Structure of the low complexity domain of TDP-43 (fragment 309-350) with methionine sulfoxide modifications
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 77.8 % (284 of 365) | 73.8 % (138 of 187) | 81.5 % (110 of 135) | 83.7 % (36 of 43) |
Backbone | 92.9 % (197 of 212) | 89.5 % (68 of 76) | 93.1 % (95 of 102) | 100.0 % (34 of 34) |
Sidechain | 62.8 % (115 of 183) | 63.1 % (70 of 111) | 68.3 % (43 of 63) | 22.2 % (2 of 9) |
Aromatic | 3.1 % (1 of 32) | 6.3 % (1 of 16) | 0.0 % (0 of 15) | 0.0 % (0 of 1) |
Methyl | 89.3 % (25 of 28) | 85.7 % (12 of 14) | 92.9 % (13 of 14) |
1. entity 1
GGXNFGAFSI NPAXXAAAQA ALQSSWGXXG XLASQQNQSG PSSolvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 288 (±0.2) K, pH 6.8 (±0.05), Details 500 uM [U-13C; U-15N] TDP-43 fragment 309-350, 20 mM HEPES, 10 mM potassium chloride, 5 mM MgCl2, 1 mM ATP, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TDP-43 fragment 309-350 | [U-13C; U-15N] | 500 uM | |
2 | HEPES | natural abundance | 20 mM | |
3 | potassium chloride | natural abundance | 10 mM | |
4 | MgCl2 | natural abundance | 5 mM | |
5 | ATP | natural abundance | 1 mM |