BMRB: 34700

GB1 in mammalian cells, 50 uM

Authors

Gerez, J.A., Prymaczok, N.C., Kadavath, H., Gosh, D., Butikofer, M., Guntert, P., Riek, R.

Assembly

Immunoglobulin G-binding protein G

Entity

1. Immunoglobulin G-binding protein G (polymer, Thiol state: not present), 57 monomers, 6353.953 Da Detail

MMQYKLILNG KTLKGETTTE AVDAATAEKV FKQYANDNGV DGEWTYDDAT KTFTVTE

Formula weight

6353.953 Da

Source organism

Streptococcus sp. 'group G'

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chemcal Shifts

¹H: 372 assignments Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val	Val err	Ambig. code
1	1	2	MET	HA	H	3.614	0.004
2	1	2	MET	HG3	H	1.896	0.000
3	1	2	MET	HE1	H	1.893	0.003
4	1	2	MET	HE2	H	1.893	0.003
5	1	2	MET	HE3	H	1.893	0.003
6	1	3	GLN	H	H	8.355	0.000
7	1	3	GLN	HA	H	5.311	0.000
8	1	3	GLN	HB2	H	1.788	0.003
9	1	3	GLN	HB3	H	1.788	0.003
10	1	3	GLN	HG2	H	2.188	0.009
11	1	3	GLN	HG3	H	1.938	0.000
12	1	3	GLN	HE21	H	7.848	0.000
13	1	3	GLN	HE22	H	6.868	0.006
14	1	4	TYR	H	H	9.325	0.000
15	1	4	TYR	HA	H	5.266	0.000
16	1	4	TYR	HB2	H	2.586	0.000
17	1	4	TYR	HB3	H	3.269	0.000
18	1	4	TYR	HD1	H	6.962	0.000
19	1	5	LYS	H	H	9.008	0.000
20	1	5	LYS	HA	H	5.097	0.000
21	1	5	LYS	HB2	H	1.768	0.000
22	1	5	LYS	HB3	H	1.868	0.000
23	1	5	LYS	HG2	H	1.177	0.000
24	1	5	LYS	HG3	H	1.265	0.000
25	1	5	LYS	HD2	H	1.446	0.000
26	1	5	LYS	HD3	H	1.502	0.024
27	1	5	LYS	HE2	H	2.620	0.000
28	1	5	LYS	HE3	H	2.740	0.024
29	1	6	LEU	H	H	8.479	0.000
30	1	6	LEU	HA	H	5.101	0.000
31	1	6	LEU	HB2	H	-1.341	0.000
32	1	6	LEU	HB3	H	0.620	0.002
33	1	6	LEU	HG	H	0.781	0.000
34	1	6	LEU	HD11	H	0.369	0.000
35	1	6	LEU	HD12	H	0.369	0.000
36	1	6	LEU	HD13	H	0.369	0.000
37	1	6	LEU	HD21	H	0.437	0.000
38	1	6	LEU	HD22	H	0.437	0.000
39	1	6	LEU	HD23	H	0.437	0.000
40	1	7	ILE	H	H	9.020	0.000
41	1	7	ILE	HA	H	4.262	0.000
42	1	7	ILE	HB	H	1.847	0.000
43	1	7	ILE	HG12	H	0.938	0.000
44	1	7	ILE	HG13	H	1.403	0.000
45	1	7	ILE	HG21	H	0.639	0.005
46	1	7	ILE	HG22	H	0.639	0.005
47	1	7	ILE	HG23	H	0.639	0.005
48	1	7	ILE	HD11	H	0.641	0.000
49	1	7	ILE	HD12	H	0.641	0.000
50	1	7	ILE	HD13	H	0.641	0.000
51	1	8	LEU	H	H	8.608	0.000
