Solution structure of an intramolecular RNA G-quadruplex formed by the 6A8A17U mutant from a 22mer guanine-rich sequence within the 5'UTR of BCL-2 proto-oncogene
Polymer type: polyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 92.4 % (318 of 344) | 94.6 % (176 of 186) | 100.0 % (142 of 142) | 0.0 % (0 of 16) |
Suger, PO4 | 100.0 % (242 of 242) | 100.0 % (132 of 132) | 100.0 % (110 of 110) | |
Nucleobase | 74.5 % (76 of 102) | 81.5 % (44 of 54) | 100.0 % (32 of 32) | 0.0 % (0 of 16) |
Aromatic | 79.2 % (76 of 96) | 91.7 % (44 of 48) | 100.0 % (32 of 32) | 0.0 % (0 of 16) |
1. entity 1
GGGCCAUAGG GUGGGAUCUG GGSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1.3 mM BCL-2 6A8A17U, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | BCL-2 6A8A17U | natural abundance | 1.3 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1.3 mM BCL-2 6A8A17U, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | BCL-2 6A8A17U | natural abundance | 1.3 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.5 mM G 15N-labeled BCL-2 6A8A17U, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | BCL-2 6A8A17U | [G 15N-labeled] | 0.5 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.3 mM A 13C, 15N-labeled BCL-2 6A8A17U, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | BCL-2 6A8A17U | [A 13C, 15N-labeled] | 0.3 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.3 mM C 13C, 15N-labeled BCL-2 6A8A17U, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | BCL-2 6A8A17U | [C 13C, 15N-labeled] | 0.3 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.5 mM G 13C-labeled BCL-2 6A8A17U, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | BCL-2 6A8A17U | [G 13C-labeled] | 0.5 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.3 mM U 13C, 15N-labeled BCL-2 6A8A17U, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | BCL-2 6A8A17U | [U 13C, 15N-labeled] | 0.3 mM |