	0.4 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM

4 3D HCCH-TOCSY

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	LspD N0	[U-99% 13C; U-99% 15N]	0.4 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM

5 3D CCH-TOCSY

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	LspD N0	[U-99% 13C; U-99% 15N]	0.4 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM

6 3D HNCA

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	LspD N0	[U-99% 13C; U-99% 15N]	0.4 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM

7 3D 1H-15N NOESY

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	LspD N0	[U-99% 13C; U-99% 15N]	0.4 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM

8 3D 1H-13C NOESY

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	LspD N0	[U-99% 13C; U-99% 15N]	0.4 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM

9 3D HNCACB

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	LspD N0	[U-99% 13C; U-99% 15N]	0.4 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM

10 3D HN(CO)CA

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	LspD N0	[U-99% 13C; U-99% 15N]	0.4 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM

11 2D 1H-13C HSQC

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	LspD N0	[U-99% 13C; U-99% 15N]	0.4 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM

nullKeywords GspD, Legionella pneumophila, PROTEIN TRANSPORT, Recognition, Type II Secretion System

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Found 1 Document (0.702 seconds)

BMRB: 34663

Sample

Related entities 1. Type II protein secretion LspD, : 1 : 10 entities Detail

Experiments performed 11 experiments Detail

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