52	1	8	LEU	HA	H	4.273	0.000
53	1	8	LEU	HB2	H	1.262	0.000
54	1	8	LEU	HB3	H	1.358	0.023
55	1	8	LEU	HG	H	1.160	0.001
56	1	8	LEU	HD11	H	0.685	0.005
57	1	8	LEU	HD12	H	0.685	0.005
58	1	8	LEU	HD13	H	0.685	0.005
59	1	8	LEU	HD21	H	0.607	0.000
60	1	8	LEU	HD22	H	0.607	0.000
61	1	8	LEU	HD23	H	0.607	0.000
62	1	9	ASN	H	H	8.819	0.000
63	1	9	ASN	HA	H	5.146	0.000
64	1	9	ASN	HB2	H	2.366	0.000
65	1	9	ASN	HB3	H	2.867	0.000
66	1	9	ASN	HD21	H	6.708	0.000
67	1	9	ASN	HD22	H	7.100	0.001
68	1	10	GLY	H	H	7.848	0.000
69	1	10	GLY	HA2	H	3.983	0.000
70	1	10	GLY	HA3	H	4.278	0.008
71	1	11	LYS	H	H	9.414	0.000
72	1	11	LYS	HA	H	3.912	0.000
73	1	11	LYS	HB2	H	1.669	0.000
74	1	11	LYS	HB3	H	1.685	0.000
75	1	11	LYS	HG2	H	1.316	0.000
76	1	11	LYS	HG3	H	1.358	0.000
77	1	11	LYS	HD2	H	1.516	0.000
78	1	11	LYS	HD3	H	1.568	0.000
79	1	11	LYS	HE2	H	2.844	0.000
80	1	11	LYS	HE3	H	2.813	0.000
81	1	12	THR	H	H	8.755	0.000
82	1	12	THR	HA	H	4.245	0.000
83	1	12	THR	HB	H	4.085	0.000
84	1	12	THR	HG21	H	0.982	0.000
85	1	12	THR	HG22	H	0.982	0.000
86	1	12	THR	HG23	H	0.982	0.000
87	1	13	LEU	H	H	7.266	0.000
88	1	13	LEU	HA	H	4.322	0.000
89	1	13	LEU	HB2	H	1.265	0.000
90	1	13	LEU	HB3	H	1.416	0.000
91	1	13	LEU	HG	H	1.254	0.000
92	1	13	LEU	HD11	H	0.641	0.000
93	1	13	LEU	HD12	H	0.641	0.000
94	1	13	LEU	HD13	H	0.641	0.000
95	1	13	LEU	HD21	H	0.731	0.000
96	1	13	LEU	HD22	H	0.731	0.000
97	1	13	LEU	HD23	H	0.731	0.000
98	1	14	LYS	H	H	8.081	0.000
99	1	14	LYS	HA	H	5.095	0.000
100	1	14	LYS	HB2	H	1.575	0.000
101	1	14	LYS	HB3	H	1.744	0.000
102	1	14	LYS	HG2	H	1.353	0.000
103	1	14	LYS	HG3	H	1.303	0.000
104	1	14	LYS	HD2	H	1.565	0.000
105	1	14	LYS	HD3	H	1.608	0.000
106	1	14	LYS	HE2	H	2.765	0.000
107	1	14	LYS	HE3	H	2.854	0.000
108	1	15	GLY	H	H	8.356	0.006
109	1	15	GLY	HA2	H	3.996	0.000
110	1	15	GLY	HA3	H	4.185	0.000
111	1	16	GLU	H	H	8.380	0.000
112	1	16	GLU	HA	H	5.440	0.000
113	1	16	GLU	HB2	H	1.850	0.000
114	1	16	GLU	HB3	H	1.766	0.000
115	1	16	GLU	HG2	H	2.102	0.000
116	1	16	GLU	HG3	H	1.975	0.000
117	1	17	THR	H	H	8.644	0.000
118	1	17	THR	HA	H	4.573	0.000
119	1	17	THR	HB	H	3.753	0.000
120	1	17	THR	HG21	H	0.220	0.004
121	1	17	THR	HG22	H	0.220	0.004
