Solution structure of the NRDI domain of Nab3
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 82.6 % (526 of 637) | 78.5 % (263 of 335) | 88.3 % (211 of 239) | 82.5 % (52 of 63) |
Backbone | 85.0 % (267 of 314) | 80.2 % (85 of 106) | 87.3 % (137 of 157) | 88.2 % (45 of 51) |
Sidechain | 81.8 % (306 of 374) | 77.7 % (178 of 229) | 91.0 % (121 of 133) | 58.3 % (7 of 12) |
Aromatic | 71.4 % (30 of 42) | 71.4 % (15 of 21) | 75.0 % (15 of 20) | 0.0 % (0 of 1) |
Methyl | 95.8 % (46 of 48) | 91.7 % (22 of 24) | 100.0 % (24 of 24) |
1. entity 1
GSWGSMENVN YDLLQKQVKY IMDSNMLNLP QFQHLPQEEK MSAILAMLNS NSDSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.6, Details 25 mM potassium phosphate, 25 mM sodium chloride, 1 mM DTT, 480 uM [U-100% 13C; U-100% 15N] Nab3, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | potassium phosphate | natural abundance | 25 mM | |
2 | sodium chloride | natural abundance | 25 mM | |
3 | DTT | natural abundance | 1 mM | |
4 | Nab3 | [U-100% 13C; U-100% 15N] | 480 uM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.6, Details 25 mM potassium phosphate, 25 mM sodium chloride, 1 mM DTT, 480 uM Nab3, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | potassium phosphate | natural abundance | 25 mM | |
6 | sodium chloride | natural abundance | 25 mM | |
7 | DTT | natural abundance | 1 mM | |
8 | Nab3 | natural abundance | 480 uM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.6, Details 25 mM potassium phosphate, 25 mM sodium chloride, 1 mM DTT, 480 uM Nab3, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | potassium phosphate | natural abundance | 25 mM | |
10 | sodium chloride | natural abundance | 25 mM | |
11 | DTT | natural abundance | 1 mM | |
12 | Nab3 | natural abundance | 480 uM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.6, Details 25 mM potassium phosphate, 25 mM sodium chloride, 1 mM DTT, 480 uM [U-100% 13C; U-100% 15N] Nab3, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | potassium phosphate | natural abundance | 25 mM | |
14 | sodium chloride | natural abundance | 25 mM | |
15 | DTT | natural abundance | 1 mM | |
16 | Nab3 | [U-100% 13C; U-100% 15N] | 480 uM |