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BMRB: 34722

Structure of a parallel G-quadruplex with a snapback loop
Authors
Jana, J., Vianney, Y.M., Schroeder, N., Weisz, K.
Assembly
DNA (5'-D(*(DT5)P*GP*GP*CP*TP*AP*GP*GP*GP*TP*CP*AP*GP*GP*GP*TP*GP*GP*GP*TP*CP*AP*(DG3))-3')
Entity
1. DNA (5'-D(*(DT5)P*GP*GP*CP*TP*AP*GP*GP*GP*TP*CP*AP*GP*GP*GP*TP*GP*GP*GP*TP*CP*AP*(DG3))-3') (polymer, Thiol state: not present), 23 monomers, 7216.634 Da Detail

XGGCTAGGGT CAGGGTGGGT CAG


Formula weight
7216.634 Da
Source organism
synthetic construct
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 95.7 %, Completeness: 63.5 %, Completeness (bb): 57.1 % Detail
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Polymer type: polydeoxyribonucleotide

Total1H
All63.5 % (132 of 208)63.5 % (132 of 208)
Suger, PO457.1 % (88 of 154)57.1 % (88 of 154)
Nucleobase81.5 % (44 of 54)81.5 % (44 of 54)
Aromatic90.9 % (40 of 44)90.9 % (40 of 44)
Methyl100.0 % (4 of 4)100.0 % (4 of 4)

1. entity 1

XGGCTAGGGT CAGGGTGGGT CAG

Show sample condition
Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0, Details 0.3 mM DNA, 90% H2O/10% D2O


#NameIsotope labelingTypeConcentration
1DNAnatural abundance0.3 mM

Release date
2022-04-26
Citation
Guiding the folding of G-quadruplexes through loop residue interactions
Jana, J., Vianney, Y.M., Schroeder, N., Weisz, K.
Nucleic Acids Res. (2022), 50, 7161-7175, PubMed 35758626 , DOI 10.1093/nar/gkac549 ,
Experiments performed 3 experiments Detail
Chemical shift validation 3 contents Detail
Keywords DNA, G-quadruplex, parallel, snapback loop