Solution structure of the C terminal domain of MgtC (PA4635) from Pseudomonas aeruginosa
GPHMASEAEQ RYEVQIVCRA EDEIQVRSLM LHSLGSSDLR LQSLHSEDLD NPAKLEVRAE LLGTPEAPAQ LERLVSRVSL EKGVSSVRWQ VFELAAD
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 76.2 % (844 of 1108) | 91.6 % (525 of 573) | 52.1 % (222 of 426) | 89.0 % (97 of 109) |
Backbone | 86.1 % (494 of 574) | 94.8 % (184 of 194) | 77.0 % (221 of 287) | 95.7 % (89 of 93) |
Sidechain | 64.1 % (402 of 627) | 90.0 % (341 of 379) | 22.8 % (53 of 232) | 50.0 % (8 of 16) |
Aromatic | 40.5 % (17 of 42) | 76.2 % (16 of 21) | 0.0 % (0 of 20) | 100.0 % (1 of 1) |
Methyl | 56.7 % (68 of 120) | 100.0 % (60 of 60) | 13.3 % (8 of 60) |
1. entity 1
GPHMASEAEQ RYEVQIVCRA EDEIQVRSLM LHSLGSSDLR LQSLHSEDLD NPAKLEVRAE LLGTPEAPAQ LERLVSRVSL EKGVSSVRWQ VFELAADSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8, Details 0.4 mM [U-15N] PA4635, 25 mM sodium phosphate, 150 mM sodium chloride, 2 mM DTT, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | PA4635 | [U-15N] | 0.4 mM | |
2 | sodium phosphate | natural abundance | 25 mM | |
3 | sodium chloride | natural abundance | 150 mM | |
4 | DTT | natural abundance | 2 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8, Details 0.4 mM [U-13C; U-15N] PA4635, 25 mM sodium phosphate, 150 mM sodium chloride, 2 mM DTT, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | PA4635 | [U-13C; U-15N] | 0.4 mM | |
6 | sodium phosphate | natural abundance | 25 mM | |
7 | sodium chloride | natural abundance | 150 mM | |
8 | DTT | natural abundance | 2 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8, Details 0.4 mM [U-13C; U-15N] PA4635, 25 mM sodium phosphate, 150 mM sodium chloride, 2 mM DTT, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | PA4635 | [U-13C; U-15N] | 0.4 mM | |
10 | sodium phosphate | natural abundance | 25 mM | |
11 | sodium chloride | natural abundance | 150 mM | |
12 | DTT | natural abundance | 2 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8, Details 0.4 mM [U-13C; U-15N] PA4635, 25 mM sodium phosphate, 150 mM sodium chloride, 2 mM DTT, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | PA4635 | [U-13C; U-15N] | 0.4 mM | |
14 | sodium phosphate | natural abundance | 25 mM | |
15 | sodium chloride | natural abundance | 150 mM | |
16 | DTT | natural abundance | 2 mM |