122	1	17	THR	HG23	H	0.220	0.004
123	1	18	THR	H	H	7.962	0.000
124	1	18	THR	HA	H	5.708	0.000
125	1	18	THR	HB	H	4.177	0.000
126	1	18	THR	HG21	H	1.051	0.004
127	1	18	THR	HG22	H	1.051	0.004
128	1	18	THR	HG23	H	1.051	0.004
129	1	19	THR	H	H	8.895	0.000
130	1	19	THR	HA	H	4.546	0.000
131	1	19	THR	HB	H	3.684	0.000
132	1	19	THR	HG21	H	0.323	0.000
133	1	19	THR	HG22	H	0.323	0.000
134	1	19	THR	HG23	H	0.323	0.000
135	1	20	GLU	H	H	7.872	0.007
136	1	20	GLU	HA	H	4.976	0.000
137	1	20	GLU	HB2	H	1.831	0.000
138	1	20	GLU	HB3	H	1.810	0.000
139	1	20	GLU	HG2	H	2.180	0.000
140	1	20	GLU	HG3	H	2.135	0.023
141	1	21	ALA	H	H	9.031	0.000
142	1	21	ALA	HA	H	4.811	0.000
143	1	21	ALA	HB1	H	1.180	0.024
144	1	21	ALA	HB2	H	1.180	0.024
145	1	21	ALA	HB3	H	1.180	0.024
146	1	22	VAL	H	H	8.404	0.000
147	1	22	VAL	HA	H	3.965	0.001
148	1	22	VAL	HB	H	2.051	0.000
149	1	22	VAL	HG11	H	0.862	0.000
150	1	22	VAL	HG12	H	0.862	0.000
151	1	22	VAL	HG13	H	0.862	0.000
152	1	22	VAL	HG21	H	0.877	0.000
153	1	22	VAL	HG22	H	0.877	0.000
154	1	22	VAL	HG23	H	0.877	0.000
155	1	23	ASP	H	H	7.208	0.000
156	1	23	ASP	HA	H	4.603	0.000
157	1	23	ASP	HB2	H	2.849	0.000
158	1	23	ASP	HB3	H	2.943	0.000
159	1	24	ALA	H	H	8.210	0.000
160	1	24	ALA	HA	H	3.155	0.000
161	1	24	ALA	HB1	H	1.049	0.000
162	1	24	ALA	HB2	H	1.049	0.000
163	1	24	ALA	HB3	H	1.049	0.000
164	1	25	ALA	H	H	8.018	0.000
165	1	25	ALA	HA	H	3.810	0.000
166	1	25	ALA	HB1	H	1.141	0.000
167	1	25	ALA	HB2	H	1.141	0.000
168	1	25	ALA	HB3	H	1.141	0.000
169	1	26	THR	H	H	8.185	0.000
170	1	26	THR	HA	H	3.556	0.006
171	1	26	THR	HB	H	3.909	0.024
172	1	26	THR	HG21	H	1.072	0.000
173	1	26	THR	HG22	H	1.072	0.000
174	1	26	THR	HG23	H	1.072	0.000
175	1	27	ALA	H	H	7.030	0.000
176	1	27	ALA	HA	H	2.941	0.000
177	1	27	ALA	HB1	H	0.409	0.000
178	1	27	ALA	HB2	H	0.409	0.000
179	1	27	ALA	HB3	H	0.409	0.000
180	1	28	GLU	H	H	8.281	0.000
181	1	28	GLU	HA	H	2.849	0.000
182	1	28	GLU	HB2	H	1.683	0.000
183	1	28	GLU	HB3	H	1.752	0.000
184	1	28	GLU	HG2	H	1.517	0.000
185	1	28	GLU	HG3	H	1.440	0.000
186	1	29	LYS	H	H	6.794	0.001
187	1	29	LYS	HA	H	3.568	0.000
188	1	29	LYS	HB2	H	1.688	0.000
189	1	29	LYS	HB3	H	1.670	0.000
