Magic-angle spinning NMR structure of the human voltage-dependent anion channel 1 (E73V/C127A/C232S) in DMPC lipid bilayers
MAVPPTYADL GKSARDVFTK GYGFGLIKLD LKTKSENGLE FTSSGSANTE TTKVTGSLET KYRWTEYGLT FTVKWNTDNT LGTEITVEDQ LARGLKLTFD SSFSPNTGKK NAKIKTGYKR EHINLGADMD FDIAGPSIRG ALVLGYEGWL AGYQMNFETA KSRVTQSNFA VGYKTDEFQL HTNVNDGTEF GGSIYQKVNK KLETAVNLAW TAGNSNTRFG IAAKYQIDPD ASFSAKVNNS SLIGLGYTQT LKPGIKLTLS ALLDGKNVNA GGHKLGLGLE FQALEHHHHH H
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 36.7 % (1224 of 3333) | 25.1 % (429 of 1711) | 45.4 % (590 of 1299) | 63.5 % (205 of 323) |
Backbone | 65.5 % (1135 of 1734) | 59.7 % (363 of 608) | 67.5 % (568 of 841) | 71.6 % (204 of 285) |
Sidechain | 14.0 % (261 of 1858) | 6.1 % (67 of 1103) | 26.9 % (193 of 717) | 2.6 % (1 of 38) |
Aromatic | 1.9 % (6 of 312) | 2.6 % (4 of 156) | 1.3 % (2 of 152) | 0.0 % (0 of 4) |
Methyl | 12.0 % (38 of 316) | 7.0 % (11 of 158) | 17.1 % (27 of 158) |
1. entity 1
MAVPPTYADL GKSARDVFTK GYGFGLIKLD LKTKSENGLE FTSSGSANTE TTKVTGSLET KYRWTEYGLT FTVKWNTDNT LGTEITVEDQ LARGLKLTFD SSFSPNTGKK NAKIKTGYKR EHINLGADMD FDIAGPSIRG ALVLGYEGWL AGYQMNFETA KSRVTQSNFA VGYKTDEFQL HTNVNDGTEF GGSIYQKVNK KLETAVNLAW TAGNSNTRFG IAAKYQIDPD ASFSAKVNNS SLIGLGYTQT LKPGIKLTLS ALLDGKNVNA GGHKLGLGLE FQALEHHHHH HSolvent system 100% H2O, Pressure 1 atm, Temperature 285 K, pH 6.5, Details 66 % w/w [U-13C; U-15N; U-2H] u[2H,13C,15N]-hVDAC1(E73V/C127A/C232S), 33 % w/w DMPC, 10 mM MES, 150 mM sodium chloride, 20 mM MgCl2, 100% H2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | u[2H,13C,15N]-hVDAC1(E73V/C127A/C232S) | [U-13C; U-15N; U-2H] | 66 % w/w | |
2 | DMPC | natural abundance | 33 % w/w | |
3 | MES | natural abundance | 10 mM | |
4 | sodium chloride | natural abundance | 150 mM | |
5 | MgCl2 | natural abundance | 20 mM |
Solvent system 100% H2O, Pressure 1 atm, Temperature 285 K, pH 6.5, Details 66 % w/w [U-13C; U-15N]-alpha PET u[2H,13C,15N]-hVDAC1(E73V/C127A/C232S), 33 % w/w DMPC, 10 mM MES, 150 mM sodium chloride, 20 mM MgCl2, 100% H2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | u[2H,13C,15N]-hVDAC1(E73V/C127A/C232S) | [U-13C; U-15N]-alpha PET | 66 % w/w | |
7 | DMPC | natural abundance | 33 % w/w | |
8 | MES | natural abundance | 10 mM | |
9 | sodium chloride | natural abundance | 150 mM | |
10 | MgCl2 | natural abundance | 20 mM |