Solution structure of the chimeric Nrd1-Nab3 heterodimerization domains
TANTASQQLS LDPKQRSKQI LSNLKKSPPL NLNISLPTDL TSTDPAKQQA ALFQVIAALQ KHFKTNMENV NYDLLQKQVK YIMDSNMLNL PQFQHLPQEE KMSAILAMLN SNSDTALSVP PHDST
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 95.7 % (1421 of 1485) | 96.3 % (750 of 779) | 94.0 % (531 of 565) | 99.3 % (140 of 141) |
Backbone | 98.2 % (719 of 732) | 98.8 % (238 of 241) | 97.3 % (365 of 375) | 100.0 % (116 of 116) |
Sidechain | 93.8 % (824 of 878) | 95.2 % (512 of 538) | 91.4 % (288 of 315) | 96.0 % (24 of 25) |
Aromatic | 55.2 % (32 of 58) | 89.7 % (26 of 29) | 20.7 % (6 of 29) | |
Methyl | 99.3 % (143 of 144) | 98.6 % (71 of 72) | 100.0 % (72 of 72) |
1. entity 1
TANTASQQLS LDPKQRSKQI LSNLKKSPPL NLNISLPTDL TSTDPAKQQA ALFQVIAALQ KHFKTNMENV NYDLLQKQVK YIMDSNMLNL PQFQHLPQEE KMSAILAMLN SNSDTALSVP PHDSTSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.1
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | sodium acetate | natural abundance | 10 mM | |
2 | sodium chloride | natural abundance | 25 mM | |
3 | DTT | natural abundance | 1 mM | |
4 | Nrd1-Nab3 chimera | [U-100% 13C; U-100% 15N] | 1000 uM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.1
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | sodium acetate | natural abundance | 10 mM | |
6 | sodium chloride | natural abundance | 25 mM | |
7 | DTT | natural abundance | 1 mM | |
8 | Nrd1-Nab3 chimera | [U-100% 13C; U-100% 15N] | 1000 uM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.1
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | sodium acetate | natural abundance | 10 mM | |
10 | sodium chloride | natural abundance | 25 mM | |
11 | DTT | natural abundance | 1 mM | |
12 | Nrd1-Nab3 chimera | [13C - methyl (IVL)] | 1000 uM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.1
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | sodium acetate | natural abundance | 10 mM | |
14 | sodium chloride | natural abundance | 25 mM | |
15 | DTT | natural abundance | 1 mM | |
16 | Nrd1-Nab3 chimera | natural abundance | 1000 uM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.1
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
17 | sodium acetate | natural abundance | 10 mM | |
18 | sodium chloride | natural abundance | 25 mM | |
19 | DTT | natural abundance | 1 mM | |
20 | Nrd1-Nab3 chimera | natural abundance | 1000 uM |