190	1	29	LYS	HG2	H	1.166	0.000
191	1	29	LYS	HG3	H	1.380	0.000
192	1	29	LYS	HD2	H	1.517	0.000
193	1	29	LYS	HD3	H	1.466	0.000
194	1	29	LYS	HE2	H	2.724	0.000
195	1	29	LYS	HE3	H	2.730	0.000
196	1	30	VAL	H	H	7.140	0.000
197	1	30	VAL	HA	H	3.461	0.000
198	1	30	VAL	HB	H	1.602	0.007
199	1	30	VAL	HG11	H	0.650	0.000
200	1	30	VAL	HG12	H	0.650	0.000
201	1	30	VAL	HG13	H	0.650	0.000
202	1	30	VAL	HG21	H	0.751	0.003
203	1	30	VAL	HG22	H	0.751	0.003
204	1	30	VAL	HG23	H	0.751	0.003
205	1	31	PHE	H	H	8.420	0.000
206	1	31	PHE	HA	H	4.345	0.000
207	1	31	PHE	HB2	H	2.775	0.021
208	1	31	PHE	HB3	H	3.254	0.024
209	1	31	PHE	HD1	H	6.585	0.000
210	1	31	PHE	HE1	H	7.054	0.000
211	1	31	PHE	HE2	H	7.014	0.000
212	1	31	PHE	HZ	H	7.178	0.000
213	1	32	LYS	H	H	8.989	0.000
214	1	32	LYS	HA	H	4.028	0.000
215	1	32	LYS	HB2	H	1.412	0.000
216	1	32	LYS	HB3	H	1.521	0.000
217	1	32	LYS	HG2	H	0.361	0.000
218	1	32	LYS	HG3	H	0.643	0.000
219	1	32	LYS	HD2	H	0.884	0.000
220	1	32	LYS	HD3	H	0.950	0.000
221	1	32	LYS	HE2	H	1.451	0.000
222	1	32	LYS	HE3	H	1.741	0.000
223	1	33	GLN	H	H	7.300	0.000
224	1	33	GLN	HA	H	3.889	0.000
225	1	33	GLN	HB2	H	2.048	0.000
226	1	33	GLN	HB3	H	2.078	0.000
227	1	33	GLN	HG2	H	2.266	0.000
228	1	33	GLN	HG3	H	2.289	0.000
229	1	33	GLN	HE21	H	6.832	0.000
230	1	33	GLN	HE22	H	7.898	0.000
231	1	34	TYR	H	H	8.173	0.000
232	1	34	TYR	HA	H	4.080	0.000
233	1	34	TYR	HB2	H	3.189	0.000
234	1	34	TYR	HB3	H	3.138	0.000
235	1	34	TYR	HD1	H	6.923	0.000
236	1	34	TYR	HD2	H	6.888	0.000
237	1	34	TYR	HE1	H	6.723	0.002
238	1	34	TYR	HE2	H	6.872	0.000
239	1	35	ALA	H	H	9.116	0.019
240	1	35	ALA	HA	H	3.671	0.000
241	1	35	ALA	HB1	H	1.703	0.000
242	1	35	ALA	HB2	H	1.703	0.000
243	1	35	ALA	HB3	H	1.703	0.000
244	1	36	ASN	H	H	8.106	0.001
245	1	36	ASN	HA	H	4.319	0.016
246	1	36	ASN	HB2	H	2.824	0.000
247	1	36	ASN	HB3	H	2.788	0.004
248	1	36	ASN	HD21	H	6.883	0.000
249	1	36	ASN	HD22	H	7.530	0.003
250	1	37	ASP	H	H	8.850	0.008
251	1	37	ASP	HA	H	4.219	0.000
252	1	37	ASP	HB2	H	2.564	0.000
253	1	37	ASP	HB3	H	2.421	0.000
254	1	38	ASN	H	H	7.285	0.000
255	1	38	ASN	HA	H	4.447	0.000
256	1	38	ASN	HB2	H	1.950	0.000
257	1	38	ASN	HB3	H	2.585	0.023
