Solution structure of an intramolecular RNA G-quadruplex formed by the 6A8U17U mutant from a 22mer guanine-rich sequence within the 5'UTR of BCL-2 proto onco-gene
Polymer type: polyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 96.5 % (334 of 346) | 94.1 % (176 of 187) | 99.3 % (141 of 142) | 100.0 % (17 of 17) |
Suger, PO4 | 99.6 % (241 of 242) | 100.0 % (132 of 132) | 99.1 % (109 of 110) | |
Nucleobase | 89.4 % (93 of 104) | 80.0 % (44 of 55) | 100.0 % (32 of 32) | 100.0 % (17 of 17) |
Aromatic | 94.9 % (93 of 98) | 89.8 % (44 of 49) | 100.0 % (32 of 32) | 100.0 % (17 of 17) |
1. entity 1
GGGCCAUUGG GUGGGAUCUG GGSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1.0 mM BCL-2 6A8U17U, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | BCL-2 6A8U17U | natural abundance | 1.0 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1.0 mM BCL-2 6A8U17U, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | BCL-2 6A8U17U | natural abundance | 1.0 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.5 mM G & U 15N-labeled BCL-2 6A8U17U, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | BCL-2 6A8U17U | [G & U 15N-labeled] | 0.5 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.5 mM G & U 15N-labeled BCL-2 6A8U17U, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | BCL-2 6A8U17U | [G & U 15N-labeled] | 0.5 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.5 mM A & C 15N-labeled BCL-2 6A8U17U, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | BCL-2 6A8U17U | [A & C 15N-labeled] | 0.5 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.5 mM A 13C, 15N-labeled BCL-2 6A8U17U, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | BCL-2 6A8U17U | [A 13C, 15N-labeled] | 0.5 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.3 mM C 13C, 15N-labeled BCL-2 6A8U17U, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | BCL-2 6A8U17U | [C 13C, 15N-labeled] | 0.3 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.3 mM G 13C-labeled BCL-2 6A8U17U, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | BCL-2 6A8U17U | [G 13C-labeled] | 0.3 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.5 mM U 13C, 15N-labeled BCL-2 6A8U17U, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | BCL-2 6A8U17U | [U 13C, 15N-labeled] | 0.5 mM |