BMRBj - BMREntryMaster

Found 1 Document (0.788 seconds)

BMRB: 34686

7QBY

Refined structure of the T193A mutant in the C-terminal domain of DNAJB6b

Authors

Karamanos, T.K., Cawood, E.E.

Assembly

DNAJ HOMOLOG SUBFAMILY B MEMBER 6 C-TERMINAL DOMAIN

Entity

1. DNAJ HOMOLOG SUBFAMILY B MEMBER 6 C-TERMINAL DOMAIN (polymer, Thiol state: not present), 57 monomers, 6433.288 Da Detail

MGNFKSISAS TKMVNGRKIT TKRIVENGQE RVEVEEDGQL KSLTINGKEQ LLRLDNK

Formula weight

6433.288 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.7 %, Completeness: 31.4 %, Completeness (bb): 57.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	31.4 % (214 of 681)	16.1 % (58 of 360)	41.7 % (105 of 252)	73.9 % (51 of 69)
Backbone	57.3 % (196 of 342)	37.0 % (44 of 119)	60.8 % (101 of 166)	89.5 % (51 of 57)
Sidechain	16.9 % (66 of 391)	5.8 % (14 of 241)	37.7 % (52 of 138)	0.0 % (0 of 12)
Aromatic	0.0 % (0 of 10)	0.0 % (0 of 5)	0.0 % (0 of 5)
Methyl	16.1 % (10 of 62)	16.1 % (5 of 31)	16.1 % (5 of 31)

1. entity 1

MGNFKSISAS TKMVNGRKIT TKRIVENGQE RVEVEEDGQL KSLTINGKEQ LLRLDNK

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 288 K, pH 6.7, Details 0.2 mM [U-13C; U-15N] DNAJ HOMOLOG SUBFAMILY B MEMBER 6 C-TERMINAL DOMAIN, 20 mM sodium phosphate, 150 mM sodium chloride, 0.02 % v/v sodium azide, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	DNAJ HOMOLOG SUBFAMILY B MEMBER 6 C-TERMINAL DOMAIN	[U-13C; U-15N]	0.2 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	150 mM
4	sodium azide	natural abundance	0.02 % v/v

Release date

2022-03-22

Citation

Microsecond Backbone Motions Modulate the Oligomerization of the DNAJB6 Chaperone
Cawood, E.E., Clore, G.M., Karamanos, T.K.
Angew. Chem Int Ed Engl. (2022), 61, e202116403-e202116403, PubMed 35247211 , DOI 10.1002/anie.202116403 , Abstract

Related entities 1. DNAJ HOMOLOG SUBFAMILY B MEMBER 6 C-TERMINAL DOMAIN, : 1 : 6 : 35 entities Detail

Interaction partners 1. DNAJ HOMOLOG SUBFAMILY B MEMBER 6 C-TERMINAL DOMAIN, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 4 experiments Detail

1 3D 1H-13C NOESY HCH

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 288 K, pH 6.7, Details 0.2 mM [U-13C; U-15N] DNAJ HOMOLOG SUBFAMILY B MEMBER 6 C-TERMINAL DOMAIN, 20 mM sodium phosphate, 150 mM sodium chloride, 0.02 % v/v sodium azide, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	DNAJ HOMOLOG SUBFAMILY B MEMBER 6 C-TERMINAL DOMAIN	[U-13C; U-15N]	0.2 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	150 mM
4	sodium azide	natural abundance	0.02 % v/v

2 3D 1H-13C NOESY CCH

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DNAJ HOMOLOG SUBFAMILY B MEMBER 6 C-TERMINAL DOMAIN	[U-13C; U-15N]	0.2 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	150 mM
4	sodium azide	natural abundance	0.02 % v/v

3 JHNHA ARTSY

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DNAJ HOMOLOG SUBFAMILY B MEMBER 6 C-TERMINAL DOMAIN	[U-13C; U-15N]	0.2 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	150 mM
4	sodium azide	natural abundance	0.02 % v/v

4 3D HNCACB

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DNAJ HOMOLOG SUBFAMILY B MEMBER 6 C-TERMINAL DOMAIN	[U-13C; U-15N]	0.2 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	150 mM
4	sodium azide	natural abundance	0.02 % v/v

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34686_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34686_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	OK	204		94.7 (chain: 1, length: 57)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 57, Sequence coverage: 94.7%
- Total number of assignments
  - ¹³C chemical shifts: 101
  - ¹⁵N chemical shifts: 50
  - ¹H chemical shifts: 53
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	252	101	40.1
¹⁵N chemical shifts	69	50	72.5
¹H chemical shifts	360	51	14.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	114	53	46.5
¹⁵N chemical shifts	57	50	87.7
¹H chemical shifts	119	40	33.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	138	48	34.8
¹⁵N chemical shifts	12	0	0.0
¹H chemical shifts	241	11	4.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	33	1	3.0
¹H chemical shifts	33	1	3.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	5	0	0.0
¹H chemical shifts	5	0	0.0

Keywords CHAPERONE, Hsp40 chaperone, anti-aggregation

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Found 1 Document (0.788 seconds)

BMRB: 34686

Sample

Related entities 1. DNAJ HOMOLOG SUBFAMILY B MEMBER 6 C-TERMINAL DOMAIN, : 1 : 6 : 35 entities Detail

Interaction partners 1. DNAJ HOMOLOG SUBFAMILY B MEMBER 6 C-TERMINAL DOMAIN, : 1 interactors Detail

Experiments performed 4 experiments Detail

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Instrument

Sample

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Chemical shift validation 3 contents Detail