258	1	38	ASN	HD21	H	6.229	0.000
259	1	38	ASN	HD22	H	6.563	0.000
260	1	39	GLY	H	H	7.684	0.000
261	1	39	GLY	HA2	H	3.793	0.000
262	1	40	VAL	H	H	8.004	0.000
263	1	40	VAL	HA	H	4.011	0.000
264	1	40	VAL	HB	H	1.584	0.000
265	1	40	VAL	HG11	H	0.521	0.000
266	1	40	VAL	HG12	H	0.521	0.000
267	1	40	VAL	HG13	H	0.521	0.000
268	1	40	VAL	HG21	H	0.689	0.000
269	1	40	VAL	HG22	H	0.689	0.000
270	1	40	VAL	HG23	H	0.689	0.000
271	1	41	ASP	H	H	8.583	0.000
272	1	41	ASP	HA	H	4.825	0.000
273	1	41	ASP	HB2	H	2.464	0.021
274	1	41	ASP	HB3	H	2.654	0.024
275	1	42	GLY	H	H	7.660	0.000
276	1	42	GLY	HA2	H	3.444	0.000
277	1	42	GLY	HA3	H	4.204	0.000
278	1	43	GLU	H	H	8.032	0.000
279	1	43	GLU	HA	H	4.549	0.012
280	1	43	GLU	HB2	H	1.815	0.000
281	1	43	GLU	HB3	H	1.912	0.000
282	1	43	GLU	HG2	H	2.164	0.000
283	1	43	GLU	HG3	H	2.206	0.000
284	1	44	TRP	H	H	9.306	0.000
285	1	44	TRP	HA	H	5.190	0.000
286	1	44	TRP	HB2	H	3.014	0.000
287	1	44	TRP	HB3	H	3.296	0.000
288	1	44	TRP	HD1	H	7.444	0.000
289	1	44	TRP	HE1	H	10.436	0.000
290	1	44	TRP	HE3	H	7.556	0.000
291	1	44	TRP	HZ2	H	7.217	0.000
292	1	44	TRP	HZ3	H	6.480	0.000
293	1	44	TRP	HH2	H	6.623	0.000
294	1	45	THR	H	H	9.229	0.000
295	1	45	THR	HA	H	4.711	0.000
296	1	45	THR	HB	H	4.167	0.000
297	1	45	THR	HG21	H	1.045	0.000
298	1	45	THR	HG22	H	1.045	0.000
299	1	45	THR	HG23	H	1.045	0.000
300	1	46	TYR	H	H	8.518	0.000
301	1	46	TYR	HA	H	4.094	0.000
302	1	46	TYR	HB2	H	2.361	0.000
303	1	46	TYR	HB3	H	2.807	0.000
304	1	47	ASP	H	H	7.931	0.006
305	1	47	ASP	HA	H	4.460	0.000
306	1	47	ASP	HB2	H	2.087	0.000
307	1	47	ASP	HB3	H	2.488	0.021
308	1	48	ASP	H	H	8.513	0.000
309	1	48	ASP	HA	H	3.932	0.000
310	1	48	ASP	HB2	H	2.391	0.000
311	1	48	ASP	HB3	H	2.706	0.000
312	1	49	ALA	H	H	8.303	0.000
313	1	49	ALA	HA	H	3.955	0.009
314	1	49	ALA	HB1	H	1.355	0.000
315	1	49	ALA	HB2	H	1.355	0.000
316	1	49	ALA	HB3	H	1.355	0.000
317	1	50	THR	H	H	6.885	0.000
318	1	50	THR	HA	H	4.269	0.000
319	1	50	THR	HB	H	4.299	0.023
320	1	50	THR	HG21	H	0.927	0.000
321	1	50	THR	HG22	H	0.927	0.000
322	1	50	THR	HG23	H	0.927	0.000
323	1	51	LYS	H	H	7.730	0.000
324	1	51	LYS	HA	H	4.089	0.000
325	1	51	LYS	HB2	H	1.997	0.000
326	1	51	LYS	HB3	H	1.777	0.000
327	1	51	LYS	HG2	H	1.127	0.000
328	1	51	LYS	HG3	H	1.122	0.000
329	1	51	LYS	HD2	H	0.993	0.000
330	1	51	LYS	HD3	H	1.157	0.000
331	1	51	LYS	HE2	H	2.735	0.000
332	1	51	LYS	HE3	H	2.747	0.013
333	1	52	THR	H	H	7.240	0.000
334	1	52	THR	HA	H	5.381	0.000
335	1	52	THR	HB	H	3.611	0.000
336	1	52	THR	HG21	H	0.833	0.000
337	1	52	THR	HG22	H	0.833	0.000
338	1	52	THR	HG23	H	0.833	0.000
339	1	53	PHE	H	H	10.306	0.001
340	1	53	PHE	HA	H	5.547	0.000
341	1	53	PHE	HB2	H	3.123	0.000
342	1	53	PHE	HB3	H	3.131	0.000
343	1	53	PHE	HD1	H	7.764	0.000
344	1	53	PHE	HE1	H	7.152	0.000
345	1	53	PHE	HE2	H	7.111	0.000
346	1	53	PHE	HZ	H	6.928	0.023
347	1	54	THR	H	H	9.036	0.000
348	1	54	THR	HA	H	5.128	0.000
349	1	54	THR	HB	H	3.676	0.008
350	1	54	THR	HG21	H	0.809	0.000
351	1	54	THR	HG22	H	0.809	0.000
352	1	54	THR	HG23	H	0.809	0.000
353	1	55	VAL	H	H	8.040	0.000
354	1	55	VAL	HA	H	4.323	0.000
355	1	55	VAL	HG11	H	0.258	0.000
356	1	55	VAL	HG12	H	0.258	0.000
357	1	55	VAL	HG13	H	0.258	0.000
358	1	55	VAL	HG21	H	0.331	0.000
359	1	55	VAL	HG22	H	0.331	0.000
360	1	55	VAL	HG23	H	0.331	0.000
361	1	56	THR	H	H	8.258	0.000
362	1	56	THR	HA	H	4.539	0.000
363	1	56	THR	HB	H	3.715	0.000
364	1	56	THR	HG21	H	1.131	0.000
365	1	56	THR	HG22	H	1.131	0.000
366	1	56	THR	HG23	H	1.131	0.000
367	1	57	GLU	H	H	7.726	0.000
368	1	57	GLU	HA	H	4.148	0.000
369	1	57	GLU	HB2	H	1.780	0.000
370	1	57	GLU	HB3	H	2.044	0.000
371	1	57	GLU	HG2	H	2.233	0.000
372	1	57	GLU	HG3	H	2.206	0.000

Release date

2022-02-13

Citation

Protein structure determination in human cells by in-cell NMR and a reporter system to optimize protein delivery or transexpression
Gerez, J.A., Prymaczok, N.C., Kadavath, H., Ghosh, D., Butikofer, M., Fleischmann, Y., Guntert, P., Riek, R.
Commun. Biol. (2022), 5, 1322-1322, PubMed 36460747 , DOI 10.1038/s42003-022-04251-6 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Immunoglobulin G-binding protein G, : 1 : 2 : 67 : 38 entities Detail

Interaction partners 1. Immunoglobulin G-binding protein G, : 1 interactors Detail

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail